FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-48-5-3-8-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-48-5-3-8-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14017
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA279719.954341157166297No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCT181212.92715987729186No Hit
TGATTGACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGCTGAGA10697.626453592066776No Hit
TGATTGACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGCTG5914.216308767924662No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA4533.231789969322965No Hit
TGATTGACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGCT3322.368552471998288No Hit
TGATTGACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGCTGAG2862.040379539131055No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGA2351.676535635299993No Hit
TGATTGACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGCT2051.462509809517015No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGA1851.3198259256616967No Hit
TGATTGACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTC1841.3126917314689306No Hit
TGATTGACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCT1721.2270814011557396No Hit
TGATTGACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGCTGA1681.1985446243846758No Hit
TGATTGACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCT1571.1200684882642504No Hit
TGATTGACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCA1571.1200684882642504No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCC1511.0772633231076552No Hit
TGATTGACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAG1370.977384604408932No Hit
TGATTGACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTC1360.970250410216166No Hit
TGATTGACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTA1360.970250410216166No Hit
TGATTGACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGCTG1340.9559820218306342No Hit
TGATTGACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTA1340.9559820218306342No Hit
TGATTGACACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTA1130.8061639437825497No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAA1070.7633587786259541No Hit
TGATTGACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAG1070.7633587786259541No Hit
TGATTGACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTC990.7062852250838269No Hit
TGATTGACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGCT960.684882642505529No Hit
TGATTGACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTC850.6064065063851037No Hit
TGATTGACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTA790.5636013412285082No Hit
TGATTGACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGCT780.5564671470357423No Hit
TGATTGACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCT750.5350645644574445No Hit
TGATTGACACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTAG710.5065277876863808No Hit
TGATTGACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAA700.4993935934936149No Hit
TGATTGACACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTC670.4779910109153171No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAA670.4779910109153171No Hit
TGATTGACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGCTG630.4494542341442534No Hit
TGATTGACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGCTG620.4423200399514875No Hit
TGATTGACACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGCTG600.42805165156595565No Hit
TGATTGACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGCTGA590.42091745737318964No Hit
TGATTGACACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCT580.41378326318042374No Hit
TGATTGACACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAGC530.37811229221659415No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAA490.3495755154455304No Hit
TGATTGACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGA450.3210387386744667No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAA430.3067703502889349No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATC390.27823357351787115No Hit
TGATTGACACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTG380.2710993793251052No Hit
TGATTGACACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGC380.2710993793251052No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAA370.2639651851323393No Hit
TGATTGACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAA370.2639651851323393No Hit
TGATTGACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGT370.2639651851323393No Hit
TGATTGACACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAGC370.2639651851323393No Hit
TGATTGACACTGGGATTAGATACCCCACTAACACTAGAGGAGCCTGTTCT360.25683099093957334No Hit
TGATTGACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAA360.25683099093957334No Hit
TGATTGACACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAGCTG350.24969679674680745No Hit
TGATTGACACTGGGATTAGATACCCCGCGCTAGAGGAGCCTGTTCTAGCT350.24969679674680745No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGA330.2354284083612756No Hit
TGATTGACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAA320.22829421416850967No Hit
TGATTGACACTGGGATTAGATACCCCGCCAACTAGAGGAGCCTGTTCTAG320.22829421416850967No Hit
TGATTGACACTGGGATTAGATACCCCGTCAGACTAGAGGAGCCTGTTCTA290.20689163159021187No Hit
TGATTGACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGCTG280.19975743739744597No Hit
TGATTGACACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTA280.19975743739744597No Hit
TGATTGACACTGGGATTAGATACCCCACTGACACTAGAGGAGCCTGTTCT270.19262324320468005No Hit
TGATTGACACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCT250.17835485481914817No Hit
TGATTGACACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCT240.17122066062638225No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCTAGAGGAGCCTGTTCTAGC230.16408646643361632No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCCGCGAGACCTAGAGGAGCC230.16408646643361632No Hit
TGATTGACACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTA230.16408646643361632No Hit
TGATTGACACTGGGATTAGATACCCCGGTAGTCTAGAGGAGCCTGTTCTA210.14981807804808447No Hit
TGATTGACACTGGGATTAGATACCCCTGCTGCAACTAGAGGAGCCTGTTC210.14981807804808447No Hit
TGATTGACACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTA210.14981807804808447No Hit
TGATTGACACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAGC210.14981807804808447No Hit
TGATTGACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTT210.14981807804808447No Hit
TGATTGACACTGGGATTAGATACCCCCGCCACCTAGAGGAGCCTGTTCTA200.14268388385531855No Hit
TGATTGACACTGGGATTAGATACCCCCACGAACCTAGAGGAGCCTGTTCT200.14268388385531855No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTA200.14268388385531855No Hit
TGATTGACACTGGGATTAGATACCCCAATATGCTAGAGGAGCCTGTTCTA200.14268388385531855No Hit
TGATTGACACTGGGATTAGATACCCCGATCTAGAGGAGCCTGTTCTAGCT200.14268388385531855No Hit
TGATTGACACTGGGATTAGATACCCCGAGTCTAGAGGAGCCTGTTCTAGC190.1355496896625526No Hit
TGATTGACACTGGGATTAGATACCCCGACCCGACCTAGAGGAGCCTGTTC170.12128130127702075No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCCACGAGACTAGAGGAGCCT170.12128130127702075No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATC160.11414710708425484No Hit
TGATTGACACTGGGATTAGATACCCCAGTCTAGAGGAGCCTGTTCTAGCT160.11414710708425484No Hit
TGATTGACACTGGGATTAGATACCCCACTAGACCTAGAGGAGCCTGTTCT160.11414710708425484No Hit
TGATTGACACTGGGATTAGATACCCCACGATACCTAGAGGAGCCTGTTCT160.11414710708425484No Hit
TGATTGACACTGGGATTAGATACCCCCACGAACATAGAGGAGCCTGTTCT150.10701291289148891No Hit
TGATTGACACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCT150.10701291289148891No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTGAA157.0888873E-6295.02
TACTGGG108.3760894E-4295.08
ATACTGG108.3760894E-4295.07
CACTGGG13700.0293.923348
GATTGAC13650.0293.91942
TTGACAC13600.0293.915444
TGATTGA13950.0292.88531
ACTGGGA13900.0292.87779
ACACTGG13700.0292.84677
ATTGACA13750.0291.781833
GACACTG13900.0287.571936
TGACACT14000.0285.517855
ATTGAAC200.006667265147.53
TTGATAC401.246397E-473.754
TGATACT452.4906054E-465.555565
GCCCCAA150.004363367359.000004120-124
AATCTTG257.757957E-659.00000440-44
CCAACCT257.757957E-659.00000425-29
ACGCTAG150.004363367359.00000425-29
GCCTCAG501.8189894E-1259.000004120-124
Produced by FastQC (version 0.11.5)