FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-47-4-2-6-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-47-4-2-6-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14306
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA284019.85181042919055No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG13109.15699706416888No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGGAATCGCT12228.541870543827764No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA6774.732280162169719No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGGAATC5994.1870543827764575No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAG4192.9288410457150844No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA3452.4115755627009645No Hit
GCCACAACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA2962.0690619320564796No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGGAATCGC2541.7754788200754927No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGGAATCG2331.6286872640849992No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA2331.6286872640849992No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGGAAT2291.6007269677058578No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT2121.4818957080945059No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAG2111.4749056339997204No Hit
GCCACAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGGAATC1781.2442331888718021No Hit
GCCACAACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAGG1721.2022927443030895No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGGAAT1461.020550817838669No Hit
GCCACAACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGGA1461.020550817838669No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA1280.8947294841325318No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAG1270.8877394100377464No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA1270.8877394100377464No Hit
GCCACAACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGGAAT1250.8737592618481757No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC1190.8318188172794632No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA1180.8248287431846777No Hit
GCCACAACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGGAA900.6291066685306864No Hit
GCCACAACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC890.6221165944359011No Hit
GCCACAACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGGAATC890.6221165944359011No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA830.5801761498671886No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA700.48930518663497835No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA630.4403746679714805No Hit
GCCACAACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGGAATCG610.4263945197819097No Hit
GCCACAACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGG610.4263945197819097No Hit
GCCACAACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTA610.4263945197819097No Hit
GCCACAACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGGAAT590.41241437159233885No Hit
GCCACAACTGGGATTAGATACCCCCACACCTAGAGGAGCCTGTTCTAGGA580.4054242974975535No Hit
GCCACAACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAGGAATC550.38445407521319724No Hit
GCCACAACTGGGATTAGATACCCCATGTGTATAGAGGAGCCTGTTCTAGG530.37047392702362647No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA510.35649377883405564No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA500.3495037047392702No Hit
GCCACAACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAGG490.3425136306444848No Hit
GCCACAACTGGGATTAGATACCCCGGTAGCTAGAGGAGCCTGTTCTAGGA490.3425136306444848No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA440.3075632601705578No Hit
GCCACAACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTAGGAA410.2865930378862016No Hit
GCCACAACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAGGAATC410.2865930378862016No Hit
GCCACAACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT400.2796029637914162No Hit
GCCACAACTGGGATTAGATACCCCGCCAACTAGAGGAGCCTGTTCTAGGA380.26562281560184536No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA380.26562281560184536No Hit
GCCACAACTGGGATTAGATACCCCATAATCCTAGAGGAGCCTGTTCTAGG330.23067244512791837No Hit
GCCACAACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAGGAA330.23067244512791837No Hit
GCCACAACTGGGATTAGATACCCCCCACCTAGAGGAGCCTGTTCTAGGAA330.23067244512791837No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA330.23067244512791837No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA320.22368237103313296No Hit
GCCACAACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAGG310.21669229693834755No Hit
GCCACAACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAGG310.21669229693834755No Hit
GCCACAACTGGGATTAGATACCCCCACCCTAGAGGAGCCTGTTCTAGGAA280.19572207465399133No Hit
GCCACAACTGGGATTAGATACCCCACTAACACTAGAGGAGCCTGTTCTAG280.19572207465399133No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA270.18873200055920591No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA260.18174192646442053No Hit
GCCACAACTGGGATTAGATACCCCGCACGGACCTAGAGGAGCCTGTTCTA260.18174192646442053No Hit
GCCACAACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAGGAA260.18174192646442053No Hit
GCCACAACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGGAATC250.1747518523696351No Hit
GCCACAACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTAGGAA250.1747518523696351No Hit
GCCACAACTGGGATTAGATACCCCGCAACCTAGAGGAGCCTGTTCTAGGA250.1747518523696351No Hit
GCCACAACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTAG240.1677617782748497No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGTCTAGAGGAGCCTGTTCTAG240.1677617782748497No Hit
GCCACAACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGGAATC230.1607717041800643No Hit
GCCACAACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAG230.1607717041800643No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT230.1607717041800643No Hit
GCCACAACTGGGATTAGATACCCCACAACCTAGAGGAGCCTGTTCTAGGA220.1537816300852789No Hit
GCCACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG210.14679155599049348No Hit
GCCACAACTGGGATTAGATACCCCTGTCAGACTAGAGGAGCCTGTTCTAG210.14679155599049348No Hit
GCCACAACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAGG200.1398014818957081No Hit
GCCACAACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTT200.1398014818957081No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG200.1398014818957081No Hit
GCCACAACTGGGATTAGATACCCCCGCCTAGAGGAGCCTGTTCTAGGAAT190.13281140780092268No Hit
GCCACAACTGGGATTAGATACCCCACTAACCTAGAGGAGCCTGTTCTAGG180.1258213337061373No Hit
GCCACAACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAGGAAT180.1258213337061373No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT180.1258213337061373No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG170.11883125961135188No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA160.11184118551656648No Hit
GCCACAACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTAG160.11184118551656648No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTC150.10485111142178108No Hit
GCCACAACTGGGATTAGATACCCCAGGACCTAGAGGAGCCTGTTCTAGGA150.10485111142178108No Hit
GCCACAACTGGGATTAGATACCCCGTCAGACTAGAGGAGCCTGTTCTAGG150.10485111142178108No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTGG14050.0294.999975
AACTGGG14050.0293.950166
CTGGGAT14000.0293.946448
ACTGGGA14000.0293.946447
TGGGATT13900.0293.938849
GCCACAA14350.0290.88851
CCACAAC14750.0283.02
ACAACTG14700.0281.955784
CACAACT14900.0279.161073
TGGGATA150.0028204645196.666679
GGAGATC257.759656E-659.00000425-29
AAACCTT501.8189894E-1259.00000440-44
AATCTTG257.759656E-659.00000440-44
CCCAGTA501.8189894E-1259.00000420-24
CTTTACC257.759656E-659.00000455-59
GATACTT257.759656E-659.00000445-49
CCTAAAT257.759656E-659.00000435-39
CCGCCAG950.059.00000465-69
GATCGAG257.759656E-659.00000430-34
CCTTGAT501.8189894E-1259.00000440-44
Produced by FastQC (version 0.11.5)