FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-4-5-4-1-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-4-5-4-1-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10950
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG371133.89041095890411No Hit
GGTACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAAG7717.041095890410959No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG6275.726027397260274No Hit
GGTACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAAGTCTCCTGT5464.986301369863014No Hit
GGTACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAAGTCTCC3683.3607305936073057No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG2782.538812785388128No Hit
GGTACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAAGTCTC2502.28310502283105No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC2282.0821917808219177No Hit
GGTACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT1951.7808219178082192No Hit
GGTACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAAGTCTCCTG1871.7077625570776256No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG1601.461187214611872No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAAG1431.3059360730593608No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT1111.0136986301369864No Hit
GGTACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAAGTCTCC1010.9223744292237444No Hit
GGTACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAAGTCTCCT1000.91324200913242No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAAG960.8767123287671232No Hit
GGTACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT910.8310502283105023No Hit
GGTACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG880.8036529680365296No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG810.7397260273972602No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAA700.6392694063926941No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG660.6027397260273972No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG620.5662100456621004No Hit
GGTACACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAAGTCT560.5114155251141552No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG510.4657534246575342No Hit
GGTACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG500.45662100456621No Hit
GGTACACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCTG490.4474885844748858No Hit
GGTACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAAGTCTC470.4292237442922374No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG440.4018264840182648No Hit
GGTACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG410.3744292237442922No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG390.3561643835616438No Hit
GGTACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAAGTCTCC380.3470319634703196No Hit
GGTACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAAGTCTC380.3470319634703196No Hit
GGTACACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAAGTCTC360.32876712328767127No Hit
GGTACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCTA350.31963470319634707No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAA330.3013698630136986No Hit
GGTACACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAAGTCT310.2831050228310502No Hit
GGTACACTGGGATTAGATACCCCGAGCCACGAGACCTAGAGGAGCCTGTT310.2831050228310502No Hit
GGTACACTGGGATTAGATACCCCAATGAGATCGAGGGCCTAGAGGAGCCT310.2831050228310502No Hit
GGTACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAAGT310.2831050228310502No Hit
GGTACACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAAG290.2648401826484018No Hit
GGTACACTGGGATTAGATACCCCCACGAGATCTAGAGGAGCCTGTTCTAA260.2374429223744292No Hit
GGTACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTCT250.228310502283105No Hit
GGTACACTGGGATTAGATACCCCCTGTAGTCCTAGAGGAGCCTGTTCTAA250.228310502283105No Hit
GGTACACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCCTA250.228310502283105No Hit
GGTACACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAAGTCT240.2191780821917808No Hit
GGTACACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTTC240.2191780821917808No Hit
GGTACACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTAA220.2009132420091324No Hit
GGTACACTGGGATTAGATACCCCGTGTAGTCCCTAGAGGAGCCTGTTCTA220.2009132420091324No Hit
GGTACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT210.1917808219178082No Hit
GGTACACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTAAGTCT210.1917808219178082No Hit
GGTACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT210.1917808219178082No Hit
GGTACACTGGGATTAGATACCCCTATAAAGGGCTAGAGGAGCCTGTTCTA200.182648401826484No Hit
GGTACACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAAGTCTCC190.1735159817351598No Hit
GGTACACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTAAGTCTC190.1735159817351598No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG190.1735159817351598No Hit
GGTACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT180.16438356164383564No Hit
GGTACACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAAGTCT180.16438356164383564No Hit
GGTACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAAGT180.16438356164383564No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCTAGAGGAGCCTGTTCTAAGT170.15525114155251143No Hit
GGTACACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAAGTCT170.15525114155251143No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTCG160.1461187214611872No Hit
GGTACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT160.1461187214611872No Hit
GGTACACTGGGATTAGATACCCCAATGTCTAGAGGAGCCTGTTCTAAGTC160.1461187214611872No Hit
GGTACACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAAGTCTCC160.1461187214611872No Hit
GGTACACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCTAA160.1461187214611872No Hit
GGTACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAAGTC150.136986301369863No Hit
GGTACACTGGGATTAGATACCCCTCAGAGCCCACGAGACCTAGAGGAGCC150.136986301369863No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG140.1278538812785388No Hit
GGTACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATAA130.1187214611872146No Hit
GGTACACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTAAG130.1187214611872146No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG120.1095890410958904No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTAACAG120.1095890410958904No Hit
GGTACACTGGGATTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT120.1095890410958904No Hit
GGTACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAA110.1004566210045662No Hit
GGTACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGCC110.1004566210045662No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTCAACAG110.1004566210045662No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGAT10700.0294.899577
CACTGGG10750.0294.899575
ACTGGGA10750.0294.899576
ACACGGG108.3596085E-4294.899544
ACACTGG10800.0294.899544
CGGGATT108.3596085E-4294.899547
CACGGGA108.3596085E-4294.899545
TGGGATT10750.0293.527928
GGTACAC11000.0292.218661
TACACTG10900.0292.194033
GGGATTA10850.0290.822579
GTACACT11000.0289.537752
GTACACG150.0028143025196.59972
TACACGG150.0028143025196.59973
ACGGGAT150.0028143025196.59976
ATCATAA650.002422683968.05374295
AAACCTC4050.059.2504635-39
CCCAGTA201.7938597E-459.2504620-24
TAAACCC201.7938597E-459.2504635-39
AAATCTT201.7938597E-459.2504635-39
Produced by FastQC (version 0.11.5)