FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-36-4-4-1-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-36-4-4-1-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13022
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG230717.71617263093227No Hit
GGTACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAGA145311.158040239594532No Hit
GGTACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGACGTGGCTG11899.130701889110735No Hit
GGTACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGACGTGG5093.908769774228229No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG3712.8490247273844265No Hit
GGTACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGACGTGGC3682.825986791583474No Hit
GGTACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGACGTGG3642.795269543848871No Hit
GGTACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGACGTG3332.557210873905698No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAGA3092.3729073874980804No Hit
GGTACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGACGTGGCT2892.219321148825065No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAGA2411.8507141760098293No Hit
GGTACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGACGTG2211.6971279373368149No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC1621.2440485332514206No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG1501.1518967900476118No Hit
GGTACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT1391.0674243587774535No Hit
GGTACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT1240.9522346797726924No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAGA1210.9291967439717401No Hit
GGTACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGACG920.7064966978958686No Hit
GGTACACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAGACGT900.6911380740285671No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG900.6911380740285671No Hit
GGTACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGACGTG880.6757794501612655No Hit
GGTACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGACGTGG840.6450622024266626No Hit
GGTACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTCT840.6450622024266626No Hit
GGTACACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAGA800.6143449546920596No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAG780.598986330824758No Hit
GGTACACTGGGATTAGATACCCCACTAGACCTAGAGGAGCCTGTTCTAGA690.5298725234219014No Hit
GGTACACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAGA660.5068345876209491No Hit
GGTACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAGAC610.46843802795269546No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG580.44540009215174325No Hit
GGTACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGACGTGG580.44540009215174325No Hit
GGTACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAG560.43004146828444173No Hit
GGTACACTGGGATTAGATACCCCTAGGATTGTTGGCAAGCTAGAGGAGCC540.4146828444171402No Hit
GGTACACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGACGTGG530.40700353248348947No Hit
GGTACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGACGTG530.40700353248348947No Hit
GGTACACTGGGATTAGATACCCCACGATACCTAGAGGAGCCTGTTCTAGA500.3839655966825372No Hit
GGTACACTGGGATTAGATACCCCTAGTTGACTAGAGGAGCCTGTTCTAGA500.3839655966825372No Hit
GGTACACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTAGACGT490.37628628474888653No Hit
GGTACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGAC480.36860697281523574No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG480.36860697281523574No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG440.33788972508063275No Hit
GGTACACTGGGATTAGATACCCCACGAGACATAGAGGAGCCTGTTCTAGA430.330210413146982No Hit
GGTACACTGGGATTAGATACCCCGTGTAGTCCCTAGAGGAGCCTGTTCTA430.330210413146982No Hit
GGTACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG420.3225311012133313No Hit
GGTACACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTAGACG410.3148517892796805No Hit
GGTACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCTA410.3148517892796805No Hit
GGTACACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAGAC400.3071724773460298No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG380.29181385347872835No Hit
GGTACACTGGGATTAGATACCCCCGAGCTAGAGGAGCCTGTTCTAGACGT350.2687759176777761No Hit
GGTACACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTAGA330.25341729381047456No Hit
GGTACACTGGGATTAGATACCCCTGAGCCCACGAGACCTAGAGGAGCCTG320.24573798187682386No Hit
GGTACACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCTG310.2380586699431731No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG310.2380586699431731No Hit
GGTACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG300.23037935800952233No Hit
GGTACACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAGACGT300.23037935800952233No Hit
GGTACACTGGGATTAGATACCCCTGACGTGCGGACTAGAGGAGCCTGTTC290.22270004607587163No Hit
GGTACACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAGACGT270.2073414222085701No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG270.2073414222085701No Hit
GGTACACTGGGATTAGATACCCCGACAACCTAGAGGAGCCTGTTCTAGAC270.2073414222085701No Hit
GGTACACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTAGACGT270.2073414222085701No Hit
GGTACACTGGGATTAGATACCCCCCACGAGACCTAGAGGAGCCTGTTCTA250.1919827983412686No Hit
GGTACACTGGGATTAGATACCCCTCTATACACATTACGCTAGAGGAGCCT250.1919827983412686No Hit
GGTACACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTAGACGTGGC250.1919827983412686No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT240.18430348640761787No Hit
GGTACACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAGACG240.18430348640761787No Hit
GGTACACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAGGT240.18430348640761787No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG230.17662417447396714No Hit
GGTACACTGGGATTAGATACCCCTGAGCCAACGAGACCTAGAGGAGCCTG230.17662417447396714No Hit
GGTACACTGGGATTAGATACCCCCGTGGACTAGAGGAGCCTGTTCTAGAC220.16894486254031638No Hit
GGTACACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAGACGT220.16894486254031638No Hit
GGTACACTGGGATTAGATACCCCCCACCTAGAGGAGCCTGTTCTAGACGT210.16126555060666564No Hit
GGTACACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAGACGTG210.16126555060666564No Hit
GGTACACTGGGATTAGATACCCCCGCCACCTAGAGGAGCCTGTTCTAGAC210.16126555060666564No Hit
GGTACACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAGACGT210.16126555060666564No Hit
GGTACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG210.16126555060666564No Hit
GGTACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGACGTGG210.16126555060666564No Hit
GGTACACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTTC200.1535862386730149No Hit
GGTACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAGAC190.14590692673936417No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAG190.14590692673936417No Hit
GGTACACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATATC190.14590692673936417No Hit
GGTACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGACGTGG190.14590692673936417No Hit
GGTACACTGGGATTAGATACCCCAAGCCCACGAGACCTAGAGGAGCCTGT190.14590692673936417No Hit
GGTACACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTAGAC180.1382276148057134No Hit
GGTACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT170.13054830287206268No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTCG170.13054830287206268No Hit
GGTACACTGGGATTAGATACCCCCGGACCTAGAGGAGCCTGTTCTAGACG160.12286899093841193No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG160.12286899093841193No Hit
GGTACACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAGACGTGG150.11518967900476117No Hit
GGTACACTGGGATTAGATACCCCGGCAGACCTAGAGGAGCCTGTTCTAGA150.11518967900476117No Hit
GGTACACTGGGATTAGATACCCCAGCTAGAGGAGCCTGTTCTAGACGTGG140.10751036707111043No Hit
GGTACACTGGGATTAGATACCCCACTGACACTAGAGGAGCCTGTTCTAGA140.10751036707111043No Hit
GGTACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT140.10751036707111043No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTGGG12650.0294.900025
ACACTGG12750.0294.94
CACTGGA108.3776825E-4294.95
TACACTG12750.0294.93
TACACGG108.3776825E-4294.93
CTGGATT108.3776825E-4294.97
CGGGATT108.3776825E-4294.97
TGGATTA108.3776825E-4294.98
ACGGGAT108.3776825E-4294.96
GGGATTG108.3776825E-4294.99
ACTGGAT108.3776825E-4294.96
CACGGGA108.3776825E-4294.95
ACTGGGA12700.0293.738986
GTACACT12850.0292.605072
CTGGGAT12750.0292.587077
TGGGATT12700.0292.577978
GGTACAC13050.0290.380461
GGGATTA12700.0290.255929
ACACGGG150.0028203717196.64
GTACACG150.0028203717196.62
Produced by FastQC (version 0.11.5)