FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-56-6-5-8-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-56-6-5-8-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14733
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA769752.24326342224938No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGA5153.49555419805878No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA5043.4208918753817956No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAA4673.1697549718319418No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGA3092.097332518835268No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGA2611.77153329260843No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAA2581.7511708409692528No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAA2581.7511708409692528No Hit
TGATTGACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCA2431.6493585827733657No Hit
TGATTGACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGACGTGG2411.6357836150139144No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAA2091.4185841308626892No Hit
TGATTGACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAA2041.3846467114640602No Hit
TGATTGACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAA1901.2896219371478992No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAA1871.269259485508722No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGA1460.9909726464399647No Hit
TGATTGACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAA1350.916310323762981No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGA1240.8416480010859975No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAA1070.7262607751306591No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGA960.6515984524536754No Hit
TGATTGACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCC570.3868865811443698No Hit
TGATTGACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC560.380099097264644No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAA510.34616167786601504No Hit
TGATTGACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAA510.34616167786601504No Hit
TGATTGACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGACGTG440.29864929070793456No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAA430.2918618068282088No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAGA420.285074322948483No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTAA410.27828683906875723No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAA400.27149935518903145No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAA380.25792438742957985No Hit
TGATTGACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGACG370.2511369035498541No Hit
TGATTGACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGACG350.23756193579040247No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCT300.20362451639177356No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCCACGAGACACCATCCAATC280.190049548632322No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAA280.190049548632322No Hit
TGATTGACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAAC260.17647458087287043No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATA250.16968709699314463No Hit
TGATTGACACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCT250.16968709699314463No Hit
TGATTGACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAG240.16289961311341886No Hit
TGATTGACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGACG230.15611212923369305No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGA230.15611212923369305No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAA220.14932464535396728No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGA220.14932464535396728No Hit
TGATTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC200.13574967759451573No Hit
TGATTGACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAG200.13574967759451573No Hit
TGATTGACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGAC190.12896219371478992No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAA190.12896219371478992No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAG190.12896219371478992No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAG180.12217470983506415No Hit
TGATTGACACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC180.12217470983506415No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAA170.11538722595533836No Hit
TGATTGACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCTCAA170.11538722595533836No Hit
TGATTGATACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA170.11538722595533836No Hit
TGATTGACACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTAGACGT160.10859974207561257No Hit
TGATTGACACTGGGATTAGATACCCCACTATACTTAGCCCTAAACCTCAA150.10181225819588678No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAGCCTCAA150.10181225819588678No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATCAA150.10181225819588678No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGGG108.3803665E-4295.07
TGATTCA108.3803665E-4295.01
GACACTG14300.0295.06
GATTGAC14550.0295.02
TTGACAC14400.0295.04
CATGGGA108.3803665E-4295.08
TGATTGA14600.0293.989721
ATTGACA14650.0292.986363
ACACTGG14450.0292.958477
TGACACT14400.0292.95145
CACTGGG14400.0292.95148
ACTGGGA14400.0292.95149
ATGGGAT150.002821268196.666679
TGACATG150.002821268196.666675
TGACAGA150.002821268196.66667295
GATTCAC150.002821268196.666672
GACATGG200.006670652147.56
TTGACAT200.006670652147.54
CAGCCCT452.5465852E-1159.00000435-39
CAGCCCG150.00436423559.00000490-94
Produced by FastQC (version 0.11.5)