FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-30-4-2-6-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-30-4-2-6-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12959
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA240618.56624739563238No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAC172613.318928929701364No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACAGATCTGT11388.781541785631608No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA5464.21328806235049No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACAGATC4003.08665792113589No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA3182.453893047303033No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAC3142.4230264680916735No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACAGATCTG3042.3458600200632764No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT2461.8982946214985725No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA1991.5356123157651054No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC1971.520179026159426No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACAGAT1831.4121459989196699No Hit
GCCACAACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA1711.319546261285593No Hit
GCCACAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACAGATC1531.1806466548344778No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACAGATCT1491.1497800756231191No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAC1230.9491473107492863No Hit
GCCACAACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTACAGAT1090.84111428350953No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACAGAT1050.8102477042981713No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA1020.787097769889652No Hit
GCCACAACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAC970.7485145458754534No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA860.6636314530442164No Hit
GCCACAACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC820.6327648738328574No Hit
GCCACAACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTA820.6327648738328574No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA820.6327648738328574No Hit
GCCACAACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTACA800.617331584227178No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAC800.617331584227178No Hit
GCCACAACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTACAG800.617331584227178No Hit
GCCACAACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTACAGAT780.6018982946214986No Hit
GCCACAACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTACA710.5478817810016204No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA640.49386526738174247No Hit
GCCACAACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTACAGA630.48614862257890273No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA560.4321321089590246No Hit
GCCACAACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTACA540.41669881935334513No Hit
GCCACAACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTACA530.4089821745505055No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA530.4089821745505055No Hit
GCCACAACTGGGATTAGATACCCCTGACCTAGAGGAGCCTGTTCTACAGA470.3626823057334671No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA440.3395323713249479No Hit
GCCACAACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTACAGATC430.3318157265221082No Hit
GCCACAACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTACAG430.3318157265221082No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTACA420.3240990817192685No Hit
GCCACAACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTACAGA410.3163824369164287No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCTA390.3009491473107493No Hit
GCCACAACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTA380.29323250250790955No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA370.2855158577050698No Hit
GCCACAACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT370.2855158577050698No Hit
GCCACAACTGGGATTAGATACCCCGAGCCACGAGACTAGAGGAGCCTGTT360.2777992129022301No Hit
GCCACAACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTACAGATC350.27008256809939035No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA350.27008256809939035No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGTGCCTAGAGGAGCC330.25464927849371094No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA330.25464927849371094No Hit
GCCACAACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAC330.25464927849371094No Hit
GCCACAACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTACAGAT320.24693263369087123No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA310.2392159888880315No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT310.2392159888880315No Hit
GCCACAACTGGGATTAGATACCCCTGACGCTCTAGAGGAGCCTGTTCTAC300.23149934408519174No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA300.23149934408519174No Hit
GCCACAACTGGGATTAGATACCCCTACCCTAGAGGAGCCTGTTCTACAGA300.23149934408519174No Hit
GCCACAACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTACAGATC290.22378269928235206No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGTCTAGAGGAGCCTGTTCTAC290.22378269928235206No Hit
GCCACAACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTACAGA290.22378269928235206No Hit
GCCACAACTGGGATTAGATACCCCAATGAGATCGAGGGCCTAGAGGAGCC280.2160660544795123No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA280.2160660544795123No Hit
GCCACAACTGGGATTAGATACCCCAATGTCAGACTAGAGGAGCCTGTTCT270.20834940967667256No Hit
GCCACAACTGGGATTAGATACCCCACGAAACTAGAGGAGCCTGTTCTACA250.19291612007099312No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC250.19291612007099312No Hit
GCCACAACTGGGATTAGATACCCCCACAGAGAGCTAGAGGAGCCTGTTCT240.18519947526815342No Hit
GCCACAACTGGGATTAGATACCCCACCCGACCTAGAGGAGCCTGTTCTAC220.16976618566247395No Hit
GCCACAACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTACAGATC220.16976618566247395No Hit
GCCACAACTGGGATTAGATACCCCCGGACCTAGAGGAGCCTGTTCTACAG220.16976618566247395No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA220.16976618566247395No Hit
GCCACAACTGGGATTAGATACCCCCGAGATCTAGAGGAGCCTGTTCTACA210.16204954085963424No Hit
GCCACAACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCCT210.16204954085963424No Hit
GCCACAACTGGGATTAGATACCCCAGGACCTAGAGGAGCCTGTTCTACAG200.1543328960567945No Hit
GCCACAACTGGGATTAGATACCCCCCGCTAGAGGAGCCTGTTCTACAGAT200.1543328960567945No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG200.1543328960567945No Hit
GCCACAACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTACAGA190.14661625125395478No Hit
GCCACAACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTACAGA190.14661625125395478No Hit
GCCACAACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTACAGA190.14661625125395478No Hit
GCCACAACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTACAG180.13889960645111504No Hit
GCCACAACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATAT180.13889960645111504No Hit
GCCACAACTGGGATTAGATACCCCCACGAGATCTAGAGGAGCCTGTTCTA180.13889960645111504No Hit
GCCACAACTGGGATTAGATACCCCACGGGACCTAGAGGAGCCTGTTCTAC170.13118296164827534No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA160.12346631684543562No Hit
GCCACAACTGGGATTAGATACCCCTGTCAGACTAGAGGAGCCTGTTCTAC160.12346631684543562No Hit
GCCACAACTGGGATTAGATACCCCGACCAACCTAGAGGAGCCTGTTCTAC160.12346631684543562No Hit
GCCACAACTGGGATTAGATACCCCAAGATCTAGAGGAGCCTGTTCTACAG150.11574967204259587No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG150.11574967204259587No Hit
GCCACAACTGGGATTAGATACCCCGATCTAGAGGAGCCTGTTCTACAGAT140.10803302723975615No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA140.10803302723975615No Hit
GCCACAACTGGGATTAGATACCCCCACCCTAGAGGAGCCTGTTCTACAGA140.10803302723975615No Hit
GCCACAACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTA140.10803302723975615No Hit
GCCACAACTGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTACA130.10031638243691643No Hit
GCCACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG130.10031638243691643No Hit
GCCACAACTGGGATTAGATACCCCAGACGCTGCTAGAGGAGCCTGTTCTA130.10031638243691643No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGGG12550.0295.06
CTGGGAT12500.0295.08
CAACTGG12750.0295.05
TGGGATT12500.0295.09
ACTGGGA12500.0295.07
CTGGATT205.9531885E-8294.999978
TGGATTA205.9531885E-8294.999979
GCCACAA13000.0292.730771
CCACAAC13250.0287.207552
ACAACTG13500.0280.79634
CACAACT13700.0276.697083
AACTGGA251.8117498E-7236.06
ACTGGAT251.8117498E-7236.07
CACTGAA352.0753541E-4126.428566295
AATCTTG257.751396E-659.00000440-44
TAAACTT501.8189894E-1259.00000435-39
AACCGCT257.751396E-659.00000460-64
ATCTTGA257.751396E-659.00000440-44
AAATCTT257.751396E-659.00000440-44
CTTTACC257.751396E-659.00000455-59
Produced by FastQC (version 0.11.5)