FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-49-5-2-6-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-49-5-2-6-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14386
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA515335.81954678159322No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA11818.20937022104824No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAA6504.518281662727651No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAACTTCGCCT5453.7884053941331852No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA4903.406089253440845No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA4753.3018212150702073No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAACTTCG3732.592798554149868No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT2761.9185319060197417No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAA2581.7934102599749757No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA2531.758654247184763No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA1771.23036285277353No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA1380.9592659530098708No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAACTTC1340.9314611427777005No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAACTTCGCC1270.8828027248714028No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA1190.8271931044070624No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG1140.7924370916168497No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC1130.7854858890588071No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA1100.7646322813846795No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA1030.7159738634783818No Hit
GCCACAACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA1010.7020714583622967No Hit
GCCACAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAACTTCG890.6186570276657862No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA840.5839010148755734No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA810.5630474072014459No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAACTTCGC770.5352425969692757No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA760.5282913944112331No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAA740.5143889892951481No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAACTTC720.500486584179063No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA670.46573057138885027No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAA620.4309745585986376No Hit
GCCACAACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAA410.28499930487974423No Hit
GCCACAACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAAC400.27804810232170163No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT400.27804810232170163No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA390.27109689976365914No Hit
GCCACAACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAACT370.25719449464757405No Hit
GCCACAACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAAC370.25719449464757405No Hit
GCCACAACTGGGATTAGATACCCCCGTGGACTAGAGGAGCCTGTTCTAAC360.2502432920895315No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG360.2502432920895315No Hit
GCCACAACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAACTT350.24329208953148893No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA350.24329208953148893No Hit
GCCACAACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAACTTC340.2363408869734464No Hit
GCCACAACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATA330.22938968441540386No Hit
GCCACAACTGGGATTAGATACCCCTATAAAGGGCTAGAGGAGCCTGTTCT320.22243848185736131No Hit
GCCACAACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAACTTCG310.2154872792993188No Hit
GCCACAACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAACTT290.2015848741832337No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA280.19463367162519116No Hit
GCCACAACTGGGATTAGATACCCCCGCAACCTAGAGGAGCCTGTTCTAAC270.1876824690671486No Hit
GCCACAACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAACTTCG260.18073126650910606No Hit
GCCACAACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTAACTT260.18073126650910606No Hit
GCCACAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG250.17378006395106352No Hit
GCCACAACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTAAC250.17378006395106352No Hit
GCCACAACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAACTT240.166828861393021No Hit
GCCACAACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAACTTC230.15987765883497845No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA230.15987765883497845No Hit
GCCACAACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTAACTT220.1529264562769359No Hit
GCCACAACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTA210.14597525371889336No Hit
GCCACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG200.13902405116085081No Hit
GCCACAACTGGGATTAGATACCCCCCACGAGACCTAGAGGAGCCTGTTCT200.13902405116085081No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA190.13207284860280827No Hit
GCCACAACTGGGATTAGATACCCCGACGTGCCTAGAGGAGCCTGTTCTAA190.13207284860280827No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAACA190.13207284860280827No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT190.13207284860280827No Hit
GCCACAACTGGGATTAGATACCCCGAGATCGAAAGAGCGCTAGAGGAGCC180.12512164604476575No Hit
GCCACAACTGGGATTAGATACCCCGGCCCACGAGACCTAGAGGAGCCTGT180.12512164604476575No Hit
GCCACAACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTA180.12512164604476575No Hit
GCCACAACTGGGATTAGATACCCCACCAACCTAGAGGAGCCTGTTCTAAC170.1181704434867232No Hit
GCCACAACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTAACTT160.11121924092868066No Hit
GCCACAACTGGGATTAGATACCCCAAGCCCACGAGACCTAGAGGAGCCTG160.11121924092868066No Hit
GCCACAACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATAT150.10426803837063811No Hit
GCCACAACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAAC150.10426803837063811No Hit
GCCACAACTGGGATTAGATACCCCGAGCCACGAGACCTAGAGGAGCCTGT150.10426803837063811No Hit
GCCACAACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGC150.10426803837063811No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTAACA150.10426803837063811No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTAAC150.10426803837063811No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTGG108.384506E-4294.926854
CACTGGG108.384506E-4294.926855
AACTGGG13950.0294.926826
CAACTGG14100.0293.880985
CTGGGAT14000.0292.820228
ACTGGGA14000.0292.820227
TGGGATT13950.0292.812659
GCCACAA14400.0291.854681
CCACAAC14600.0287.856662
ACAACTG14550.0285.80544
CACAACT15100.0277.348423
CACACTG150.0028226625196.617873
ACTGGAT150.0028226625196.617877
AACTGGA200.0066739474147.463426
CCACACT200.0066739474147.463422
CAGCCCT150.00430808659.1916130-34
TAGCCGT1550.059.1916130-34
CGAGGGC150.00430808659.1916130-34
CTGGCCC351.3731551E-859.1916130-34
TCAGCCC150.00430808659.1916130-34
Produced by FastQC (version 0.11.5)