FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-32-6-4-6-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-32-6-4-6-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12576
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA598947.62245547073791No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA6415.097010178117048No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA4243.371501272264631No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA3172.520674300254453No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATTCCTCTGT2471.964058524173028No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA2241.7811704834605597No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT2061.6380407124681935No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA2051.6300890585241732No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAT1961.55852417302799No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA1841.4631043256997456No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATA1611.2802162849872774No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA1381.0973282442748091No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA1110.8826335877862597No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATTCCTC1050.8349236641221374No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA1030.8190203562340967No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA950.7554071246819338No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA900.7156488549618321No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA890.7076972010178118No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATTCCTCTG600.4770992366412214No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATA550.4373409669211196No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACATTGGTA520.4134860050890585No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA510.40553435114503816No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTATTCCTCT470.37372773536895676No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCATAAACATTGACA470.37372773536895676No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT440.34987277353689566No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATC420.33396946564885494No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATCAACAAAACCATT410.3260178117048346No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG400.3180661577608142No Hit
GCCACAACTGGGATTAGATACCCCACTACGCTTAGCCCTAAAGTCGAATA390.3101145038167939No Hit
GCCACAACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCCAATA390.3101145038167939No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTCCTAAACCTCAACA380.30216284987277353No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATAGATA380.30216284987277353No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAGATA370.29421119592875317No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT370.29421119592875317No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCCAAACCTCAACA360.2862595419847328No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAGATA360.2862595419847328No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAACA360.2862595419847328No Hit
GCCACAACTGGGATTAGATACCCCACCAGGCTTAGCCCTAAACTCCAATA350.2783078880407125No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACA340.27035623409669207No Hit
GCCACAACTGGGATTAGATACCCCCTATGCTTAGCCCTAAACTCTAATAG340.27035623409669207No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAACA330.26240458015267176No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG330.26240458015267176No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATTCCT330.26240458015267176No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA330.26240458015267176No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAACA310.24650127226463103No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAGAACCATT300.2385496183206107No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAT300.2385496183206107No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCTCTAAAGTCGAATA300.2385496183206107No Hit
GCCACAACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATA300.2385496183206107No Hit
GCCACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG280.22264631043256997No Hit
GCCACAACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTATTCC280.22264631043256997No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACA270.21469465648854963No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCATAAACTTTGATA250.1987913486005089No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC250.1987913486005089No Hit
GCCACAACTGGGATTAGATACCCCCATCTAGAGGAGCCTGTTCTATTCCT230.1828880407124682No Hit
GCCACAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTATTCCTC220.17493638676844783No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGATG220.17493638676844783No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCATAAACTTAAATA210.16698473282442747No Hit
GCCACAACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTATTCCTCT200.1590330788804071No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGTA200.1590330788804071No Hit
GCCACAACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAATA200.1590330788804071No Hit
GCCACAACTGGGATTAGATACCCCAGAAGGACATATGCCAATTGAAGTCT190.15108142493638677No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATCAACA180.1431297709923664No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACATGGATA180.1431297709923664No Hit
GCCACAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG170.13517811704834604No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCCAAATA170.13517811704834604No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTTATG170.13517811704834604No Hit
GCCACAACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTATT160.1272264631043257No Hit
GCCACAACTGGGATTAGATACCCCACCGACCAAAAAAGGTTTCCAATTAC160.1272264631043257No Hit
GCCACAACTGGGATTAGATACCCCTCGGAGATGTGTATAAGAGACAGAGG160.1272264631043257No Hit
GCCACAACTGGGATTAGATACCCCTATAAGAGACAGAGGAGCCTGTTCTA160.1272264631043257No Hit
GCCACAACTGGGATTAGATACCCCCGAGCCCACGAGACCTAGAGGAGCCT160.1272264631043257No Hit
GCCACAACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA150.11927480916030535No Hit
GCCACAACTGGGATTAGATACCCCTGATCAAGCTGCCTGGGCCCGAGAAC150.11927480916030535No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATA140.11132315521628498No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTAGATC140.11132315521628498No Hit
GCCACAACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTATTCCTCT140.11132315521628498No Hit
GCCACAACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTATTCCTC140.11132315521628498No Hit
GCCACAACTGGGATTGATACCCCACTATGCTTAGCCCTAAACCTCAACAG140.11132315521628498No Hit
GCCACAACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTATTCCTC140.11132315521628498No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGGG11950.0295.06
AACTGGA255.002221E-10295.06
CAACTGG12250.0295.05
CTGGATT205.9459126E-8295.08
TGGATTA205.9459126E-8295.09
GCCACAC108.3657133E-4295.01
ACTGGAT205.9459126E-8295.07
CTGGGAT11950.0293.765728
ACTGGGA11950.0293.765727
TGGGATT11850.0293.755289
GCCACAA12550.0292.64941
CACAACT12650.0288.003943
ACAACTG12650.0286.837924
CCACAAC13000.0282.519232
CACAACG200.006659047147.53
TATAAAA652.1028798E-590.76923295
CACATAA700.003256779463.214283295
TCCTCTA150.004361260759.000004125-129
AAACCCT303.2915523E-759.00000440-44
CCAGCAA150.004361260759.00000480-84
Produced by FastQC (version 0.11.5)