FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-45-4-4-1-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-45-4-4-1-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13752
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG246217.902850494473533No Hit
GGTACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAGA170212.376381617219314No Hit
GGTACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGATTGAGACT13499.809482257126236No Hit
GGTACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGATTGAG7505.453752181500873No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAGA5554.035776614310646No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG3942.8650378126817917No Hit
GGTACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGATTGAGA2591.8833624200116348No Hit
GGTACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGATTGAGAC2581.8760907504363002No Hit
GGTACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGATTGA2451.7815590459569517No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAGA2441.7742873763816174No Hit
GGTACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGATTGA2311.6797556719022686No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC2081.5125072716695753No Hit
GGTACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGATTGAG1931.403432228039558No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG1701.2361838278068644No Hit
GGTACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT1521.1052937754508436No Hit
GGTACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT1370.9962187318208261No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAGA1220.8871436881908087No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG1160.8435136707388016No Hit
GGTACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAGAT1100.7998836532867947No Hit
GGTACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAGAT790.5744618964514252No Hit
GGTACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGATT790.5744618964514252No Hit
GGTACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGATTGAG780.5671902268760907No Hit
GGTACACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTAGATT670.4872018615474113No Hit
GGTACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGATTGA660.4799301919720768No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG640.4653868528214078No Hit
GGTACACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTAGATTG630.45811518324607325No Hit
GGTACACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAGAT610.44357184409540434No Hit
GGTACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGATTGAG560.4072134962187318No Hit
GGTACACTGGGATTAGATACCCCGTAGTCCTAGAGGAGCCTGTTCTAGAT560.4072134962187318No Hit
GGTACACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTAGATTG560.4072134962187318No Hit
GGTACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGAT550.39994182664339734No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAG550.39994182664339734No Hit
GGTACACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGATTG520.37812681791739383No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT490.3563118091913903No Hit
GGTACACTGGGATTAGATACCCCTGACCTAGAGGAGCCTGTTCTAGATTG480.34904013961605584No Hit
GGTACACTGGGATTAGATACCCCCGTACGTGCAGGGAGGGTACCTTGAGG460.3344968004653869No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG460.3344968004653869No Hit
GGTACACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAGA440.31995346131471786No Hit
GGTACACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAGATTGAG440.31995346131471786No Hit
GGTACACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTAG430.3126817917393834No Hit
GGTACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG400.29086678301337987No Hit
GGTACACTGGGATTAGATACCCCCCGAGACCTAGAGGAGCCTGTTCTAGA390.28359511343804533No Hit
GGTACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG390.28359511343804533No Hit
GGTACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAG390.28359511343804533No Hit
GGTACACTGGGATTAGATACCCCTAGTTGACTAGAGGAGCCTGTTCTAGA390.28359511343804533No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAG380.2763234438627109No Hit
GGTACACTGGGATTAGATACCCCGGGTAGTCTAGAGGAGCCTGTTCTAGA370.26905177428737637No Hit
GGTACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGATTGAGA370.26905177428737637No Hit
GGTACACTGGGATTAGATACCCCTGAGCCCACGAGACCTAGAGGAGCCTG360.2617801047120419No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG360.2617801047120419No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG360.2617801047120419No Hit
GGTACACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCTG360.2617801047120419No Hit
GGTACACTGGGATTAGATACCCCTGACGCTAGAGGAGCCTGTTCTAGATT350.2545084351367074No Hit
GGTACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGATTGAG350.2545084351367074No Hit
GGTACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGATTGAG340.24723676556137286No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAG340.24723676556137286No Hit
GGTACACTGGGATTAGATACCCCGACCCACGAGACCTAGAGGAGCCTGTT340.24723676556137286No Hit
GGTACACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAGAT320.2326934264107039No Hit
GGTACACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTTC320.2326934264107039No Hit
GGTACACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTAG290.2108784176847004No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG290.2108784176847004No Hit
GGTACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT280.2036067481093659No Hit
GGTACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTCT270.1963350785340314No Hit
GGTACACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAGATTG270.1963350785340314No Hit
GGTACACTGGGATTAGATACCCCCGCCTAGAGGAGCCTGTTCTAGATTGA260.18906340895869692No Hit
GGTACACTGGGATTAGATACCCCACACTAGAGGAGCCTGTTCTAGATTGA240.17452006980802792No Hit
GGTACACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTAGA230.16724840023269344No Hit
GGTACACTGGGATTAGATACCCCCGACTCACTAGAGGAGCCTGTTCTAGA230.16724840023269344No Hit
GGTACACTGGGATTAGATACCCCGTGCTAGAGGAGCCTGTTCTAGATTGA230.16724840023269344No Hit
GGTACACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAGATTG230.16724840023269344No Hit
GGTACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG210.15270506108202442No Hit
GGTACACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTAGA200.14543339150668994No Hit
GGTACACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTAGATTGAGA200.14543339150668994No Hit
GGTACACTGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAGAT190.13816172193135545No Hit
GGTACACTGGGATTAGATACCCCGATCTAGAGGAGCCTGTTCTAGATTGA170.12361838278068643No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG170.12361838278068643No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTCG160.11634671320535195No Hit
GGTACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT160.11634671320535195No Hit
GGTACACTGGGATTAGATACCCCAAGAATCTGCTAGAGGAGCCTGTTCTA160.11634671320535195No Hit
GGTACACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATATC150.10907504363001745No Hit
GGTACACTGGGATTAGATACCCCTGACTAGAGGAGCCTGTTCTAGATTGA150.10907504363001745No Hit
GGTACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGATTGA140.10180337405468295No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGAT13500.0295.07
CTGGATT108.374456E-4295.07
ACTGGAT108.374456E-4295.06
GGGATAG157.086119E-6294.999979
TGGGATA157.086119E-6294.999978
CACTGGG13550.0293.911445
ACTGGGA13550.0293.911446
TGGGATT13400.0293.899268
GGGATTA13350.0293.895149
GGTACAC13800.0292.86231
ACACTGG13700.0292.84674
GTACACT13900.0290.75542
TACACTG13900.0289.694243
CACTGGA200.006665971147.55
TGGATTA200.006665971147.58
CCAGCTT452.5465852E-1159.00000485-89
GATATCC452.5465852E-1159.00000460-64
GCCTAGC452.5465852E-1159.00000425-29
CGCCAGG452.5465852E-1159.00000465-69
CGCCAGC452.5465852E-1159.00000485-89
Produced by FastQC (version 0.11.5)