FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-34-4-5-7-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-34-4-5-7-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12968
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC244518.854102405922273No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA12669.762492288710673No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACAGATCTG9087.001850709438619No Hit
CTGGATGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACAGAT5434.187230104873535No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT3892.999691548426897No Hit
CTGGATGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACAGA3812.9380012338062924No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACAGATC3802.9302899444787167No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA3142.421344848858729No Hit
CTGGATGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACAGA2682.0666255397902527No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA2431.8738433066008637No Hit
CTGGATGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC2381.8352868599629857No Hit
CTGGATGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACAGAT2351.8121529919802593No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT2051.5808143121529918No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT1801.3880320789636027No Hit
CTGGATGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAC1581.2183837137569402No Hit
CTGGATGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACAGATCT1531.1798272671190622No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT1331.025601480567551No Hit
CTGGATGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTACAGA1280.987045033929673No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA1240.9561998766193708No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT1080.8328192473781616No Hit
CTGGATGACTGGGATTAGATACCCCCCTAACACTAGAGGAGCCTGTTCTA920.7094386181369525No Hit
CTGGATGACTGGGATTAGATACCCCACTAACACTAGAGGAGCCTGTTCTA880.6785934608266502No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC860.663170882171499No Hit
CTGGATGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTACA800.6169031462060457No Hit
CTGGATGACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTT730.5629241209130167No Hit
CTGGATGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTACAGAT720.5552128315854411No Hit
CTGGATGACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAC710.5475015422578655No Hit
CTGGATGACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCC690.5320789636027143No Hit
CTGGATGACTGGGATTAGATACCCCAAGCTAGAGGAGCCTGTTCTACAGA650.5012338062924121No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT640.4935225169648365No Hit
CTGGATGACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAC570.4395434916718075No Hit
CTGGATGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT570.4395434916718075No Hit
CTGGATGACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAC520.4009870450339297No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGCTAGAGGAGCCTGTTCTACA510.39327575570635415No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC510.39327575570635415No Hit
CTGGATGACTGGGATTAGATACCCCTGACCCTAGAGGAGCCTGTTCTACA510.39327575570635415No Hit
CTGGATGACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTACAGAT500.3855644663787785No Hit
CTGGATGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT500.3855644663787785No Hit
CTGGATGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAC480.3701418877236274No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCACTAGAGGAGCCTGTTCTAC480.3701418877236274No Hit
CTGGATGACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTACAG460.35471930906847626No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT450.3470080197409007No Hit
CTGGATGACTGGGATTAGATACCCCCCACGAGACCTAGAGGAGCCTGTTC450.3470080197409007No Hit
CTGGATGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTACAGAT450.3470080197409007No Hit
CTGGATGACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACT430.3315854410857495No Hit
CTGGATGACTGGGATTAGATACCCCTGACGTGCTAGAGGAGCCTGTTCTA430.3315854410857495No Hit
CTGGATGACTGGGATTAGATACCCCTGACCGACGACTAGAGGAGCCTGTT420.32387415175817397No Hit
CTGGATGACTGGGATTAGATACCCCGACGACTAGAGGAGCCTGTTCTACA410.31616286243059843No Hit
CTGGATGACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTACAG410.31616286243059843No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC410.31616286243059843No Hit
CTGGATGACTGGGATTAGATACCCCAATATGCTAGAGGAGCCTGTTCTAC380.2930289944478717No Hit
CTGGATGACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTA380.2930289944478717No Hit
CTGGATGACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGT370.28531770512029614No Hit
CTGGATGACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTACAGA340.26218383713756943No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT330.25447254780999384No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT330.25447254780999384No Hit
CTGGATGACTGGGATTAGATACCCCTCTATACACATTACGCTAGAGGAGC320.24676125848241826No Hit
CTGGATGACTGGGATTAGATACCCCAACGAGACCTAGAGGAGCCTGTTCT320.24676125848241826No Hit
CTGGATGACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATA300.23133867982726714No Hit
CTGGATGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTACAGAT300.23133867982726714No Hit
CTGGATGACTGGGATTAGATACCCCCGCAACCTAGAGGAGCCTGTTCTAC280.215916101172116No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT270.2082048118445404No Hit
CTGGATGACTGGGATTAGATACCCCAGTGCAGCTGTTGGAGACTAGAGGA250.19278223318938925No Hit
CTGGATGACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTACAGAT240.1850709438618137No Hit
CTGGATGACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGT240.1850709438618137No Hit
CTGGATGACTGGGATTAGATACCCCGAGCAACCTAGAGGAGCCTGTTCTA240.1850709438618137No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT230.17735965453423813No Hit
CTGGATGACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTACAG230.17735965453423813No Hit
CTGGATGACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTACAG230.17735965453423813No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT210.16193707587908698No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT210.16193707587908698No Hit
CTGGATGACTGGGATTAGATACCCCCAGACTAGAGGAGCCTGTTCTACAG210.16193707587908698No Hit
CTGGATGACTGGGATTAGATACCCCTATAAGAGGAGCCTGTTCTACAGAT200.15422578655151142No Hit
CTGGATGACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCT200.15422578655151142No Hit
CTGGATGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA190.14651449722393586No Hit
CTGGATGACTGGGATTAGATACCCTATAGAGGAGCCTGTTCTACAGATCT190.14651449722393586No Hit
CTGGATGACTGGGATTAGATACCCCGTCAGACTAGAGGAGCCTGTTCTAC190.14651449722393586No Hit
CTGGATGACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTACA180.13880320789636028No Hit
CTGGATGACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTACAGATC180.13880320789636028No Hit
CTGGATGACTGGGATTAGATACCCCTATAAAGGGCTAGAGGAGCCTGTTC180.13880320789636028No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT180.13880320789636028No Hit
CTGGATGACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTACAG170.13109191856878472No Hit
CTGGATGACTGGGATTAGATACCCCCGAGAAGACCTAGAGGAGCCTGTTC160.12338062924120913No Hit
CTGGATGACTGGGATTAGATACCCCCACGACCTAGAGGAGCCTGTTCTAC160.12338062924120913No Hit
CTGGATGACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTACAG160.12338062924120913No Hit
CTGGATGACTGGGATTAGATACCCCCACGAACCTAGAGGAGCCTGTTCTA160.12338062924120913No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCATAGAGGAGCCTGTTCTA150.11566933991363357No Hit
CTGGATGACTGGGATTAGATACCCCGATCTAGAGGAGCCTGTTCTACAGA150.11566933991363357No Hit
CTGGATGACTGGGATTAGATACCCCAAACCTAGAGGAGCCTGTTCTACAG140.107958050586058No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGCTAGAGGAGCCTGTTCTACA140.107958050586058No Hit
CTGGATGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT130.10024676125848242No Hit
CTGGATGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG130.10024676125848242No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGAC12900.0295.03
CTGGGAT12650.0295.09
GATGACT12900.0295.04
CTGGATG12950.0295.01
TGGATGA12950.0295.02
TGACTGG12950.0293.8616
GACTGGG12800.0293.847637
ACTGGGA12700.0293.83868
ATGACTG13050.0291.60925
CTGGATT202.230575E-5221.249989
GACTGGA202.230575E-5221.249987
GAGCGAA150.0028173868196.66666295
ACTGGAT255.4312244E-5177.08
CGCCAGG900.059.00000470-74
CGCCAGC1000.059.00000485-89
CCGTCTA501.8189894E-1259.00000420-24
AACCTTG501.8189894E-1259.00000440-44
TGCCTCA900.059.000004115-119
CCCGTCT501.8189894E-1259.00000420-24
CCCCGTC501.8189894E-1259.00000420-24
Produced by FastQC (version 0.11.5)