FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-35-5-4-1-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-35-5-4-1-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12745
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG371929.180070615927818No Hit
GGTACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAGA10047.877599058454296No Hit
GGTACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGACGTGGCTG7996.269125147116517No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG6324.958807375441349No Hit
GGTACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGACGTGG3182.49509611612397No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG3032.377402903099255No Hit
GGTACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGACGTGG2832.2204786190663004No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC2752.157708905453119No Hit
GGTACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGACGTGGC2521.9772459788152217No Hit
GGTACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGACGTG2511.969399764613574No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAGA2071.624166339741075No Hit
GGTACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGACGTGGCT2061.6163201255394273No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAGA1671.3103177716751668No Hit
GGTACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT1581.2397018438603373No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG1260.9886229894076108No Hit
GGTACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGACGTG1210.9493919183993723No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT1160.9101608473911338No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAGA1120.8787759905845429No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG990.7767752059631228No Hit
GGTACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT910.7140054923499412No Hit
GGTACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG780.6120047077285209No Hit
GGTACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGACGTG710.557081208316987No Hit
GGTACACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAGA600.4707728520988623No Hit
GGTACACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAGACGT580.4550804236955669No Hit
GGTACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGACG560.43938799529227146No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAG550.43154178109062374No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG550.43154178109062374No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG540.4236955668889761No Hit
GGTACACTGGGATTAGATACCCCACTAGACCTAGAGGAGCCTGTTCTAGA500.3923107100823852No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG490.38446449588073756No Hit
GGTACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGACGTGG490.38446449588073756No Hit
GGTACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGACGTGG480.37661828167908984No Hit
GGTACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTCT460.3609258532757944No Hit
GGTACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG460.3609258532757944No Hit
GGTACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGACGTG460.3609258532757944No Hit
GGTACACTGGGATTAGATACCCCCGAGCCCACGAGACCTAGAGGAGCCTG410.32169478226755593No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG410.32169478226755593No Hit
GGTACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAGAC370.29030992546096507No Hit
GGTACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG370.29030992546096507No Hit
GGTACACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAGA360.2824637112593174No Hit
GGTACACTGGGATTAGATACCCCACGAGACATAGAGGAGCCTGTTCTAGA360.2824637112593174No Hit
GGTACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAG340.266771282856022No Hit
GGTACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGCC340.266771282856022No Hit
GGTACACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAGAC330.25892506865437426No Hit
GGTACACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGACGTGG320.25107885445272654No Hit
GGTACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGAC310.24323264025107885No Hit
GGTACACTGGGATTAGATACCCCCGAGCTAGAGGAGCCTGTTCTAGACGT300.23538642604943116No Hit
GGTACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCTA290.22754021184778345No Hit
GGTACACTGGGATTAGATACCCCTAGGATTGTTGGCAAGCTAGAGGAGCC280.21969399764613573No Hit
GGTACACTGGGATTAGATACCCCACGATACCTAGAGGAGCCTGTTCTAGA280.21969399764613573No Hit
GGTACACTGGGATTAGATACCCCTGAGCCCACGAGACCTAGAGGAGCCTG260.20400156924284032No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG250.1961553550411926No Hit
GGTACACTGGGATTAGATACCCCTAGTTGACTAGAGGAGCCTGTTCTAGA250.1961553550411926No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG240.18830914083954492No Hit
GGTACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT240.18830914083954492No Hit
GGTACACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAGACGT230.1804629266378972No Hit
GGTACACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTAGACGTGGC230.1804629266378972No Hit
GGTACACTGGGATTAGATACCCCTGAGCCAACGAGACCTAGAGGAGCCTG220.17261671243624951No Hit
GGTACACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTAGACG210.1647704982346018No Hit
GGTACACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTAGA210.1647704982346018No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAG210.1647704982346018No Hit
GGTACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT200.1569242840329541No Hit
GGTACACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTAGACGT200.1569242840329541No Hit
GGTACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATAA200.1569242840329541No Hit
GGTACACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAGACGT190.1490780698313064No Hit
GGTACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGACGTGG190.1490780698313064No Hit
GGTACACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAGACGTG180.1412318556296587No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTCAACAG180.1412318556296587No Hit
GGTACACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTAGAC170.133385641428011No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTCG170.133385641428011No Hit
GGTACACTGGGATTAGATACCCCGGCAGACCTAGAGGAGCCTGTTCTAGA170.133385641428011No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG160.12553942722636327No Hit
GGTACACTGGGATTAGATACCCCCGCCACCTAGAGGAGCCTGTTCTAGAC160.12553942722636327No Hit
GGTACACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAGACG160.12553942722636327No Hit
GGTACACTGGGATTAGATACCCCCCACGAGACCTAGAGGAGCCTGTTCTA150.11769321302471558No Hit
GGTACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT150.11769321302471558No Hit
GGTACACTGGGATTAGATACCCCCCACCTAGAGGAGCCTGTTCTAGACGT150.11769321302471558No Hit
GGTACACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTTC150.11769321302471558No Hit
GGTACACTGGGATTAGATACCCCTCTATACACATTACGCTAGAGGAGCCT150.11769321302471558No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG150.11769321302471558No Hit
GGTACACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTAGACGT140.10984699882306787No Hit
GGTACACTGGGATTAGATACCCCGTGTAGTCCCTAGAGGAGCCTGTTCTA140.10984699882306787No Hit
GGTACACTGGGATTAGATACCCCAGGGAGATCGAGGGTCTAGAGGAGCCT130.10200078462142016No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCGTAAACCTTGATAG130.10200078462142016No Hit
GGTACACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAGGT130.10200078462142016No Hit
GGTACACTGGGATTAGATACCCCTGACGTGCGGACTAGAGGAGCCTGTTC130.10200078462142016No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGAT12500.0295.07
GTACACT12650.0295.02
CACTGGG12550.0295.05
ACTGGGA12500.0295.06
GGTACAC12700.0293.83861
TGGGATT12500.0293.828
GGGATTA12450.0293.815259
TACACTG12750.0292.686283
ACACTGG12750.0291.529424
TGCCTCA1350.059.000004115-119
CCCGAGC452.5465852E-1159.00000420-24
ACCCCAC8050.059.00000415-19
AAGCGCC1050.059.00000480-84
TACCCCG1650.059.00000415-19
GGGAACT1050.059.00000470-74
AGACCCA1350.059.000004120-124
GACCTGG3600.059.000004105-109
GGCGGTG5400.059.000004110-114
AACGCTT257.749679E-659.090-94
AAACCTT800.059.035-39
Produced by FastQC (version 0.11.5)