FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-41-4-3-8-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-41-4-3-8-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13503
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATTGACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCT213015.774272383914687No Hit
TGATTGACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGCTGAGA140910.434718210767977No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA138810.279197215433607No Hit
TGATTGACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGCTG6634.910019995556543No Hit
TGATTGACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGCTGAG3832.836406724431608No Hit
TGATTGACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGCT3702.74013182255795No Hit
TGATTGACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGCT2742.0291787010294007No Hit
TGATTGACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCT2291.6959194253128935No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA2271.6811079019477153No Hit
TGATTGACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCT2131.5774272383914687No Hit
TGATTGACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTC2081.5403984299785232No Hit
TGATTGACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGCTGA1981.4663408131526328No Hit
TGATTGACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTC1861.3774716729615641No Hit
TGATTGACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAG1841.3626601495963861No Hit
TGATTGACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTA1671.236762200992372No Hit
TGATTGACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTA1581.1701103458490707No Hit
TGATTGACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGCTG1431.0590239206102348No Hit
TGATTGACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTC1401.0368066355624677No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGA1330.9849663037843441No Hit
TGATTGACACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTA1320.9775605421017552No Hit
TGATTGACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAG1300.9627490187365771No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGA1170.8664741168629194No Hit
TGATTGACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGCT1070.7924165000370288No Hit
TGATTGACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCA1070.7924165000370288No Hit
TGATTGACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCT1030.7627934533066726No Hit
TGATTGACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTC1030.7627934533066726No Hit
TGATTGACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTA920.6813300747981931No Hit
TGATTGACACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTAG810.5998666962897135No Hit
TGATTGACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGCT800.5924609346071243No Hit
TGATTGACACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAGC790.5850551729245353No Hit
TGATTGACACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCT770.5702436495593572No Hit
TGATTGACACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTC770.5702436495593572No Hit
TGATTGACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGCTG740.54802636451159No Hit
TGATTGACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGCTG630.46656298600311047No Hit
TGATTGACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGCTGA600.4443457009553432No Hit
TGATTGACACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGCTG600.4443457009553432No Hit
TGATTGACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGT570.4221284159075761No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAA550.407316892542398No Hit
TGATTGACACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAGC490.36288232244686364No Hit
TGATTGACACTGGGATTAGATACCCCGCGCTAGAGGAGCCTGTTCTAGCT480.3554765607642746No Hit
TGATTGACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAA460.34066503739909654No Hit
TGATTGACACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCT460.34066503739909654No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATC450.33325927571650743No Hit
TGATTGACACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTG430.3184477523513293No Hit
TGATTGACACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAGCTG430.3184477523513293No Hit
TGATTGACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGCTG410.3036362289861512No Hit
TGATTGACACTGGGATTAGATACCCCGTCAGACTAGAGGAGCCTGTTCTA400.2962304673035622No Hit
TGATTGACACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGC390.2888247056209731No Hit
TGATTGACACTGGGATTAGATACCCCAATATGCTAGAGGAGCCTGTTCTA370.274013182255795No Hit
TGATTGACACTGGGATTAGATACCCCACTAACACTAGAGGAGCCTGTTCT360.26660742057320597No Hit
TGATTGACACTGGGATTAGATACCCCTGCTGCAACTAGAGGAGCCTGTTC360.26660742057320597No Hit
TGATTGACACTGGGATTAGATACCCCACTGACACTAGAGGAGCCTGTTCT350.2592016588906169No Hit
TGATTGACACTGGGATTAGATACCCCGGTAGTCTAGAGGAGCCTGTTCTA340.25179589720802786No Hit
TGATTGACACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAGC340.25179589720802786No Hit
TGATTGACACTGGGATTAGATACCCCGCCAACTAGAGGAGCCTGTTCTAG340.25179589720802786No Hit
TGATTGACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTT330.2443901355254388No Hit
TGATTGACACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTA310.22957861216026065No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAA280.20736132711249353No Hit
TGATTGACACTGGGATTAGATACCCCCACGAACCTAGAGGAGCCTGTTCT280.20736132711249353No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAA280.20736132711249353No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCTAGAGGAGCCTGTTCTAGC270.19995556542990447No Hit
TGATTGACACTGGGATTAGATACCCCTGACCTGCTAGAGGAGCCTGTTCT270.19995556542990447No Hit
TGATTGACACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCT270.19995556542990447No Hit
TGATTGACACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTA260.1925498037473154No Hit
TGATTGACACTGGGATTAGATACCCCGAGTCTAGAGGAGCCTGTTCTAGC260.1925498037473154No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTA250.18514404206472634No Hit
TGATTGACACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCT240.1777382803821373No Hit
TGATTGACACTGGGATTAGATACCCCCGCCACCTAGAGGAGCCTGTTCTA230.17033251869954827No Hit
TGATTGACACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAGC230.17033251869954827No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAA230.17033251869954827No Hit
TGATTGACACTGGGATTAGATACCCCGATCTAGAGGAGCCTGTTCTAGCT230.17033251869954827No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAA220.1629267570169592No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCC210.15552099533437014No Hit
TGATTGACACTGGGATTAGATACCCCCCTAACACTAGAGGAGCCTGTTCT210.15552099533437014No Hit
TGATTGACACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTAGCTGA200.1481152336517811No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATC200.1481152336517811No Hit
TGATTGACACTGGGATTAGATACCCCACTAGACCTAGAGGAGCCTGTTCT200.1481152336517811No Hit
TGATTGACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAA200.1481152336517811No Hit
TGATTGACACTGGGATTAGATACCCCAGTCTAGAGGAGCCTGTTCTAGCT190.14070947196919203No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGA190.14070947196919203No Hit
TGATTGACACTGGGATTAGATACCCCACGATACCTAGAGGAGCCTGTTCT190.14070947196919203No Hit
TGATTGACACTGGGATTAGATACCCCACCAACTAGAGGAGCCTGTTCTAG190.14070947196919203No Hit
TGATTGACACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGAT180.13330371028660298No Hit
TGATTGACACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTA180.13330371028660298No Hit
TGATTGACACTGGGATTAGATACCCCCGAGATCTAGAGGAGCCTGTTCTA180.13330371028660298No Hit
TGATTGACACTGGGATTAGATACCCCGACGACTAGAGGAGCCTGTTCTAG180.13330371028660298No Hit
TGATTGACACTGGGATTAGATACCCCAGGACCTAGAGGAGCCTGTTCTAG170.12589794860401393No Hit
TGATTGACACTGGGATTAGATACCCCTAGCCCACTAGAGGAGCCTGTTCT160.11849218692142488No Hit
TGATTGACACTGGGATTAGATACCCCGACCCGACCTAGAGGAGCCTGTTC150.1110864252388358No Hit
TGATTGACACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTAGC150.1110864252388358No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCACGAGACCTAGAGGAGCCT150.1110864252388358No Hit
TGATTGACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAA140.10368066355624676No Hit
TGATTGACACTGGGATTAGATACCCCTGACCGACGACTAGAGGAGCCTGT140.10368066355624676No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCCGCGAGACCTAGAGGAGCC140.10368066355624676No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTGGG13300.0295.08
GATTGAC13500.0295.02
ACTGGGA13300.0295.09
TTGACAC13500.0293.90744
ACACTGG13450.0292.80677
ATTGACA13600.0291.74633
GACACTG13600.0290.661776
TGATTGA13800.0288.586981
TGACACT13700.0288.540135
ACTACGT150.0028187148196.66666295
GATACCC13400.059.00000415-19
CTGGGAT13350.059.00000410-14
GGATTAG13400.059.00000410-14
GCCAGAA1800.059.00000470-74
CCCCGTC900.059.00000420-24
TACGAGC1800.059.00000480-84
GGGATTA13400.059.00000410-14
GCCACAG1800.059.00000485-89
GATTAGA13450.059.00000410-14
TGGGATT13350.059.00000410-14
Produced by FastQC (version 0.11.5)