FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-37-5-4-1-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-37-5-4-1-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13060
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG383829.387442572741197No Hit
GGTACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTATT10538.062787136294029No Hit
GGTACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATTCCTCTGTC7505.742725880551302No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG7045.390505359877489No Hit
GGTACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATTCCTCT5193.9739663093415007No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC3202.4502297090352223No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG2792.1362940275650844No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTATT1971.5084226646248085No Hit
GGTACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATTCCTC1961.5007656967840737No Hit
GGTACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATTCCTCTGT1931.4777947932618682No Hit
GGTACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTATTCCTCTG1911.4624808575803983No Hit
GGTACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT1871.4318529862174578No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAT1621.2404287901990811No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT1621.2404287901990811No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG1431.0949464012251149No Hit
GGTACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTATTCCTCT1321.010719754977029No Hit
GGTACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTATTCCTC1260.9647779479326187No Hit
GGTACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT1020.781010719754977No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG970.7427258805513016No Hit
GGTACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG940.7197549770290965No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTATT790.6049004594180705No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG720.5513016845329249No Hit
GGTACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGCC710.5436447166921898No Hit
GGTACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTATTC620.4747320061255743No Hit
GGTACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTATTCCTC600.45941807044410415No Hit
GGTACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTATTCCTC600.45941807044410415No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG590.45176110260336905No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG560.4287901990811639No Hit
GGTACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG550.4211332312404288No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAT530.4058192955589586No Hit
GGTACACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTATTCCTCT480.3675344563552833No Hit
GGTACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG450.3445635528330781No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTATT430.329249617151608No Hit
GGTACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTATTCCTCT420.3215926493108729No Hit
GGTACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTATTCCTCT410.31393568147013784No Hit
GGTACACTGGGATTAGATACCCCGGGTAGTCTAGAGGAGCCTGTTCTATT400.3062787136294028No Hit
GGTACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTATTCC390.2986217457886677No Hit
GGTACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTATTCCTCT370.2833078101071976No Hit
GGTACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAT370.2833078101071976No Hit
GGTACACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTATT360.27565084226646247No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG350.2679938744257274No Hit
GGTACACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTATT340.26033690658499237No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG340.26033690658499237No Hit
GGTACACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTAG300.22970903522205208No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG300.22970903522205208No Hit
GGTACACTGGGATTAGATACCCCACTGACACTAGAGGAGCCTGTTCTATT300.22970903522205208No Hit
GGTACACTGGGATTAGATACCCCCGAGCCCACGAGACCTAGAGGAGCCTG290.222052067381317No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAT290.222052067381317No Hit
GGTACACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTATT290.222052067381317No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACTAGAGGAGCCTGTT280.21439509954058195No Hit
GGTACACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTATTCCT250.19142419601837674No Hit
GGTACACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTATTCCT250.19142419601837674No Hit
GGTACACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTATTCCTCTG240.18376722817764166No Hit
GGTACACTGGGATTAGATACCCCGGTAGCTAGAGGAGCCTGTTCTATTCC230.1761102603369066No Hit
GGTACACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTATTCCT230.1761102603369066No Hit
GGTACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTATTC230.1761102603369066No Hit
GGTACACTGGGATTAGATACCCCGACGACTAGAGGAGCCTGTTCTATTCC230.1761102603369066No Hit
GGTACACTGGGATTAGATACCCCGATCTAGAGGAGCCTGTTCTATTCCTC220.16845329249617153No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTCG220.16845329249617153No Hit
GGTACACTGGGATTAGATACCCCACTAGACCTAGAGGAGCCTGTTCTATT220.16845329249617153No Hit
GGTACACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTATTC210.16079632465543645No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG210.16079632465543645No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTATTC200.1531393568147014No Hit
GGTACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTATTCCTCTG190.14548238897396631No Hit
GGTACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTCT180.13782542113323124No Hit
GGTACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT170.13016845329249618No Hit
GGTACACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTATTCCTC170.13016845329249618No Hit
GGTACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT170.13016845329249618No Hit
GGTACACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGTTC170.13016845329249618No Hit
GGTACACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTATTCCT160.1225114854517611No Hit
GGTACACTGGGATTAGATACCCCTGACGCTGGACTAGAGGAGCCTGTTCT160.1225114854517611No Hit
GGTACACTGGGATTAGATACCCCGAGATGCCTAAGCTAGAGGAGCCTGTT160.1225114854517611No Hit
GGTACACTGGGATTAGATACCCCCCACCTAGAGGAGCCTGTTCTATTCCT160.1225114854517611No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTCAACAG160.1225114854517611No Hit
GGTACACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTATTCCTCT160.1225114854517611No Hit
GGTACACTGGGATTAGATACCCCCCGTCTAGAGGAGCCTGTTCTATTCCT150.11485451761102604No Hit
GGTACACTGGGATTAGATACCCCAATGTCAGACTAGAGGAGCCTGTTCTA150.11485451761102604No Hit
GGTACACTGGGATTAGATACCCCCGCCTAGAGGAGCCTGTTCTATTCCTC150.11485451761102604No Hit
GGTACACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTATTCC150.11485451761102604No Hit
GGTACACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTATT150.11485451761102604No Hit
GGTACACTGGGATTAGATACCCCGTCAGACTAGAGGAGCCTGTTCTATTC150.11485451761102604No Hit
GGTACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT140.10719754977029097No Hit
GGTACACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAGGT140.10719754977029097No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAG108.369551E-4295.09
CTGGGTT108.369551E-4295.07
CTGGGAT12600.0295.07
ACACTGG12900.0295.04
CACTGGG12700.0295.05
CACTGGA205.9550075E-8295.05
CTGGATT157.0778005E-6295.07
TGGATTA157.0778005E-6295.08
TGGGTTA108.369551E-4295.08
TGGGATT12550.0295.08
ACTGGGT108.369551E-4295.06
ACTGGGA12600.0295.06
ACTGGAT157.0778005E-6295.06
GGTACAC13000.0295.01
GGGATTA12600.0293.829389
TACACTG13050.0292.739473
GTACACT13100.0291.622132
CAGCCCT150.0043620459.00000430-34
AAACTTC303.2936077E-759.00000435-39
AAACTGC3600.059.00000460-64
Produced by FastQC (version 0.11.5)