FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-43-6-3-8-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-43-6-3-8-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13293
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA719154.09614082599865No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA6064.558790340780862No Hit
TGATTGACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCA4503.3852403520649963No Hit
TGATTGACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAA4303.2347852253065525No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAA2742.061235236590687No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGA2351.7678477394117205No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCATAAACTTAAA2261.7001429323704205No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAA2241.685097419694576No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAGCCTTGA1921.4443692168810651No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAG1671.2563003084330098No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAA1601.2036410140675544No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGA1581.1885955013917098No Hit
TGATTGACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAA1541.1585044760400212No Hit
TGATTGACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCACTCC1471.1058451816745656No Hit
TGATTGACACTGGGATTAGATACCCCCTATGCTTAGCCCTAAACCTCAAC1351.0155721056194988No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAGCCTCAA1341.0080493492815767No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAA1280.9629128112540434No Hit
TGATTGACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTC1180.8876852478748214No Hit
TGATTGACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACCAC1090.8199804408335214No Hit
TGATTGACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACCA1050.7898894154818324No Hit
TGATTGACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCT1020.7673211464680658No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCATAAACCTAAA1000.7522756337922215No Hit
TGATTGACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACCAC840.631911532385466No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATC740.556683969006244No Hit
TGATTGACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGA700.526592943654555No Hit
TGATTGACACTGGGATTAGATACCCCGTCTTTATACACATCTGAGCTAGA700.526592943654555No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGA600.45136538027533285No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCT500.37613781689611075No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGA500.37613781689611075No Hit
TGATTGACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC430.32347852253065523No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATC410.3084330098548108No Hit
TGATTGACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAC400.3009102535168886No Hit
TGATTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC270.2031144211238998No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAA230.17302339577221093No Hit
TGATTGACACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGAT220.16550063943428872No Hit
TGATTGACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAAC210.1579778830963665No Hit
TGATTGACACTGGGTTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC180.13540961408259986No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGA150.11284134506883321No Hit
TGATTGACACTGGGATTAGATACCCAACTATGCTTAGCCCTAAACTATAA150.11284134506883321No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAA140.105318588730911No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTGG13100.0295.07
CACTGGG13050.0295.08
GACACTG13100.0295.06
ATTGACA13150.0295.03
ACTGGGA13100.0295.09
CGATAAA157.080267E-6294.99997295
GATTGAC13200.0293.882572
TGATTGA13300.0292.781951
TTGACAC13250.0292.77364
TGACACT13350.0289.475655
CACTGGA150.002818125196.666668
ACTGGAT150.002818125196.666669
CTTCATA8600.059.000004120-124
CAGTTTA452.5465852E-1159.00000490-94
TAAACTT1050.059.00000440-44
GATACCC13250.059.00000415-19
CGCCAGA9450.059.00000470-74
GGATTAG13250.059.00000410-14
ACCTGGC8500.059.000004110-114
CTGGCGG8600.059.000004110-114
Produced by FastQC (version 0.11.5)