FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-50-6-5-8-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-50-6-5-8-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14107
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA666347.23187070248812No Hit
TGATTGACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCTAGCT5643.998015169773871No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA5413.834975544056142No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGA5073.593960445169065No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAA4693.3245906287658613No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAA3312.3463528744594884No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGA2952.0911604168143474No Hit
TGATTGACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCA2621.857233997306302No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGA2361.6729283334514782No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAA1931.3681151201531154No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAA1851.311405685120862No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAA1841.3043170057418303No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAA1771.2546962500886085No Hit
TGATTGACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAA1441.0207698305805628No Hit
TGATTGACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAA1400.9924151130644361No Hit
TGATTGACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACCTAGC1300.9215283192741193No Hit
TGATTGACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAA1240.8789962429999292No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGA1130.8010207698305806No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCT800.5670943503225349No Hit
TGATTGACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCC580.4111434039838378No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAG540.3827886864677111No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGA510.361522648330616No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGA500.35443396895158436No Hit
TGATTGACACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTACCTAG430.30481321329836253No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAA430.30481321329836253No Hit
TGATTGACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAA420.29772453391933085No Hit
TGATTGACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACCTA400.28354717516126743No Hit
TGATTGACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC400.28354717516126743No Hit
TGATTGACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTACCTAG380.2693698164032041No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAA370.2622811370241724No Hit
TGATTGACACTGGGATTAGATACCCCGAGAGAGGAGCCTGTTCTACCTAG360.2551924576451407No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAGCCGTAAACTCAGA350.24810377826610902No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTA310.2197490607499823No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAA290.2055717019919189No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAGA260.18430566385482383No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGA250.17721698447579218No Hit
TGATTGACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAG230.1630396257177288No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAA220.1559509463386971No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAA210.14886226695966542No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCCA200.14177358758063371No Hit
TGATTGACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAAC200.14177358758063371No Hit
TGATTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC200.14177358758063371No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAA190.13468490820160206No Hit
TGATTGACACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTA190.13468490820160206No Hit
TGATTGACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAC190.13468490820160206No Hit
TGATTGACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCT190.13468490820160206No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCCACGAGACACCATCCAATC180.12759622882257035No Hit
TGATTGACACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC180.12759622882257035No Hit
TGATTGACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACCTA180.12759622882257035No Hit
TGATTGATACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA170.12050754944353867No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATCAA170.12050754944353867No Hit
TGATTGACACTGGGATTAGATACCCCTCCGAGCCCACGAGACACCATCCA160.11341887006450699No Hit
TGATTGACACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTACC160.11341887006450699No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGA150.10633019068547529No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAA150.10633019068547529No Hit
TGATTGACACTGGGATTAGATACCCCCGTCGGCACTAGAGGAGCCTGTTC150.10633019068547529No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGA150.10633019068547529No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATTGA13900.0295.01
TGACACT13750.0295.05
GACACTG13750.0295.06
GATTGAC13850.0295.02
CAGCTAA108.3767273E-4294.99997295
TGATTAC108.3767273E-4294.999971
GATTACA108.3767273E-4294.999972
ACTGGAA108.3767273E-4294.999979
TTGACAC13850.0293.935034
ATTGACA13850.0293.935033
CACTGGG13700.0292.84678
ACTGGGA13700.0292.84679
ACACTGG13900.0291.816537
TATATGA150.0028200452196.66667295
CACTGGA304.509966E-7196.666678
CTGACAA150.0028200452196.66667295
TACACTG150.0028200452196.666675
ACTGGAT150.0028200452196.666679
TTACACT200.00666777147.499984
ATCCCCC303.2974458E-759.000004125-129
Produced by FastQC (version 0.11.5)