FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-51-6-7-5-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-51-6-7-5-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14182
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1087676.68876040050769No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG3432.4185587364264562No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG2301.6217740798194893No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG1641.15639543082781No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG1591.1211394725708645No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG1200.8461429981666901No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG1200.8461429981666901No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC1130.7967846566069666No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG1100.7756310816527994No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG950.6698632068819631No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG860.6064024820194613No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG810.5711465237625158No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT710.5006346072486251No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAGACCTCAACAG700.4935834155972359No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT640.45127626568890145No Hit
AAGCGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT530.3737131575236215No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTCG510.3596107742208433No Hit
AAGCGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT380.26794528275278523No Hit
AAGCGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT300.21153574954167254No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATATC280.19743336623889435No Hit
AAGCGACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT260.1833309829361162No Hit
AAGCGATGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT240.16922859963333803No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACAG230.16217740798194893No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG210.14807502467917077No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAACCCTAAACCTCAACAG200.14102383302778168No Hit
AAGCGACTGGGATTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT190.13397264137639261No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGATGC180.12692144972500352No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAGATAA180.12692144972500352No Hit
AAGCGACTGGGATTAATACCCCACTATGCTTAGCCCTAAACCTCAACAGT170.11987025807361443No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACAG160.11281906642222536No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACGG150.10576787477083627No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGAT14000.0295.07
GACTGGG14050.0295.05
AAGCGAC14150.0295.01
TGGGATT13950.0295.08
ACTGGGA14050.0295.06
AGCGACT14100.0295.02
CGACTGG14100.0295.04
GCGACTG14150.0293.95763
GGGATTA14000.0293.946449
AGATAGA150.0028201512196.66667295
AACTATA550.001254528880.454544295
AAACCTT303.2976277E-759.00000435-39
AATCTTG303.2976277E-759.00000440-44
CCAATAG150.0043635659.00000440-44
AACCGCT303.2976277E-759.00000460-64
ATCTTGA303.2976277E-759.00000440-44
TAGGAGC150.0043635659.00000480-84
AAATCTT303.2976277E-759.00000435-39
CTTTACC303.2976277E-759.00000450-54
TTACTTT303.2976277E-759.00000450-54
Produced by FastQC (version 0.11.5)