FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-29-4-8-2-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-29-4-8-2-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12893
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA255319.801442643294813No Hit
CAACACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAC136110.556115721709455No Hit
CAACACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACAGATCTGT8516.600480881098271No Hit
CAACACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACAGATC6414.971690064376018No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA5023.8935856666408126No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAC3482.699139067711161No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA2752.132940355231521No Hit
CAACACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACAGATCTG2371.838206778872256No Hit
CAACACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACAGAT2341.8149383386333668No Hit
CAACACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA2181.690839990692624No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA2041.5822539362444736No Hit
CAACACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACAGATCT2021.5667416427518808No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA1941.5046924687815093No Hit
CAACACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT1871.4503994415574344No Hit
CAACACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTACAGAT1741.3495695338555804No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAC1561.2099588924222446No Hit
CAACACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC1311.0160552237648337No Hit
CAACACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACAGATC1230.9540060497944621No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA1180.9152253160629799No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA1140.8842007290777942No Hit
CAACACACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTACAGA1030.7988831148685334No Hit
CAACACACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTACA1010.7833708213759404No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAC940.7290777941518654No Hit
CAACACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC910.7058093539129761No Hit
CAACACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTACAGAT800.6204917397037152No Hit
CAACACACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTAC790.6127355929574188No Hit
CAACACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA750.581711005972233No Hit
CAACACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTACAG740.5739548592259366No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA710.5506864189870472No Hit
CAACACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTACA690.5351741254944544No Hit
CAACACACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTACA620.4808810982703793No Hit
CAACACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACAGAT610.4731249515240828No Hit
CAACACACTGGGATTAGATACCCCGAGCCACGAGACCTAGAGGAGCCTGT540.41883192430000776No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA540.41883192430000776No Hit
CAACACACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTA490.38005119056852554No Hit
CAACACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTACA460.35678275032963624No Hit
CAACACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA440.3412704568370434No Hit
CAACACACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTACAGATC440.3412704568370434No Hit
CAACACACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAGG440.3412704568370434No Hit
CAACACACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTACA430.3335143100907469No Hit
CAACACACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTACAGA430.3335143100907469No Hit
CAACACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTACAGATC430.3335143100907469No Hit
CAACACACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTACAGA390.30248972310556116No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA380.2947335763592647No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA350.2714651361203754No Hit
CAACACACTGGGATTAGATACCCCACGATACCTAGAGGAGCCTGTTCTAC350.2714651361203754No Hit
CAACACACTGGGATTAGATACCCCCGTAGTCCCTAGAGGAGCCTGTTCTA340.263708989374079No Hit
CAACACACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCT330.2559528426277825No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC330.2559528426277825No Hit
CAACACACTGGGATTAGATACCCCTAGTTGACTAGAGGAGCCTGTTCTAC320.24819669588148607No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG320.24819669588148607No Hit
CAACACACTGGGATTAGATACCCCATGCTAGAGGAGCCTGTTCTACAGAT310.24044054913518964No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA310.24044054913518964No Hit
CAACACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTACAGATC300.23268440238889318No Hit
CAACACACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAC290.22492825564259675No Hit
CAACACACTGGGATTAGATACCCCCCGAAATGGTCTAGAGGAGCCTGTTC280.21717210889630034No Hit
CAACACACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATAT240.18614752191111456No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA230.17839137516481812No Hit
CAACACACTGGGATTAGATACCCCTGACGCTGACTAGAGGAGCCTGTTCT230.17839137516481812No Hit
CAACACACTGGGATTAGATACCCCGACCCACCTAGAGGAGCCTGTTCTAC230.17839137516481812No Hit
CAACACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA220.1706352284185217No Hit
CAACACGCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA210.16287908167222523No Hit
CAACACACTGGGATTAGATACCCCGAGATCTAGAGGAGCCTGTTCTACAG190.14736678817963236No Hit
CAACACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT190.14736678817963236No Hit
CAACACACTGGGATTAGATACCCCTCCGAGCCCACGAGTCTAGAGGAGCC190.14736678817963236No Hit
CAACACACTGGGATTAGATACCCCAGCAGACCTAGAGGAGCCTGTTCTAC180.1396106414333359No Hit
CAACACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTACAGATCT180.1396106414333359No Hit
CAACACACTGGGATTAGATACCCCCGCAACCTAGAGGAGCCTGTTCTACA180.1396106414333359No Hit
CAACACACTGGGATTAGATACCCCCGTGGACTAGAGGAGCCTGTTCTACA170.1318544946870395No Hit
CAACACACTGGGATTAGATACCCCTTACTCTAGAGGAGCCTGTTCTACAG170.1318544946870395No Hit
CAACACACTGGGATTAGATACCCCAGACCCTAGAGGAGCCTGTTCTACAG170.1318544946870395No Hit
CAACACACTGGGATTAGATACCCCGACCCTAGAGGAGCCTGTTCTACAGA170.1318544946870395No Hit
CAACACACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTACAGA170.1318544946870395No Hit
CAACACACTGGGATTAGATACCCCGGTAGCTAGAGGAGCCTGTTCTACAG160.12409834794074304No Hit
CAACACGCTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAC160.12409834794074304No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTCAACA160.12409834794074304No Hit
CAACACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG150.11634220119444659No Hit
CAACACACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTACAG150.11634220119444659No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA150.11634220119444659No Hit
CAACACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG150.11634220119444659No Hit
CAACACACTGGGATTAGATACCCCAGTGCAGCTGTTGGAGACTAGAGGAG150.11634220119444659No Hit
CAACACACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTACAGATC150.11634220119444659No Hit
CAACACACTGGGATTAGATACCCCTGACTAGAGGAGCCTGTTCTACAGAT150.11634220119444659No Hit
CAACACACTGGGATTAGACACCCCACTATGCTTAGCCCTAAACCTCAACA140.10858605444815017No Hit
CAACACACTGGGATTAGATACCCCGTCAGACTAGAGGAGCCTGTTCTACA140.10858605444815017No Hit
CAACACACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCCT140.10858605444815017No Hit
CAACACACTGGGATTAGATACCCCAGACGCTGTCGATGACTAGAGGAGCC140.10858605444815017No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA130.10082990770185372No Hit
CAACACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT130.10082990770185372No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGATT157.0752594E-6295.08
ACTGGAT157.0752594E-6295.07
CGCTGGG108.3680527E-4294.999976
ACGCTGG108.3680527E-4294.999975
CACGCTG108.3680527E-4294.999974
GCTGGGA108.3680527E-4294.999977
ACACTGG12500.0292.639985
CTGGGAT12400.0292.620978
CACTGGG12350.0292.611336
ACTGGGA12300.0292.601627
TGGGATT12450.0291.445779
CACACTG12700.0289.192934
ACACACT13500.0273.148163
CAACACA15950.0233.040761
AACACAC16150.0230.15482
CACTGGA202.2301723E-5221.249986
TGGATTA202.2301723E-5221.249989
CAACACG301.12301524E-4147.51
AAACCTT501.8189894E-1259.00000440-44
AATCTTG303.2928074E-759.00000440-44
Produced by FastQC (version 0.11.5)