FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-38-5-4-1-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-38-5-4-1-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13097
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG394030.083225166068566No Hit
GGTACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAGG11788.994426204474307No Hit
GGTACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGGAATCGCTG8756.680919294494922No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG6314.817897228372909No Hit
GGTACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGGAATCG5924.52011911124685No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG2882.1989768649309003No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC2642.0157287928533254No Hit
GGTACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT2071.5805146216690844No Hit
GGTACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGGAATCGCT1841.404901885928075No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAGG1691.2903718408795906No Hit
GGTACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGGAATC1661.267465831869894No Hit
GGTACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGGAATCG1561.1911124685042376No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAGG1551.1834771321676718No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG1491.137665114148278No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT1200.916240360387875No Hit
GGTACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGGAATCGC1040.7940749790028252No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG860.6566389249446438No Hit
GGTACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGGAATCG760.5802855615789876No Hit
GGTACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGGAA740.5650148889058563No Hit
GGTACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGGAATC740.5650148889058563No Hit
GGTACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG630.4810261892036344No Hit
GGTACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAGGA630.4810261892036344No Hit
GGTACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAG610.4657555165305032No Hit
GGTACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGGAATC600.4581201801939375No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG580.4428495075208063No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG540.4123081621745438No Hit
GGTACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGGAATCG540.4123081621745438No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAG530.40467282583797815No Hit
GGTACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAGGA530.40467282583797815No Hit
GGTACACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAGGA510.38940215316484694No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAG470.3588608078185844No Hit
GGTACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGGAATC450.34359013514545317No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG430.3283194624723219No Hit
GGTACACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTAGGAA420.3206841261357563No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG410.31304878979919065No Hit
GGTACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT400.30541345346262505No Hit
GGTACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG400.30541345346262505No Hit
GGTACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCTA390.2977781171260594No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAG380.2901427807894938No Hit
GGTACACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTAGG380.2901427807894938No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAGG380.2901427807894938No Hit
GGTACACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAGG370.28250744445292814No Hit
GGTACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTCT350.26723677177979693No Hit
GGTACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGGA340.2596014354432313No Hit
GGTACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG320.24433076277010002No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG310.23669542643353442No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG300.22906009009696876No Hit
GGTACACTGGGATTAGATACCCCAAACCTAGAGGAGCCTGTTCTAGGAAT290.22142475376040316No Hit
GGTACACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTAGG280.2137894174238375No Hit
GGTACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGGAATCG260.1985187447507063No Hit
GGTACACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTAG260.1985187447507063No Hit
GGTACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT250.19088340841414064No Hit
GGTACACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTAGGAATCGC250.19088340841414064No Hit
GGTACACTGGGATTAGATACCCCACGAGGCCTAGAGGAGCCTGTTCTAGG240.183248072077575No Hit
GGTACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGCC240.183248072077575No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTAGGA230.17561273574100938No Hit
GGTACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT230.17561273574100938No Hit
GGTACACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTAGGAA220.16797739940444378No Hit
GGTACACTGGGATTAGATACCCCCGAGCCCACGAGACCTAGAGGAGCCTG210.16034206306787815No Hit
GGTACACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTCTA210.16034206306787815No Hit
GGTACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGGAATCGC200.15270672673131253No Hit
GGTACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGGAATCG200.15270672673131253No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAACAG200.15270672673131253No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG190.1450713903947469No Hit
GGTACACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTAGG190.1450713903947469No Hit
GGTACACTGGGATTAGATACCCCTGACGTCTAGAGGAGCCTGTTCTAGGA180.13743605405818127No Hit
GGTACACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTAGGAATC180.13743605405818127No Hit
GGTACACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTAGGAAT170.12980071772161564No Hit
GGTACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT170.12980071772161564No Hit
GGTACACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTAGGAAT170.12980071772161564No Hit
GGTACACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCTG170.12980071772161564No Hit
GGTACACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAGGA170.12980071772161564No Hit
GGTACACTGGGATTAGATACCCCCGACCAACCTAGAGGAGCCTGTTCTAG170.12980071772161564No Hit
GGTACACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT160.12216538138505001No Hit
GGTACACTGGGATTAGATACCCCACGGACCTAGAGGAGCCTGTTCTAGGA160.12216538138505001No Hit
GGTACACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAGG160.12216538138505001No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTCAACAG160.12216538138505001No Hit
GGTACACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAGGAAT160.12216538138505001No Hit
GGTACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATAA150.11453004504848438No Hit
GGTACACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTAGGA150.11453004504848438No Hit
GGTACACTGGGATTAGATACCCCACTAGACCTAGAGGAGCCTGTTCTAGG150.11453004504848438No Hit
GGTACACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGGAAT150.11453004504848438No Hit
GGTACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTAG150.11453004504848438No Hit
GGTACACTGGGATTAGATACCCCGTGTAGTCCCTAGAGGAGCCTGTTCTA150.11453004504848438No Hit
GGTACACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAGGAATC140.10689470871191875No Hit
GGTACACTGGGATTAGATACCCCGAGCCACGAGACCTAGAGGAGCCTGTT140.10689470871191875No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGAT12800.0294.946357
CACTGGG12800.0294.946355
GGGATAG108.3741103E-4294.946359
ACACTGG12950.0293.807564
ACTGGGA12850.0293.79876
GGGATTA12800.0293.794229
TGGGATT12750.0293.78978
GGTACAC13100.0292.694851
GTACACT13100.0291.56912
TACACTG13050.0291.556183
CCAGAAA150.00281917196.6309295
CACTGGA150.00281917196.63095
TGGGATA150.00281917196.63098
ACTGGAT150.00281917196.63096
ACTTGAC257.6202214E-659.17064190-194
AGACTTG150.00431238459.17064190-194
CTTGACC257.6202214E-659.17064190-194
GACCTCG257.6202214E-659.17064195-199
GCCACAG4100.058.98927785-89
CACTACG4100.058.98927775-79
Produced by FastQC (version 0.11.5)