FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-42-4-5-7-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-42-4-5-7-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13524
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC210015.527950310559005No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA184413.635019225081338No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGATTGAGA12509.242827565808932No Hit
CTGGATGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGATTG7805.767524401064774No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA4973.674948240165631No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT3532.6101745045844424No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT3192.3587695947944396No Hit
CTGGATGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGATT2912.1517302573203194No Hit
CTGGATGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT2631.9446909198461995No Hit
CTGGATGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGATTGAG2521.8633540372670807No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA2261.671103223898255No Hit
CTGGATGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGATTG1831.3531499556344277No Hit
CTGGATGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC1811.3383614315291332No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGATTGA1701.2570245489500147No Hit
CTGGATGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGATT1641.2126589766341318No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT1481.0943507837917774No Hit
CTGGATGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGATT1371.0130139012126589No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT1340.9908311150547175No Hit
CTGGATGACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAGAT1280.9464655427388345No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC1160.857734398107069No Hit
CTGGATGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGATT1160.857734398107069No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT1120.8281573498964804No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT1080.7985803016858917No Hit
CTGGATGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGATTG950.7024548950014788No Hit
CTGGATGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAG890.658089322685596No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAG880.6506950606329488No Hit
CTGGATGACTGGGATTAGATACCCCACGACCTAGAGGAGCCTGTTCTAGA860.6359065365276546No Hit
CTGGATGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAG830.6137237503697132No Hit
CTGGATGACTGGGATTAGATACCCCTGACGCTACTAGAGGAGCCTGTTCT830.6137237503697132No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA780.5767524401064774No Hit
CTGGATGACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCC710.5249926057379474No Hit
CTGGATGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGATTG710.5249926057379474No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT690.5102040816326531No Hit
CTGGATGACTGGGATTAGATACCCCATGTCAGACTAGAGGAGCCTGTTCT650.4806270334220645No Hit
CTGGATGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGA640.47323277136941727No Hit
CTGGATGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT610.45104998521147593No Hit
CTGGATGACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTC590.4362614611061816No Hit
CTGGATGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGATTG560.4140786749482402No Hit
CTGGATGACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTAGA520.3845016267376516No Hit
CTGGATGACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTAGAT460.3401360544217687No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC420.3105590062111801No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT410.30316474415853295No Hit
CTGGATGACTGGGATTAGATACCCCCGTGGACTAGAGGAGCCTGTTCTAG390.2883762200532387No Hit
CTGGATGACTGGGATTAGATACCCCACCACCTAGAGGAGCCTGTTCTAGA380.28098195800059156No Hit
CTGGATGACTGGGATTAGATACCCCAAGGAGATCGAGGTCCTAGAGGAGC380.28098195800059156No Hit
CTGGATGACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAGATTG340.25140490979000296No Hit
CTGGATGACTGGGATTAGATACCCCGTCAGACTAGAGGAGCCTGTTCTAG340.25140490979000296No Hit
CTGGATGACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAGAT320.23661638568470864No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT320.23661638568470864No Hit
CTGGATGACTGGGATTAGATACCCCTGGACCTAGAGGAGCCTGTTCTAGA320.23661638568470864No Hit
CTGGATGACTGGGATTAGATACCCCGACGTGCCTAGAGGAGCCTGTTCTA310.22922212363206151No Hit
CTGGATGACTGGGATTAGATACCCCTCCAAGCCCACGAGACCTAGAGGAG260.1922508133688258No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC250.18485655131617865No Hit
CTGGATGACTGGGATTAGATACCCCCCACGAGACCTAGAGGAGCCTGTTC240.1774622892635315No Hit
CTGGATGACTGGGATTAGATACCCCCACACCTAGAGGAGCCTGTTCTAGA230.17006802721088435No Hit
CTGGATGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA220.1626737651582372No Hit
CTGGATGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAG220.1626737651582372No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT210.15527950310559005No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT200.14788524105294293No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGTCTAGAGGAGCCTGTTCTA180.13309671694764863No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCT180.13309671694764863No Hit
CTGGATGACTGGGATTAGATACCCCCGAGAAGACCTAGAGGAGCCTGTTC170.12570245489500148No Hit
CTGGATGACTGGGATTAGATACCCCTATAAAGGGCTAGAGGAGCCTGTTC170.12570245489500148No Hit
CTGGATGACTGGGATTAGATACCCCCGGACCTAGAGGAGCCTGTTCTAGA170.12570245489500148No Hit
CTGGATGACTGGGATTAGATACCCCTGTGGACTAGAGGAGCCTGTTCTAG170.12570245489500148No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT170.12570245489500148No Hit
CTGGATGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGATTG160.11830819284235432No Hit
CTGGATGACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATA160.11830819284235432No Hit
CTGGATGACTGGGATTAGATACCCCCACGAACCTAGAGGAGCCTGTTCTA150.11091393078970718No Hit
CTGGATGACTGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG140.10351966873706005No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATGGG108.372762E-4295.06
GATGGGA108.372762E-4295.07
ATGGGAT108.372762E-4295.08
GGATGAC13200.0295.03
TGACTGG13100.0295.06
GATGATG108.372762E-4295.04
CTGGATT108.372762E-4295.09
CTGGATG13400.0295.01
TGGATGA13350.0295.02
GGATGAT157.0832466E-6294.999973
GACTGGG13050.0293.869727
GATGACT13250.0292.77364
ACTGGGA13150.0291.634988
CTGGGAT13200.0290.53039
ATGACTG13400.0289.496285
TCCAAAA150.0028187148196.66666295
ATGATGG200.00666463147.55
GACTGGA200.00666463147.57
ACTGGAT200.00666463147.58
AGTAACA650.00243179668.07693295
Produced by FastQC (version 0.11.5)