FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-44-5-3-8-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-44-5-3-8-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13470
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA349925.976243504083147No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCT163612.14550853749072No Hit
TGATTGACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAACTTCGC7065.241276911655531No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA5954.417223459539718No Hit
TGATTGACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAACTT3862.8656273199703044No Hit
TGATTGACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCT2681.9896065330363772No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGA2611.9376391982182626No Hit
TGATTGACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTC2311.7149220489977728No Hit
TGATTGACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAACT2191.6258351893095768No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGA2171.6109873793615441No Hit
TGATTGACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAACTT2041.5144766146993318No Hit
TGATTGACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCA2001.4847809948032664No Hit
TGATTGACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCT1991.4773570898292503No Hit
TGATTGACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAACTTCG1921.4253897550111359No Hit
TGATTGACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTC1921.4253897550111359No Hit
TGATTGACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAACTTC1711.2694877505567927No Hit
TGATTGACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAA1701.2620638455827766No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAA1571.1655530809205643No Hit
TGATTGACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAACT1170.8685968819599109No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCC1170.8685968819599109No Hit
TGATTGACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCT1090.8092056421677802No Hit
TGATTGACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAACT910.6755753526354862No Hit
TGATTGACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAA900.6681514476614699No Hit
TGATTGACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTC840.623608017817372No Hit
TGATTGACACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTAACT750.5567928730512249No Hit
TGATTGACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAACTT670.4974016332590943No Hit
TGATTGACACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAACT670.4974016332590943No Hit
TGATTGACACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTA610.4528582034149963No Hit
TGATTGACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAACT610.4528582034149963No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAA610.4528582034149963No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAA570.42316258351893093No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAA530.39346696362286565No Hit
TGATTGACACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCT520.3860430586488493No Hit
TGATTGACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTC510.37861915367483295No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGA490.3637713437268003No Hit
TGATTGACACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGAC450.33407572383073497No Hit
TGATTGACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAA440.3266518188567187No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAA430.3192279138827023No Hit
TGATTGACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTA430.3192279138827023No Hit
TGATTGACACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTAA420.311804008908686No Hit
TGATTGACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAA410.30438010393466963No Hit
TGATTGACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTA400.2969561989606533No Hit
TGATTGACACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAAC390.289532293986637No Hit
TGATTGACACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAAC370.2746844840386043No Hit
TGATTGACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTT370.2746844840386043No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATC360.26726057906458794No Hit
TGATTGACACTGGGATTAGATACCCCCGTACGTGCAGGGAGGGTACCTTG340.2524127691165553No Hit
TGATTGACACTGGGATTAGATACCCCACGGGACCTAGAGGAGCCTGTTCT320.23756495916852266No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAA310.23014105419450634No Hit
TGATTGACACTGGGATTAGATACCCCCACCCTAGAGGAGCCTGTTCTAAC290.21529324424647364No Hit
TGATTGACACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAACTT280.20786933927245732No Hit
TGATTGACACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGC270.20044543429844097No Hit
TGATTGACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGA270.20044543429844097No Hit
TGATTGACACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAACTT250.1855976243504083No Hit
TGATTGACACTGGGATTAGATACCCCTGCTGCAACTAGAGGAGCCTGTTC230.17074981440237563No Hit
TGATTGACACTGGGATTAGATACCCCTGATCTGCTAGAGGAGCCTGTTCT230.17074981440237563No Hit
TGATTGACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAG230.17074981440237563No Hit
TGATTGACACTGGGATTAGATACCCCCGACCAACCTAGAGGAGCCTGTTC210.155902004454343No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATC200.14847809948032664No Hit
TGATTGACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAA200.14847809948032664No Hit
TGATTGACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAACTT200.14847809948032664No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAA190.14105419450631032No Hit
TGATTGACACTGGGATTAGATACCCCCCACCTAGAGGAGCCTGTTCTAAC190.14105419450631032No Hit
TGATTGACACTGGGATTAGATACCCCCAAACCTAGAGGAGCCTGTTCTAA180.13363028953229397No Hit
TGATTGACACTGGGATTAGATACCCCACGAGAGCTAGAGGAGCCTGTTCT180.13363028953229397No Hit
TGATTGACACTGGGATTAGATACCCCCGAGAAGACCTAGAGGAGCCTGTT170.12620638455827765No Hit
TGATTGACACTGGGATTAGATACCCCTAGTTGACTAGAGGAGCCTGTTCT170.12620638455827765No Hit
TGATTGACACTGGGATTAGATACCCCATGTGTATAGAGGAGCCTGTTCTA170.12620638455827765No Hit
TGATTGACACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTA170.12620638455827765No Hit
TGATTGACACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAAC160.11878247958426133No Hit
TGATTGACACTGGGATTAGATACCCCAAACCTAGAGGAGCCTGTTCTAAC160.11878247958426133No Hit
TGATTGACACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAAC160.11878247958426133No Hit
TGATTGACACTGGGATTAGATACCCCCGCCTAGAGGAGCCTGTTCTAACT150.11135857461024498No Hit
TGATTGACACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAA150.11135857461024498No Hit
TGATTGACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC150.11135857461024498No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGA150.11135857461024498No Hit
TGATTGACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCC140.10393466963622866No Hit
TGATTGACACTGGGATTAGATACCCCCACACCTAGAGGAGCCTGTTCTAA140.10393466963622866No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT157.085153E-6294.9749
CACGGGA157.085153E-6294.9748
TGATTGA13400.0293.873351
GATTGAC13400.0292.77272
TTGACAC13350.0292.764434
GACACTG13200.0292.739326
CACTGGG13150.0292.730838
ACTGGGA13150.0292.730839
ACACTGG13300.0291.647227
ATTGACA13500.0290.603973
TGACACT13350.0289.45015
ACACGGG202.2332853E-5221.23057
GACACGG202.2332853E-5221.23056
CACTGGA150.0028193423196.649348
TGACACG255.4378164E-5176.984385
GCCCCAA201.8357324E-458.9948120-124
AACGCTT201.8357324E-458.994890-94
ACGCTTA201.8357324E-458.994890-94
CCCGGTA201.8357324E-458.994820-24
GATATCC800.058.994865-69
Produced by FastQC (version 0.11.5)