FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-52-4-8-2-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-52-4-8-2-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14883
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA292419.646576631055567No Hit
CAACACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAA12858.63401195995431No Hit
CAACACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAACTTCGCCT10847.28347779345562No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA5813.9037828394812872No Hit
CAACACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAACTTCG4923.3057851239669422No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAA4372.9362359739299873No Hit
CAACACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAACTTC4312.8959215211986833No Hit
CAACACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC3142.109789692938252No Hit
CAACACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAACTTCGCC3012.0224417120204263No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA2581.7335214674460795No Hit
CAACACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAACTTCGC2561.7200833165356446No Hit
CAACACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAACTTCG2221.4916347510582544No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA2151.4446012228717329No Hit
CAACACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT1921.2900624874017335No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAA1761.1825572801182558No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA1651.1086474501108647No Hit
CAACACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA1581.0616139219243432No Hit
CAACACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAACTTC1521.021299469193039No Hit
CAACACACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTAA1330.8936370355439092No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAA1180.7928509037156486No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA1180.7928509037156486No Hit
CAACACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAAC1170.7861318282604314No Hit
CAACACACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAAC1130.759255526439562No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA980.6584693946113015No Hit
CAACACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAAC950.6383121682456494No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA890.5979977155143452No Hit
CAACACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC840.5644023382382585No Hit
CAACACACTGGGATTAGATACCCCACTGACACTAGAGGAGCCTGTTCTAA830.557683262783041No Hit
CAACACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA830.557683262783041No Hit
CAACACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAAC820.5509641873278237No Hit
CAACACACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAA770.5173688100517368No Hit
CAACACACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTA760.5106497345965195No Hit
CAACACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAACTTC690.46361620640999796No Hit
CAACACACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAACTT670.45017805549956325No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC640.43002082913391115No Hit
CAACACACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTAA620.4165826782234765No Hit
CAACACACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTAACT570.38298730094738964No Hit
CAACACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA510.34267284821608546No Hit
CAACACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAACTTCG510.34267284821608546No Hit
CAACACACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTAA500.3359537727608681No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA500.3359537727608681No Hit
CAACACACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTA490.32923469730565075No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA470.31579654639521604No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAAC470.31579654639521604No Hit
CAACACACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCT460.30907747093999866No Hit
CAACACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT460.30907747093999866No Hit
CAACACACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAGG460.30907747093999866No Hit
CAACACACTGGGATTAGATACCCCAATTAATACGACTCACTAGAGGAGCC430.28892024457434656No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA430.28892024457434656No Hit
CAACACACTGGGATTAGATACCCCTGAGCCCACGAGACCTAGAGGAGCCT420.28220116911912924No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG420.28220116911912924No Hit
CAACACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAACT410.27548209366391185No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA410.27548209366391185No Hit
CAACACACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCCT400.26876301820869447No Hit
CAACACACTGGGATTAGATACCCCGTAGTCCTAGAGGAGCCTGTTCTAAC390.2620439427534771No Hit
CAACACACTGGGATTAGATACCCCAATGAGATCGAGTGCCTAGAGGAGCC360.24188671638782502No Hit
CAACACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA350.23516764093260764No Hit
CAACACACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTCT340.22844856547739031No Hit
CAACACACTGGGATTAGATACCCCAGCCCACGAGACCTAGAGGAGCCTGT340.22844856547739031No Hit
CAACACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAACTTCG300.20157226365652087No Hit
CAACACACTGGGATTAGATACCCCACGCCTAGAGGAGCCTGTTCTAACTT260.17469596183565142No Hit
CAACACACTGGGATTAGATACCCCCATCTAGAGGAGCCTGTTCTAACTTC250.16797688638043404No Hit
CAACACACTGGGATTAGATACCCCAGGACCTAGAGGAGCCTGTTCTAACT250.16797688638043404No Hit
CAACACACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTAACTTCGC250.16797688638043404No Hit
CAACACACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTAACTT250.16797688638043404No Hit
CAACACACTGGGATTAGATACCCCGTCTTCTAGAGGAGCCTGTTCTAACT250.16797688638043404No Hit
CAACACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAACTTCGC240.16125781092521668No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA240.16125781092521668No Hit
CAACACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG240.16125781092521668No Hit
CAACACACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCTA240.16125781092521668No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA230.15453873546999933No Hit
CAACACACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAAC230.15453873546999933No Hit
CAACACACTGGGATTAGATACCCCCGGTAGTCCTAGAGGAGCCTGTTCTA230.15453873546999933No Hit
CAACACACTGGGATTAGATACCCCACGACCTAGAGGAGCCTGTTCTAACT230.15453873546999933No Hit
CAACACACTGGGATTAGATACCCCTGTATAAGAGGAGCCTGTTCTAACTT230.15453873546999933No Hit
CAACACACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATAT230.15453873546999933No Hit
CAACACACTGGGATTAGATACCCCGAGATGCCTAAGCTAGAGGAGCCTGT230.15453873546999933No Hit
CAACACACTGGGATTAGATACCCCAGAACTAGAGGAGCCTGTTCTAACTT220.14781966001478197No Hit
CAACACGCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA220.14781966001478197No Hit
CAACACACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAACTTCG220.14781966001478197No Hit
CAACACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAAC220.14781966001478197No Hit
CAACACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAACTTCG210.14110058455956462No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA200.13438150910434724No Hit
CAACACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG200.13438150910434724No Hit
CAACACACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAACTTCG200.13438150910434724No Hit
CAACACACTGGGATTAGATACCCCTGACGCTGCTAGAGGAGCCTGTTCTA190.12766243364912988No Hit
CAACACACTGGGATTAGATACCCCAAGCCCACGAGACCTAGAGGAGCCTG190.12766243364912988No Hit
CAACACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAACTTCG190.12766243364912988No Hit
CAACACACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTAACTT180.12094335819391251No Hit
CAACACACTGGGATTAGATACCCCTAAGAGGAGCCTGTTCTAACTTCGCC170.11422428273869516No Hit
CAACACACTGGGATTAGATACCCCGATCTAGAGGAGCCTGTTCTAACTTC160.10750520728347779No Hit
CAACACACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAACTT150.10078613182826043No Hit
CAACACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT150.10078613182826043No Hit
CAACACACTGGGATTAGATACCCCCCACCTAGAGGAGCCTGTTCTAACTT150.10078613182826043No Hit
CAACACACTGGGATTAGATACCCCTGGTGACGACTAGAGGAGCCTGTTCT150.10078613182826043No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGG157.097611E-6295.06
ACACGGG108.381232E-4295.05
CACACGG157.097611E-6295.04
CACGCTG157.097611E-6295.04
GCTGGGA157.097611E-6295.07
CACTGGG14300.0295.06
CTGGATT157.097611E-6295.08
TGGATTA157.097611E-6295.09
CTGGGAT14450.0293.979228
ACTGGGA14300.0293.968547
ACACTGG14550.0292.97255
TGGGATT14450.0292.958479
CACACTG14750.0290.04
ACACACT15600.0274.198733
AACACAC18500.0233.608112
CAACACA18900.0228.664021
ACACGCT202.2372049E-5221.253
ACGCTGG202.2372049E-5221.255
CACTGGA301.1265439E-4147.56
ACTGGAT301.1265439E-4147.57
Produced by FastQC (version 0.11.5)