FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-33-5-4-1-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-33-5-4-1-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12681
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG421333.222931945430176No Hit
GGTACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAAC10127.980443182714297No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG6385.031148963015535No Hit
GGTACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAACTTCGCCTG6034.755145493257629No Hit
GGTACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAACTTCGC3853.0360381673369607No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC2992.3578582130746786No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG2842.239571011749862No Hit
GGTACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT2411.9004810346187209No Hit
GGTACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAACTTCG2321.8295087138238308No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAAC1911.5061903635359986No Hit
GGTACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAACTTCGCC1681.3248166548379465No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG1561.230186893778093No Hit
GGTACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAACTTCGCCT1281.0093841179717689No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT1230.9699550508634965No Hit
GGTACACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAACTTC1210.9541834240201876No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAA1070.8437820361170255No Hit
GGTACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT1010.7964671555870988No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG920.7254948347922089No Hit
GGTACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAA850.6702941408406277No Hit
GGTACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAACT850.6702941408406277No Hit
GGTACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG810.63875088715401No Hit
GGTACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAACTTCGC770.6072076334673921No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAAC770.6072076334673921No Hit
GGTACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAACTTCG670.5283494992508477No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG650.5125778724075388No Hit
GGTACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAACTTCG600.4731488052992667No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG590.4652629918776122No Hit
GGTACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAACT590.4652629918776122No Hit
GGTACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG570.44949136503430326No Hit
GGTACACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAACTTCGC550.4337197381909944No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG550.4337197381909944No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG440.3469757905527955No Hit
GGTACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCTA440.3469757905527955No Hit
GGTACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAACTT410.3233183502878322No Hit
GGTACACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTAACT410.3233183502878322No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG400.3154325368661778No Hit
GGTACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG400.3154325368661778No Hit
GGTACACTGGGATTAGATACCCCACGATACCTAGAGGAGCCTGTTCTAAC370.2917750966012144No Hit
GGTACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT340.26811765633625106No Hit
GGTACACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTAA340.26811765633625106No Hit
GGTACACTGGGATTAGATACCCCTGACCTGCTAGAGGAGCCTGTTCTAAC330.2602318429145966No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAAC330.2602318429145966No Hit
GGTACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAACTTCGC320.2523460294929422No Hit
GGTACACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAACTTC310.24446021607128776No Hit
GGTACACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAACTTCGC310.24446021607128776No Hit
GGTACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAACT290.22868858922797886No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG280.2208027758063244No Hit
GGTACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAACTTCG270.21291696238466998No Hit
GGTACACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAACTT260.20503114896301555No Hit
GGTACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGCC260.20503114896301555No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAA230.18137370869805222No Hit
GGTACACTGGGATTAGATACCCCAAACTAGAGGAGCCTGTTCTAACTTCG210.16560208185474332No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG210.16560208185474332No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAA210.16560208185474332No Hit
GGTACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT200.1577162684330889No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACTAGAGGAGCCTGTT180.14194464158977999No Hit
GGTACACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAACT180.14194464158977999No Hit
GGTACACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAACTTCGC180.14194464158977999No Hit
GGTACACTGGGATTAGATACCCCTATAAAGGGCTAGAGGAGCCTGTTCTA180.14194464158977999No Hit
GGTACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT170.13405882816812553No Hit
GGTACACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTAG170.13405882816812553No Hit
GGTACACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTAACTTC170.13405882816812553No Hit
GGTACACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAACTTC170.13405882816812553No Hit
GGTACACTGGGATTAGATACCCCCGTCCTAGAGGAGCCTGTTCTAACTTC160.1261730147464711No Hit
GGTACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAACTTCGC160.1261730147464711No Hit
GGTACACTGGGATTAGATACCCCGACAATCTAGAGGAGCCTGTTCTAACT160.1261730147464711No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTCG150.11828720132481667No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAACAG150.11828720132481667No Hit
GGTACACTGGGATTAGATACCCCTGACGTGCGGACTAGAGGAGCCTGTTC150.11828720132481667No Hit
GGTACACTGGGATTAGATACCCCGTGTAGTCCCTAGAGGAGCCTGTTCTA150.11828720132481667No Hit
GGTACACTGGGATTAGATACCCCCGACGCTAGAGGAGCCTGTTCTAACTT150.11828720132481667No Hit
GGTACACTGGGATTAGATACCCCCGCAACCTAGAGGAGCCTGTTCTAACT140.1104013879031622No Hit
GGTACACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAAC140.1104013879031622No Hit
GGTACACTGGGATTAGATACCCCCCGACCTAGAGGAGCCTGTTCTAACTT140.1104013879031622No Hit
GGTACACTGGGATTAGATACCCCACGAACCTAGAGGAGCCTGTTCTAACT130.10251557448150778No Hit
GGTACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATAA130.10251557448150778No Hit
GGTACACTGGGATTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT130.10251557448150778No Hit
GGTACATGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT130.10251557448150778No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATAG108.3665055E-4295.09
TGGGATA108.3665055E-4295.08
ACACTGG12450.0293.815254
CACTGGG12250.0293.795935
GGTACAC12600.0292.658721
CTGGGAT12250.0292.591837
GGGATTA12150.0292.572029
TGGGATT12150.0292.572028
ACTGGGA12300.0291.402446
TACACTG12700.0289.192933
GTACACT12800.0288.085942
CACTGGA202.2293472E-5221.255
CTGGATT150.0028166138196.666667
TGGATTA150.0028166138196.666668
ACTGGAT255.4282413E-5177.06
AACGCTT257.749322E-659.00000490-94
CCAACTA257.749322E-659.00000420-24
AATCTTG257.749322E-659.00000440-44
AACCGCT501.8189894E-1259.00000460-64
GCCTCAG1650.059.000004115-119
Produced by FastQC (version 0.11.5)