FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-31-5-4-1-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-31-5-4-1-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12663
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG375829.677011766564004No Hit
GGTACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTACC9437.446892521519387No Hit
GGTACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCTAGCTGTC6835.393666587696439No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG6395.046197583511016No Hit
GGTACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACCTAGCT4703.7116007265260995No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTACC3542.7955460791281688No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC2722.1479902076917003No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG2511.9821527284213851No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT2091.650477769880755No Hit
GGTACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT1891.4925373134328357No Hit
GGTACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACCTAGCT1871.4767432677880439No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTACC1811.4293611308536682No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG1771.3977730395640844No Hit
GGTACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACCTAGCTGT1551.2240385374713734No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAC1461.1529653320698097No Hit
GGTACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACCTAGC1361.0739951038458502No Hit
GGTACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACCTAGCTG1260.9950248756218906No Hit
GGTACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACCTAGC1200.9476427386875147No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTACC960.7581141909500119No Hit
GGTACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAC930.734423122482824No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG790.6238648029692806No Hit
GGTACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG770.6080707573244887No Hit
GGTACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTACCTA750.5922767116796968No Hit
GGTACACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTACC700.552791597567717No Hit
GGTACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTACCTAGCT660.5212035062781332No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG650.5133064834557372No Hit
GGTACACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTACCT570.4501303008765696No Hit
GGTACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTACCTAGC510.40274816394219376No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG450.35536602700781805No Hit
GGTACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTACCTAGCT440.3474690041854221No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG430.33957198136302613No Hit
GGTACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG410.3237779357182342No Hit
GGTACACTGGGATTAGATACCCCTGACCCTAGAGGAGCCTGTTCTACCTA390.3079838900734423No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG390.3079838900734423No Hit
GGTACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT380.30008686725104633No Hit
GGTACACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTACCTAGCT370.29218984442865037No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTACCT370.29218984442865037No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG350.2763957987838585No Hit
GGTACACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTACCTAG350.2763957987838585No Hit
GGTACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG350.2763957987838585No Hit
GGTACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTACCTAGC320.2527047303166706No Hit
GGTACACTGGGATTAGATACCCCTCGAGACCTAGAGGAGCCTGTTCTACC320.2527047303166706No Hit
GGTACACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTACCTAGCT320.2527047303166706No Hit
GGTACACTGGGATTAGATACCCCACGGACCTAGAGGAGCCTGTTCTACCT310.2448077074942747No Hit
GGTACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTACCT310.2448077074942747No Hit
GGTACACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTACCTAG310.2448077074942747No Hit
GGTACACTGGGATTAGATACCCCACTAACACTAGAGGAGCCTGTTCTACC300.23691068467187867No Hit
GGTACACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTAG300.23691068467187867No Hit
GGTACACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTACCTAG300.23691068467187867No Hit
GGTACACTGGGATTAGATACCCCTGAGCCCACGAGACCTAGAGGAGCCTG290.22901366184948274No Hit
GGTACACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTACC290.22901366184948274No Hit
GGTACACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTACCTAGCTG290.22901366184948274No Hit
GGTACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTACCT280.22111663902708678No Hit
GGTACACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTACCTA280.22111663902708678No Hit
GGTACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCTA280.22111663902708678No Hit
GGTACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTACCT270.21321961620469082No Hit
GGTACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTACCTAGCT260.2053225933822949No Hit
GGTACACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTACC250.19742557055989893No Hit
GGTACACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTAC250.19742557055989893No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG240.18952854773750297No Hit
GGTACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT240.18952854773750297No Hit
GGTACACTGGGATTAGATACCCCTCTTATCACGTCTGACGCTAGAGGAGC240.18952854773750297No Hit
GGTACACTGGGATTAGATACCCCCAAGACCTAGAGGAGCCTGTTCTACCT230.181631524915107No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAC220.17373450209271105No Hit
GGTACACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTACCT220.17373450209271105No Hit
GGTACACTGGGATTAGATACCCCCGTGGACTAGAGGAGCCTGTTCTACCT200.15794045644791915No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCTAGAGGAGCCTGTTCTACCT190.15004343362552316No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAACCTCAACAG180.14214641080312723No Hit
GGTACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTACCT180.14214641080312723No Hit
GGTACACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAGGT180.14214641080312723No Hit
GGTACACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTACCTAG170.13424938798073127No Hit
GGTACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT160.1263523651583353No Hit
GGTACACTGGGATTAGATACCCCTGAGAACTAGAGGAGCCTGTTCTACCT160.1263523651583353No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG150.11845534233593934No Hit
GGTACACTGGGATTAGATACCCCTATAAAGGGCTAGAGGAGCCTGTTCTA150.11845534233593934No Hit
GGTACACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTACCTAGCT140.11055831951354339No Hit
GGTACACTGGGATTAGATACCCCTGACTAGAGGAGCCTGTTCTACCTAGC140.11055831951354339No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAACAG140.11055831951354339No Hit
GGTACACTGGGATTAGATACCCCTGTATAAGAGGAGCCTGTTCTACCTAG140.11055831951354339No Hit
GGTACACTGGGATTAGATACCCCCACACCTAGAGGAGCCTGTTCTACCTA130.10266129669114744No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTCG130.10266129669114744No Hit
GGTACACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTACC130.10266129669114744No Hit
GGTACACTGGGATTAGATACCCCATGTCAGACTAGAGGAGCCTGTTCTAC130.10266129669114744No Hit
GGTACACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTACCTAG130.10266129669114744No Hit
GGTACACTGGGATTAGATACCCCTCTCCGAGCCCACGAGACCTAGAGGAG130.10266129669114744No Hit
GGTACACTGGGATTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT130.10266129669114744No Hit
GGTACACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCCTA130.10266129669114744No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTGGG12450.0294.86965
CTGGGGA108.377595E-4294.869577
CTGGGAT12250.0294.869577
ACACTGG12600.0294.869574
GTACACT12600.0294.869572
CACTGGA157.0851365E-6294.869575
TGGGGAT108.377595E-4294.869578
CTGGATT157.0851365E-6294.869577
TGGATTA157.0851365E-6294.869578
TGGGATT12250.0294.869578
ACTGGGG157.0851365E-6294.869576
ACTGGGA12250.0294.869576
GGTACAC12650.0294.869571
GGGATTA12200.0293.661079
TACACTG12700.0292.547763
ACTGGAT202.233284E-5221.152166
GGGATTG150.0028203435196.57979
GCCCTAA5150.059.2079430-34
AAACCTT1000.059.2079435-39
CGCCAGG1350.059.2079465-69
Produced by FastQC (version 0.11.5)