FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-55-5-4-1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-55-5-4-1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14756
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG406927.57522363784223No Hit
GGTACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTACC173911.785036595283275No Hit
GGTACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCACTCCTGT9266.275413391162917No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG7324.960693955001355No Hit
GGTACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACCACTCC5343.618866901599349No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG3012.0398481973434532No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC2972.012740580103009No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT2281.5451341827053402No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTACC2281.5451341827053402No Hit
GGTACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACCACTCCT1921.3011656275413392No Hit
GGTACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACCACTCCTG1861.2605042016806722No Hit
GGTACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACCACTCC1781.2062889671997832No Hit
GGTACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACCACTC1701.1520737327188941No Hit
GGTACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT1671.1317430197885605No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG1661.1249661154784494No Hit
GGTACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT1410.9555435077256709No Hit
GGTACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACCACTC1260.8538899430740038No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTACC1230.8335592301436703No Hit
GGTACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG1050.7115749525616698No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG1020.6912442396313364No Hit
GGTACACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTACCACT1000.6776904310111141No Hit
GGTACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTACCAC950.6438059094605584No Hit
GGTACACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTACCA920.623475196530225No Hit
GGTACACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTACCA920.623475196530225No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTACC840.5692599620493358No Hit
GGTACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTACCACTC800.5421523448088913No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG690.4676063973976688No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAC620.42016806722689076No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAC510.3456221198156682No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG500.3388452155055571No Hit
GGTACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG500.3388452155055571No Hit
GGTACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTACCACTC490.33206831119544594No Hit
GGTACACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTACC460.3117375982651125No Hit
GGTACACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTACCACTCC460.3117375982651125No Hit
GGTACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTACCA460.3117375982651125No Hit
GGTACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAC420.2846299810246679No Hit
GGTACACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTACCAC410.2778530767145568No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG410.2778530767145568No Hit
GGTACACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTACCACT410.2778530767145568No Hit
GGTACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG390.2642992680943345No Hit
GGTACACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTACC380.2575223637842234No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTACCA370.25074545947411225No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG370.25074545947411225No Hit
GGTACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCTA370.25074545947411225No Hit
GGTACACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTACCACT360.2439685551640011No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACTAGAGGAGCCTGTT360.2439685551640011No Hit
GGTACACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTACCACTCC360.2439685551640011No Hit
GGTACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT360.2439685551640011No Hit
GGTACACTGGGATTAGATACCCCACGGGACCTAGAGGAGCCTGTTCTACC350.23719165085388993No Hit
GGTACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTCT340.2304147465437788No Hit
GGTACACTGGGATTAGATACCCCGAGCCACGAGACCTAGAGGAGCCTGTT340.2304147465437788No Hit
GGTACACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTACCACTCC330.22363784223366767No Hit
GGTACACTGGGATTAGATACCCCGATCTAGAGGAGCCTGTTCTACCACTC330.22363784223366767No Hit
GGTACACTGGGATTAGATACCCCACTAACACTAGAGGAGCCTGTTCTACC310.21008403361344538No Hit
GGTACACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTACCACTCCT300.20330712930333425No Hit
GGTACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTACCA300.20330712930333425No Hit
GGTACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTACCA300.20330712930333425No Hit
GGTACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTACCACTCC300.20330712930333425No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG290.19653022499322312No Hit
GGTACACTGGGATTAGATACCCCTCAGAGCCCACGAGACCTAGAGGAGCC280.18975332068311196No Hit
GGTACACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTACC250.16942260775277854No Hit
GGTACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTACCACTCCT220.14909189482244511No Hit
GGTACACTGGGATTAGATACCCCCACGAACCTAGAGGAGCCTGTTCTACC220.14909189482244511No Hit
GGTACACTGGGATTAGATACCCCCGAGAAGACCTAGAGGAGCCTGTTCTA220.14909189482244511No Hit
GGTACACTGGGATTAGATACCCCCGCCACCTAGAGGAGCCTGTTCTACCA220.14909189482244511No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG220.14909189482244511No Hit
GGTACACTGGGATTAGATACCCCAGTAGTTCTAGAGGAGCCTGTTCTACC220.14909189482244511No Hit
GGTACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTACCACTCC210.14231499051233396No Hit
GGTACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTACCACTCC210.14231499051233396No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAACAG210.14231499051233396No Hit
GGTACACTGGGATTAGATACCCCCGACAACTAGAGGAGCCTGTTCTACCA200.13553808620222282No Hit
GGTACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT190.1287611818921117No Hit
GGTACACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTACCACTC190.1287611818921117No Hit
GGTACACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTACCA190.1287611818921117No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG180.12198427758200055No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAC170.1152073732718894No Hit
GGTACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGCC170.1152073732718894No Hit
GGTACACTGGGATTAGATACCCCAGCTAGAGGAGCCTGTTCTACCACTCC160.10843046896177824No Hit
GGTACACTGGGATTAGATACCCCAATGTCAGACTAGAGGAGCCTGTTCTA150.10165356465166712No Hit
GGTACACTGGGATTAGATACCCCGCCCTAGAGGAGCCTGTTCTACCACTC150.10165356465166712No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGAT14600.0295.07
ACACTGG14700.0295.04
CACTGGG14600.0295.05
ACTGGGA14600.0295.06
GGTACAC14850.0293.013461
TGGGATT14700.0292.99328
GGGATTA14700.0291.98989
TACACTG15000.0289.13
GTACACT15100.0287.185422
GGATAGA150.002821366196.666669
AAACCTT950.059.00000435-39
AAACCTC4400.059.00000435-39
AACCGCT452.5465852E-1159.00000460-64
CCGCTCG452.5465852E-1159.00000465-69
CCGCCAG1650.059.00000465-69
CGCTCGC452.5465852E-1159.00000465-69
ACCGCTC452.5465852E-1159.00000460-64
AACCTTG950.059.00000440-44
CATTCGC452.5465852E-1159.00000465-69
CCTTGAT950.059.00000440-44
Produced by FastQC (version 0.11.5)