FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-53-4-4-1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-53-4-4-1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14797
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTACC238416.111373927147397No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG228015.408528755828884No Hit
GGTACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCACTCCTGT13218.927485301074542No Hit
GGTACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACCACTCC8115.480840710954923No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG3712.5072649861458403No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTACC3192.155842400486585No Hit
GGTACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACCACTCCT2972.007163614246131No Hit
GGTACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACCACTC2741.8517267013583836No Hit
GGTACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACCACTCC2621.7706291815908632No Hit
GGTACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACCACTCCTG2571.7368385483543962No Hit
GGTACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACCACTC2131.439480975873488No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTACC2071.3989322159897277No Hit
GGTACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT1931.3043184429276204No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC1841.2434953031019802No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG1511.020477123741299No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTACC1511.020477123741299No Hit
GGTACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTACCAC1390.9393796039737785No Hit
GGTACACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTACCA1380.932621477326485No Hit
GGTACACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTACCACT1380.932621477326485No Hit
GGTACACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTACCA1350.9123470973846051No Hit
GGTACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT1140.7704264377914443No Hit
GGTACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTACCACTC1020.6893289180239238No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAC990.6690545380820436No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG880.5947151449618165No Hit
GGTACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTACCACTC860.5811988916672298No Hit
GGTACACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTACCACT740.5001013718997094No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAC740.5001013718997094No Hit
GGTACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTACCA720.48658511860512266No Hit
GGTACACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTACC700.47306886531053594No Hit
GGTACACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTACC680.45955261201594916No Hit
GGTACACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTACCACTCC670.4527944853686558No Hit
GGTACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAC630.4257619787794823No Hit
GGTACACTGGGATTAGATACCCCGAGCCACGAGACCTAGAGGAGCCTGTT610.4122457254848956No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTACCA600.4054875988376022No Hit
GGTACACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTACCAC590.39872947219030885No Hit
GGTACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTACCA580.39197134554301544No Hit
GGTACACTGGGATTAGATACCCCACGGGACCTAGAGGAGCCTGTTCTACC570.38521321889572213No Hit
GGTACACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTACCACTCC570.38521321889572213No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAC570.38521321889572213No Hit
GGTACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCTA560.37845509224842877No Hit
GGTACACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTACCACT540.364938838953842No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG530.35818071230654863No Hit
GGTACACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTACCA490.33114820571737513No Hit
GGTACACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTACCACTCCT470.3176319524227884No Hit
GGTACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTACCA470.3176319524227884No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG460.31087382577549505No Hit
GGTACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG450.3041156991282017No Hit
GGTACACTGGGATTAGATACCCCAGTAGTTCTAGAGGAGCCTGTTCTACC450.3041156991282017No Hit
GGTACACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTACC430.2905994458336149No Hit
GGTACACTGGGATTAGATACCCCACTAACACTAGAGGAGCCTGTTCTACC420.28384131918632155No Hit
GGTACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTACCACTCC420.28384131918632155No Hit
GGTACACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTACCACTCC410.2770831925390282No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT410.2770831925390282No Hit
GGTACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTCT400.27032506589173483No Hit
GGTACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTACCACTCC380.25680881259714805No Hit
GGTACACTGGGATTAGATACCCCGATCTAGAGGAGCCTGTTCTACCACTC380.25680881259714805No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG370.2500506859498547No Hit
GGTACACTGGGATTAGATACCCCCGAGAAGACCTAGAGGAGCCTGTTCTA350.23653443265526797No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG340.22977630600797458No Hit
GGTACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTACCACTCCT330.22301817936068122No Hit
GGTACACTGGGATTAGATACCCCCACGAACCTAGAGGAGCCTGTTCTACC320.21626005271338783No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG320.21626005271338783No Hit
GGTACACTGGGATTAGATACCCCCGACAACTAGAGGAGCCTGTTCTACCA310.20950192606609447No Hit
GGTACACTGGGATTAGATACCCCGTCAGACTAGAGGAGCCTGTTCTACCA290.19598567277150772No Hit
GGTACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTACCACTCC280.18922754612421439No Hit
GGTACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG270.182469419476921No Hit
GGTACACTGGGATTAGATACCCCCGCCACCTAGAGGAGCCTGTTCTACCA260.17571129282962764No Hit
GGTACACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTACCACTC250.16895316618233427No Hit
GGTACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG240.16219503953504089No Hit
GGTACACTGGGATTAGATACCCCAGCTAGAGGAGCCTGTTCTACCACTCC230.15543691288774752No Hit
GGTACACTGGGATTAGATACCCCTCAGAGCCCACGAGACCTAGAGGAGCC230.15543691288774752No Hit
GGTACACTGGGATTAGATACCCCGCCCTAGAGGAGCCTGTTCTACCACTC220.14867878624045414No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG200.13516253294586741No Hit
GGTACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT190.12840440629857403No Hit
GGTACACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCCTA190.12840440629857403No Hit
GGTACACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTCTA190.12840440629857403No Hit
GGTACACTGGGATTAGATACCCCTATAAAGGGCTAGAGGAGCCTGTTCTA180.12164627965128066No Hit
GGTACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTACCA170.11488815300398729No Hit
GGTACACTGGGATTAGATACCCCATCAGACTAGAGGAGCCTGTTCTACCA170.11488815300398729No Hit
GGTACACTGGGATTAGATACCCCAGGAGACCTAGAGGAGCCTGTTCTACC170.11488815300398729No Hit
GGTACACTGGGATTAGATACCCCTCCTAGCCCACGAGACCTAGAGGAGCC160.10813002635669391No Hit
GGTACACTGGGATTAGATACCCCAAGGAGATCGGGGGCCTAGAGGAGCCT160.10813002635669391No Hit
GGTACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT150.10137189970940055No Hit
GGTACACTGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTACCA150.10137189970940055No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAG108.380657E-4295.09
CTGGGTT108.380657E-4295.07
CTGGGAT14500.0295.07
CACTGGG14600.0295.05
GGGATTA14550.0295.09
TGGGTTA108.380657E-4295.08
TGGGATT14500.0295.08
ACTGGGT108.380657E-4295.06
GGTACAC14750.0294.01
ACACTGG14700.0293.99664
ACTGGGA14550.0293.986276
GTACACT14850.0291.026952
TACACTG14850.0291.026953
GTACAAA550.001255598280.45455295
ACTGCTC2150.059.00000460-64
CCTGTAG550.059.00000420-24
GCGGTGC3750.059.000004110-114
AACCTTG550.059.00000440-44
CCCCGTC900.059.00000420-24
ACCCCGA550.059.00000415-19
Produced by FastQC (version 0.11.5)