FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-5-4-5-7-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-5-4-5-7-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11350
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC198017.444933920704848No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA152713.453744493392069No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATAAGCACT10078.872246696035242No Hit
CTGGATGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATAAGC6645.850220264317181No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT3503.0837004405286343No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA2892.5462555066079298No Hit
CTGGATGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATAAG2642.325991189427313No Hit
CTGGATGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTATAAG2081.8325991189427313No Hit
CTGGATGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATAAGCAC2041.7973568281938326No Hit
CTGGATGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC1561.3744493392070485No Hit
CTGGATGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTATAAGC1531.3480176211453745No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA1481.303964757709251No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT1451.277533039647577No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA1421.2511013215859033No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT1391.224669603524229No Hit
CTGGATGACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTATAA1251.1013215859030838No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTATAAGCA1241.0925110132158589No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT1231.0837004405286343No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT970.854625550660793No Hit
CTGGATGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAT950.8370044052863436No Hit
CTGGATGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTATAAG930.8193832599118943No Hit
CTGGATGACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTA870.7665198237885463No Hit
CTGGATGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT820.722466960352423No Hit
CTGGATGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTATAAG780.6872246696035242No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC670.5903083700440528No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTATA580.5110132158590308No Hit
CTGGATGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAT580.5110132158590308No Hit
CTGGATGACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTATAAGC560.4933920704845815No Hit
CTGGATGACTGGGATTAGATACCCCACGAACTAGAGGAGCCTGTTCTATA510.44933920704845814No Hit
CTGGATGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTATA460.4052863436123348No Hit
CTGGATGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTATAAGC450.39647577092511016No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC450.39647577092511016No Hit
CTGGATGACTGGGATTAGATACCCCAGTGCAGCTGTTGGAGACTAGAGGA450.39647577092511016No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT430.3788546255506608No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT430.3788546255506608No Hit
CTGGATGACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTA420.3700440528634361No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT370.32599118942731276No Hit
CTGGATGACTGGGATTAGATACCCCTGACTGCCAACTAGAGGAGCCTGTT370.32599118942731276No Hit
CTGGATGACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC370.32599118942731276No Hit
CTGGATGACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTATAAGC360.31718061674008813No Hit
CTGGATGACTGGGATTAGATACCCCGTGCTAGAGGAGCCTGTTCTATAAG360.31718061674008813No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTTCTAGAGGAGCCTGTTCTA360.31718061674008813No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT360.31718061674008813No Hit
CTGGATGACTGGGATTAGATACCCCACTAGACCTAGAGGAGCCTGTTCTA350.30837004405286345No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCACGAGACCTAGAGGAGCCTG350.30837004405286345No Hit
CTGGATGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT340.29955947136563876No Hit
CTGGATGACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTATAAGC340.29955947136563876No Hit
CTGGATGACTGGGATTAGATACCCCGCCCGACCTAGAGGAGCCTGTTCTA330.29074889867841414No Hit
CTGGATGACTGGGATTAGATACCCCACTATACACATTACGCTAGAGGAGC320.28193832599118945No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCT310.2731277533039647No Hit
CTGGATGACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACT310.2731277533039647No Hit
CTGGATGACTGGGATTAGATACCCCTACCCTAGAGGAGCCTGTTCTATAA300.2643171806167401No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGTCTAGAGGAGCCTGTTCTA280.24669603524229075No Hit
CTGGATGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTATAAGC280.24669603524229075No Hit
CTGGATGACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCT280.24669603524229075No Hit
CTGGATGACTGGGATTAGATACCCCTGCTGACTAGAGGAGCCTGTTCTAT270.2378854625550661No Hit
CTGGATGACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCC260.2290748898678414No Hit
CTGGATGACTGGGATTAGATACCCCATGTCAGACTAGAGGAGCCTGTTCT250.22026431718061676No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT250.22026431718061676No Hit
CTGGATGACTGGGATTAGATACCCCTATAAAGGGCTAGAGGAGCCTGTTC240.21145374449339205No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT240.21145374449339205No Hit
CTGGATGACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATA230.2026431718061674No Hit
CTGGATGACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAT220.19383259911894274No Hit
CTGGATGACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTA220.19383259911894274No Hit
CTGGATGACTGGGATTAGATACCCCTGACACCTAGAGGAGCCTGTTCTAT210.18502202643171806No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGGCCTAGAGGAGCCT210.18502202643171806No Hit
CTGGATGACTGGGATTAGATACCCCTGACGCTGCTAGAGGAGCCTGTTCT210.18502202643171806No Hit
CTGGATGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAT200.1762114537444934No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT190.16740088105726872No Hit
CTGGATGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTATAAGC180.15859030837004406No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC180.15859030837004406No Hit
CTGGATGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT170.14977973568281938No Hit
CTGGATGACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTATAAGCA170.14977973568281938No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAT150.13215859030837004No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT130.1145374449339207No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT130.1145374449339207No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCACCTAGAGGAGCCTGTTCTA120.10572687224669602No Hit
CTGGATGACTGGGATTAGATACCCCAGTCTAGAGGAGCCTGTTCTATAAG120.10572687224669602No Hit
CTGGATGACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTATAA120.10572687224669602No Hit
CTGGATGACTGGGATTAGATACCCCTGACTAGAGGAGCCTGTTCTATAAG120.10572687224669602No Hit
CTGGATGACTGGGATTAGATACCCCACGAGAACTAGAGGAGCCTGTTCTA120.10572687224669602No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGAC11300.0293.694673
TGGATGA11300.0293.694672
TGACTGG11150.0293.677126
GACTGGG11150.0293.677127
CTGGGAT11100.0293.671179
CTGGATG11350.0292.400881
GATGACT11300.0292.389374
ATGACTG11250.0292.377785
ACTGGGA11150.0292.354258
CCTGTAG452.5465852E-1159.00000420-24
GCCAGGG452.5465852E-1159.00000470-74
CTTGGCG950.059.000004110-114
CTGGCGG1800.059.000004110-114
CCCGTCT900.059.00000420-24
CCCCGTC900.059.00000420-24
ACCCCGG452.5465852E-1159.00000420-24
CCTCAGA452.5465852E-1159.000004120-124
AAGCGCC452.5465852E-1159.00000485-89
TTGGCGG900.059.000004110-114
GGGAACT452.5465852E-1159.00000475-79
Produced by FastQC (version 0.11.5)