FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-40-6-5-7-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-40-6-5-7-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13275
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC608945.868173258003765No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT7715.807909604519774No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGAT5083.8267419962335216No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT4253.2015065913371No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC3182.3954802259887007No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAATAGGATT3152.3728813559322033No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT3092.327683615819209No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT3062.305084745762712No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAAC1851.393596986817326No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC1771.3333333333333335No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGAT1541.160075329566855No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT1511.137476459510358No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT1351.0169491525423728No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT1250.9416195856873822No Hit
CTGGATGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT1170.8813559322033898No Hit
CTGGATGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAATAGGAT1130.8512241054613935No Hit
CTGGATGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT910.6854990583804144No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC870.6553672316384181No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT780.5875706214689265No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCT780.5875706214689265No Hit
CTGGATGACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATA630.47457627118644063No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT520.391713747645951No Hit
CTGGATGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCA450.3389830508474576No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAAT450.3389830508474576No Hit
CTGGATGACTGGGATTAGATACCCCACTATACTTAGCCCTAAACCTCAAC390.29378531073446323No Hit
CTGGATGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA380.2862523540489642No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGAC370.2787193973634651No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA370.2787193973634651No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAAT360.2711864406779661No Hit
CTGGATGACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTAATA360.2711864406779661No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGAT350.263653483992467No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAAC320.24105461393596986No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAGAT310.2335216572504708No Hit
CTGGATGACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTAATAGG310.2335216572504708No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATAT310.2335216572504708No Hit
CTGGATGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACA290.2184557438794727No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAAT290.2184557438794727No Hit
CTGGATGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAATAGG250.18832391713747645No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACACCATCCAATCT240.1807909604519774No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGGT210.1581920903954802No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAA200.15065913370998116No Hit
CTGGATGACTGGGATTAGATACCCCTCCGAGCCCACGAGACACGACTTGA200.15065913370998116No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAAC200.15065913370998116No Hit
CTGGATGACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGT200.15065913370998116No Hit
CTGGATGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAATAGG180.13559322033898305No Hit
CTGGATGACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAATAGGA180.13559322033898305No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAAC180.13559322033898305No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATAACT180.13559322033898305No Hit
CTGGATGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG180.13559322033898305No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACATCGAT170.128060263653484No Hit
CTGGATGACTGGGATTAGATACCCCCGAGCCCACGAGACTCTCCGATATC170.128060263653484No Hit
CTGGATGACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAAT170.128060263653484No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGT160.12052730696798493No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGAC160.12052730696798493No Hit
CTGGATGACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTAATAGGA160.12052730696798493No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACTCTAGAGGAGCCTGTTCT150.11299435028248588No Hit
CTGGATGATGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA150.11299435028248588No Hit
CTGGATGACTGGGATTAGATACCCCCCCCTAGAGGAGCCTGTTCTAATAG140.10546139359698682No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT140.10546139359698682No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT140.10546139359698682No Hit
CTGGATGACTGGGATTAGATACCCCGACGACGAGGAGCCTGTTCTAATAG140.10546139359698682No Hit
CTGGATGACTGGGATTGATACCCCACTATGCTTAGCCCTAAACCTCAACA140.10546139359698682No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTTAAC140.10546139359698682No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGAC13050.0295.03
ATGACTG13000.0295.05
TGACTGG13000.0295.06
GATGACT13050.0295.04
CTGGATG13050.0295.01
CTGGACG108.371005E-4295.01
TGGACGA108.371005E-4295.02
TGGATGA13050.0295.02
GACGACT108.371005E-4295.04
GACTGGA205.9584636E-8295.07
ACGACTG108.371005E-4295.05
CGACTGG108.371005E-4295.06
GGACGAC108.371005E-4295.03
CTGGGGT157.080267E-6294.999979
ACTGGGG157.080267E-6294.999978
ACTGGAT157.080267E-6294.999978
GACTGGG12950.0293.8617
CTGGGAT12800.0292.69539
ACTGGGA12800.0292.69538
CTGGATT255.4340744E-5177.09
Produced by FastQC (version 0.11.5)