FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-39-6-4-8-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-39-6-4-8-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13051
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA398930.56470768523485No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA195414.972032794421883No Hit
TGATTGACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGATTGAG6565.026434755957398No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGA5954.559037621638189No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCT5133.930733277143514No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTTGG4813.685541337828519No Hit
TGATTGACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCA3822.926978775572753No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCATAAACATTGA3772.8886675350547852No Hit
TGATTGACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCC3412.6128266033254155No Hit
TGATTGACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGATT2692.061144739866677No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTCGA2551.9538732664163665No Hit
TGATTGACACTGGGATTAGATACCCCTGTAGTCCTAGCCGTAAACGATGG2451.8772507853804306No Hit
TGATTGACACTGGGATTAGATACCCCACTATGTCTAGCCCTAAACATTGA2301.7623170638265266No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACATTGA2291.7546548157229331No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCGTAAACATTGA1901.4558271396827829No Hit
TGATTGACACTGGGATTAGATACCCCTTCCTAGAGGAGCCTGTTCTAGAT1741.3332311700252852No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAA1721.3179066738180982No Hit
TGATTGACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAA1661.2719331851965368No Hit
TGATTGACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGAT1210.9271320205348248No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAGCCATAAACATTGA1130.8658340357060762No Hit
TGATTGACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGATTGA990.7585625622557659No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAA770.5899931039767068No Hit
TGATTGACACTGGGATTAGATACCCCTCGTCGGCAGCGTCAGATGTGTAT700.5363573672515516No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAA670.5133706229407708No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAGACCTCAA420.321814420350931No Hit
TGATTGACACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTAGA410.3141521722473374No Hit
TGATTGACACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTAGATTG390.2988276760401502No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAA310.23752969121140144No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTCAA250.19155620258983985No Hit
TGATTGACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC240.18389395448624626No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAA210.1609072101754655No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTATAA190.1455827139682783No Hit
TGATTGACACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC140.10727147345031034No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACACTG12800.0295.06
GATTGAC12850.0295.02
TTGACAC12850.0295.04
ACTGGAT108.369551E-4295.09
ACACTGG12900.0293.85667
CACTGGG12850.0293.852148
ACTGGGA12900.0292.713179
TGATTGA13100.0291.622131
TGACACT13050.0290.47895
ATTGACA13600.0278.731633
ACTGCTC3600.059.00000465-69
CGTCAGA150.0043620459.00000435-39
AGGGGAC150.0043620459.00000475-79
CGATAGA303.2936077E-759.00000445-49
TCTAGCC150.0043620459.00000430-34
CTTTACA150.0043620459.000004120-124
TCGATAG303.2936077E-759.00000445-49
AACTGCT3600.059.00000465-69
TGTCCCT150.0043620459.000004160-164
TATGCTT4800.059.00000425-29
Produced by FastQC (version 0.11.5)