FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-3-6-5-8-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-3-6-5-8-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10836
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA506146.70542635658915No Hit
TGATTGACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGCTGAGA5124.724990771502399No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGA3673.386858619416759No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA3032.796234772978959No Hit
TGATTGACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCA2572.37172388335179No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAA2492.2978959025470655No Hit
TGATTGACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAA1961.8087855297157622No Hit
TGATTGACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGCTGAG1841.6980435585086748No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGA1801.6611295681063125No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAA1691.5596160944998154No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAA1571.448874123292728No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGA1361.2550756736803248No Hit
TGATTGACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAA1221.1258767072720561No Hit
TGATTGACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAA1121.03359173126615No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAA1070.9874492432631967No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAA1050.9689922480620154No Hit
TGATTGACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCC850.784422296050203No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAA840.7751937984496124No Hit
TGATTGACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGCTGA680.6275378368401624No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGA660.6090808416389812No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGA620.5721668512366187No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCT560.516795865633075No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAA500.46142488002953125No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGA480.4429678848283499No Hit
TGATTGACACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTAGCTGA470.43373938722775934No Hit
TGATTGACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAA350.32299741602067183No Hit
TGATTGACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGCTG320.29531192321889993No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAGA280.2583979328165375No Hit
TGATTGACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAG250.23071244001476562No Hit
TGATTGACACTGGGATTAGATACCCCTCTCCTAGAGGAGCCTGTTCTAGC240.22148394241417496No Hit
TGATTGACACTGGGATTAGATACCCCTCCTAGAGGAGCCTGTTCTAGCTG240.22148394241417496No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCCACGAGACACCATCCAATC220.2030269472129937No Hit
TGATTGACACTGGGATTAGATACCCCACGATAGAGGAGCCTGTTCTAGCT220.2030269472129937No Hit
TGATTGACACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTAGCTG210.1937984496124031No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGA210.1937984496124031No Hit
TGATTGACACTGGGATTAGATACCCCAATATGCTAGAGGAGCCTGTTCTA210.1937984496124031No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAA200.18456995201181248No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAA200.18456995201181248No Hit
TGATTGACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAAC190.17534145441122187No Hit
TGATTGACACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTAGC190.17534145441122187No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAA190.17534145441122187No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAA180.16611295681063123No Hit
TGATTGACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGCTG180.16611295681063123No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTA180.16611295681063123No Hit
TGATTGACACTGGGATTAGATACCCCTCGTCTAGAGGAGCCTGTTCTAGC180.16611295681063123No Hit
TGATTGACACTGGGATTAGATACCCCACCATGCTTAGCCCTAAACCTCAA180.16611295681063123No Hit
TGATTGACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC180.16611295681063123No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTAG170.1568844592100406No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGA170.1568844592100406No Hit
TGATTGACACTGGGATTAGATACCCCGAGACTAGAGGAGCCTGTTCTAGC160.14765596160944996No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTAA160.14765596160944996No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAA160.14765596160944996No Hit
TGATTGACACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAA160.14765596160944996No Hit
TGATTGACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTC160.14765596160944996No Hit
TGATTGACACTGGGATTAGATACCCCTCCGAGCCCACGAGACACCATCCA150.13842746400885936No Hit
TGATTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC150.13842746400885936No Hit
TGATTGACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGCT150.13842746400885936No Hit
TGATTGACACTGGGATTAGATACCCCCTCCCCTAGAGGAGCCTGTTCTAG140.12919896640826875No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATCAA140.12919896640826875No Hit
TGATTGACACTGGGATTAGATACCCCTCCAGCTAGAGGAGCCTGTTCTAG130.11997046880767812No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAG130.11997046880767812No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCC130.11997046880767812No Hit
TGATTGACACTGGGATTGATACCCCACTATGCTTAGCCCTAAACCTCAAC120.11074197120708748No Hit
TGATTGACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTC110.10151347360649685No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAA110.10151347360649685No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCCAAACCTCAA110.10151347360649685No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAGCCTCAA110.10151347360649685No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAA110.10151347360649685No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATTGA10750.0294.96761
CACTGGA108.352242E-4294.96768
GATTGAC10750.0294.96762
ACTGGGT108.352242E-4294.96769
TGACACT10800.0293.6025
GACACTG10800.0293.6026
TTGACAC10800.0293.6024
ACACTGG10750.0293.595647
CACTGGG10650.0293.58288
ACTGGGA10550.0293.569649
ATTGACA10850.0292.2493
TGCCCCG452.5465852E-1159.2679130-34
CCCGCCG452.5465852E-1159.2679130-34
CCCCGCC452.5465852E-1159.2679130-34
ATGCCCC452.5465852E-1159.2679130-34
CCTAGCC900.059.2679130-34
GCCCCGC452.5465852E-1159.2679130-34
GCCTAGC850.059.26790630-34
CTTAGTT150.004280379559.26790630-34
TCAGCCC150.004280379559.26790630-34
Produced by FastQC (version 0.11.5)