FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-28-4-4-1-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-28-4-4-1-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12598
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG213316.93125892998889No Hit
GGTACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTACC129610.287347197967932No Hit
GGTACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCTAGCTGTC11048.76329576123194No Hit
GGTACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACCTAGCT7025.572313065565963No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTACC5624.461025559612637No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG3622.873471979679314No Hit
GGTACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACCTAGCT2732.1670106366089854No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTACC2692.135259565010319No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAC2111.6748690268296555No Hit
GGTACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACCTAGCTGT2041.6193046515319893No Hit
GGTACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACCTAGCTG2021.6034291157326561No Hit
GGTACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACCTAGC1941.539926972535323No Hit
GGTACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACCTAGC1921.5240514367359899No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC1751.3891093824416574No Hit
GGTACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAC1671.3256072392443246No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTACC1561.2382917923479917No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG1531.2144784886489919No Hit
GGTACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT1331.0557231306556596No Hit
GGTACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTACCTA990.7858390220669947No Hit
GGTACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTACCTAGCT890.7064613430703286No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG770.6112081282743292No Hit
GGTACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTACCTAGCT760.6032703603746626No Hit
GGTACACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTACC680.5397682171773297No Hit
GGTACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTACCTAGC670.5318304492776631No Hit
GGTACACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTACCT630.5000793776789967No Hit
GGTACACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTACCTAG620.4921416097793301No Hit
GGTACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT610.4842038418796634No Hit
GGTACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTACCT600.4762660739799968No Hit
GGTACACTGGGATTAGATACCCCTCTTATCACGTCTGACGCTAGAGGAGC600.4762660739799968No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG570.45245277028099695No Hit
GGTACACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTACCTAG570.45245277028099695No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCTAGAGGAGCCTGTTCTACCT570.45245277028099695No Hit
GGTACACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTACCTAGCT550.43657723448166375No Hit
GGTACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG520.4127639307826639No Hit
GGTACACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTACC520.4127639307826639No Hit
GGTACACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTACCTAG460.36513732338466426No Hit
GGTACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTACCT450.3571995554849976No Hit
GGTACACTGGGATTAGATACCCCACGGACCTAGAGGAGCCTGTTCTACCT440.349261787585331No Hit
GGTACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTACCTAGC440.349261787585331No Hit
GGTACACTGGGATTAGATACCCCTCGAGACCTAGAGGAGCCTGTTCTACC430.3413240196856644No Hit
GGTACACTGGGATTAGATACCCCTGACCCTAGAGGAGCCTGTTCTACCTA430.3413240196856644No Hit
GGTACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTACCT420.3333862517859978No Hit
GGTACACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTAG420.3333862517859978No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT410.32544848388633113No Hit
GGTACACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTACCTAGCTG410.32544848388633113No Hit
GGTACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCTA380.3016351801873313No Hit
GGTACACTGGGATTAGATACCCCACTAACACTAGAGGAGCCTGTTCTACC360.2857596443879981No Hit
GGTACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG360.2857596443879981No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG350.2778218764883315No Hit
GGTACACTGGGATTAGATACCCCTGACTAGAGGAGCCTGTTCTACCTAGC350.2778218764883315No Hit
GGTACACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTAC350.2778218764883315No Hit
GGTACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTACCT330.26194634068899825No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCTAGAGGAGCCTGTTCTACCT330.26194634068899825No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAC320.25400857278933164No Hit
GGTACACTGGGATTAGATACCCCTGAGAACTAGAGGAGCCTGTTCTACCT320.25400857278933164No Hit
GGTACACTGGGATTAGATACCCCCACACCTAGAGGAGCCTGTTCTACCTA310.24607080488966504No Hit
GGTACACTGGGATTAGATACCCCCAAGACCTAGAGGAGCCTGTTCTACCT310.24607080488966504No Hit
GGTACACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTACC300.2381330369899984No Hit
GGTACACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTACCTAG300.2381330369899984No Hit
GGTACACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTACC300.2381330369899984No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG290.2301952690903318No Hit
GGTACACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTACCT280.2222575011906652No Hit
GGTACACTGGGATTAGATACCCCATGTCAGACTAGAGGAGCCTGTTCTAC280.2222575011906652No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG280.2222575011906652No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG270.21431973329099854No Hit
GGTACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTACCTAGCT270.21431973329099854No Hit
GGTACACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTACCTA260.20638196539133194No Hit
GGTACACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTACCTAG260.20638196539133194No Hit
GGTACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG250.19844419749166534No Hit
GGTACACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTACCTAGCT240.19050642959199873No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAC230.18256866169233213No Hit
GGTACACTGGGATTAGATACCCCTGTATAAGAGGAGCCTGTTCTACCTAG230.18256866169233213No Hit
GGTACACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTACCTAGCT210.1666931258929989No Hit
GGTACACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCCTA210.1666931258929989No Hit
GGTACACTGGGATTAGATACCCCTATAAAGGGCTAGAGGAGCCTGTTCTA200.15875535799333226No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG190.15081759009366566No Hit
GGTACACTGGGATTAGATACCCCCGTGGACTAGAGGAGCCTGTTCTACCT190.15081759009366566No Hit
GGTACACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTACCTAG190.15081759009366566No Hit
GGTACACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATATC180.14287982219399906No Hit
GGTACACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAGGT180.14287982219399906No Hit
GGTACACTGGGATTAGATACCCCGCACGGACCTAGAGGAGCCTGTTCTAC170.13494205429433243No Hit
GGTACACTGGGATTAGATACCCCTGAGCCCACGAGACCTAGAGGAGCCTG160.12700428639466582No Hit
GGTACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT160.12700428639466582No Hit
GGTACACTGGGATTAGATACCCCATCTCCGAGCCCACGAACCTAGAGGAG160.12700428639466582No Hit
GGTACACTGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTACCT160.12700428639466582No Hit
GGTACACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTACCTAG150.1190665184949992No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG140.1111287505953326No Hit
GGTACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT130.10319098269566597No Hit
GGTACACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTACCTAG130.10319098269566597No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGAT108.3657133E-4295.06
GGTACAC12450.0293.815251
CACTGGG12150.0293.785985
ACTGGGA12100.0293.780986
TACACTG12450.0292.630523
ACACTGG12400.0292.620974
CTGGGAT12200.0292.581977
GGGATTA12150.0292.5729
TGGGATT12150.0292.5728
GTACACT12600.0290.317442
CACTGGA202.228925E-5221.255
CTGGATT150.0028163472196.666677
GCCCCAA303.2915523E-759.000004115-119
AGTAGTT150.004361260759.00000445-49
ACGTCTG150.004361260759.00000430-34
GTTCTAG150.004361260759.000004145-149
CCGCCAG1200.059.00000465-69
GTCTGAC150.004361260759.00000430-34
TAGTTAC150.004361260759.00000445-49
CTAGGAG150.004361260759.00000480-84
Produced by FastQC (version 0.11.5)