FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-54-5-4-1-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-54-5-4-1-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14677
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG435129.64502282482796No Hit
GGTACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAGA13158.95959664781631No Hit
GGTACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGATTGAGACT9596.53403284049874No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG8085.50521223683314No Hit
GGTACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGATTGAG5393.6724126183825034No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAGA3762.5618314369421546No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC3442.343803229542822No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG3172.159841929549635No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT2221.5125706888328678No Hit
GGTACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGATTGAGAC2201.4989439258704096No Hit
GGTACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT2141.4580636369830346No Hit
GGTACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGATTGAGA1861.2672889555086189No Hit
GGTACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGATTGA1821.2400354295837024No Hit
GGTACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGATTGA1801.2264086666212441No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG1681.1446480888464945No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAGA1581.0765142740342033No Hit
GGTACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGATTGAG1511.0288206036655991No Hit
GGTACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT1010.6881515296041425No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAGA1000.6813381481229134No Hit
GGTACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG960.6540846221979969No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG920.6268310962730803No Hit
GGTACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAGAT890.606390951829393No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG810.5518838999795599No Hit
GGTACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGATT660.4496831777611229No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG600.4088028888737481No Hit
GGTACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGATTGA560.38154936294883146No Hit
GGTACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGATTGAG560.38154936294883146No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG500.3406690740614567No Hit
GGTACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAGAT490.33385569258022757No Hit
GGTACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG470.32022892961776933No Hit
GGTACACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTAGATTG470.32022892961776933No Hit
GGTACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG440.29978878517408186No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAG410.2793486407303945No Hit
GGTACACTGGGATTAGATACCCCCGTACGTGCAGGGAGGGTACCTTGAGG400.2725352592491654No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG390.2657218777679362No Hit
GGTACACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTAG380.2589084962867071No Hit
GGTACACTGGGATTAGATACCCCGTAGTCCTAGAGGAGCCTGTTCTAGAT370.2520951148054779No Hit
GGTACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT360.24528173332424882No Hit
GGTACACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTAGATT330.22484158888056144No Hit
GGTACACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTAGATTG330.22484158888056144No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAG310.21121482591810314No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG310.21121482591810314No Hit
GGTACACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAGATTGAG310.21121482591810314No Hit
GGTACACTGGGATTAGATACCCCCCGAGACCTAGAGGAGCCTGTTCTAGA290.19758806295564488No Hit
GGTACACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCTG290.19758806295564488No Hit
GGTACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT280.19077468147441573No Hit
GGTACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT280.19077468147441573No Hit
GGTACACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGATTG280.19077468147441573No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAG280.19077468147441573No Hit
GGTACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAG270.18396129999318664No Hit
GGTACACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAGATTG270.18396129999318664No Hit
GGTACACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAGAT270.18396129999318664No Hit
GGTACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGATTGAG260.1771479185119575No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG250.17033453703072834No Hit
GGTACACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAGA250.17033453703072834No Hit
GGTACACTGGGATTAGATACCCCGGGTAGTCTAGAGGAGCCTGTTCTAGA240.16352115554949923No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG240.16352115554949923No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACTAGAGGAGCCTGTT230.15670777406827008No Hit
GGTACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGAT230.15670777406827008No Hit
GGTACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGATTGAG230.15670777406827008No Hit
GGTACACTGGGATTAGATACCCCTGACCTAGAGGAGCCTGTTCTAGATTG220.14989439258704093No Hit
GGTACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGATTGAG220.14989439258704093No Hit
GGTACACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTTC210.1430810111058118No Hit
GGTACACTGGGATTAGATACCCCGACCCACGAGACCTAGAGGAGCCTGTT210.1430810111058118No Hit
GGTACACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAGAT200.1362676296245827No Hit
GGTACACTGGGATTAGATACCCCACACTAGAGGAGCCTGTTCTAGATTGA200.1362676296245827No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG200.1362676296245827No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTCG200.1362676296245827No Hit
GGTACACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAGATTG200.1362676296245827No Hit
GGTACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGCC200.1362676296245827No Hit
GGTACACTGGGATTAGATACCCCTGAGCCCACGAGACCTAGAGGAGCCTG190.12945424814335355No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAACAG190.12945424814335355No Hit
GGTACACTGGGATTAGATACCCCCGCCTAGAGGAGCCTGTTCTAGATTGA190.12945424814335355No Hit
GGTACACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTAGA180.12264086666212441No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTCAACAG180.12264086666212441No Hit
GGTACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGATTGAGA170.11582748518089528No Hit
GGTACACTGGGATTAGATACCCCTGACGCTAGAGGAGCCTGTTCTAGATT160.10901410369966613No Hit
GGTACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTCT160.10901410369966613No Hit
GGTACACTGGGATTAGATACCCCTAGTTGACTAGAGGAGCCTGTTCTAGA160.10901410369966613No Hit
GGTACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT150.10220072221843703No Hit
GGTACACTGGGATTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT150.10220072221843703No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAG157.0956503E-6295.09
CTGGGTT157.0956503E-6295.07
CTGGGAT14350.0295.07
CACTGGG14500.0295.05
GGGATTG108.380075E-4295.09
TGGGTTA157.0956503E-6295.08
ACTGGGT157.0956503E-6295.06
ACTGGGA14350.0295.06
GGTACAC14550.0295.01
TGGGATT14300.0293.968548
ACACTGG14600.0292.979464
GGGATTA14250.0292.92989
GTACACT14700.0291.98982
TACACTG14800.0290.016883
AAATGTA200.0066704215147.5295
AACGCTT452.5465852E-1159.00000490-94
TAAACCT5750.059.00000435-39
CCGCCAG1800.059.00000465-69
TCTAAGG452.5465852E-1159.000004100-104
ACCCCAA452.5465852E-1159.00000415-19
Produced by FastQC (version 0.11.5)