FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-46-6-4-8-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-46-6-4-8-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13534
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA456033.69292153095906No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA12489.22122062952564No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTATAA9747.196689818235555No Hit
TGATTGACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGCTGAGA7555.578542928919758No Hit
TGATTGACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAA6564.847051869366041No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCT5814.292891975764741No Hit
TGATTGACACTGGGATTAGATACCCCATTATTCTCTTTTTTAATTTTTTT4513.332348160189153No Hit
TGATTGACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCC4363.2215161814688935No Hit
TGATTGACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAA3662.7043002807743464No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGA3202.364415546032215No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGA2782.054086005615487No Hit
TGATTGACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCA2691.9875868183833307No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGA2251.662479680803901No Hit
TGATTGACACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAGCTG1861.374316536131225No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGA1751.2930397517363676No Hit
TGATTGACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGCTG1641.2117629673415102No Hit
TGATTGACACTGGGATTAGATACCCCGATGTGTATAAGAGACAGTCTCAA1371.012265405645042No Hit
TGATTGACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCT900.6649918723215605No Hit
TGATTGACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGCT810.5984926850894045No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCC750.5541598936013004No Hit
TGATTGACACTGGGATTAGATACCCCTGACGCTGCTAGAGGAGCCTGTTC630.4654943106250924No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGA610.45071671346239106No Hit
TGATTGACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGCTGA600.44332791488104034No Hit
TGATTGACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGCTGA600.44332791488104034No Hit
TGATTGACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAG390.28816314467267623No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAA280.20688636027781881No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAA260.1921087631151175No Hit
TGATTGACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC230.16994236737106547No Hit
TGATTGACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGCTGAG230.16994236737106547No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGA190.14038717304566278No Hit
TGATTGACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAAC180.13299837446431212No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAA170.12560957588296143No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAA160.11822077730161075No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTGG13300.0295.07
CACTGGG13300.0295.08
GATTGAC13450.0295.02
ATTGACA13450.0295.03
ACTGGGA13300.0295.09
GACACTG13400.0293.899266
TGATTGA13550.0292.822881
TGACACT13500.0292.814825
TTGACAC13650.0290.677644
ATCATAA600.00177026273.74999295
CTTTACA900.059.000004120-124
CTGGGAT13300.059.00000410-14
CCTAAAG900.059.00000435-39
CATCCCT900.059.000004125-129
GCCAGAC900.059.00000470-74
ACTTAGC900.059.00000430-34
CGGTGCC1800.059.000004115-119
TACCCCT900.059.00000420-24
CTATGCC1800.059.00000425-29
AAAGTCG900.059.00000440-44
Produced by FastQC (version 0.11.5)