FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-27-4-2-6-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-27-4-2-6-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12762
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA197315.459959254035418No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAC131910.335370631562451No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCACTCCTG12129.496944052656325No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACCACTC6194.850336937784046No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA3993.1264692054536907No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAC2982.3350572010656636No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACCACTCCT2802.194013477511362No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACCACT2712.123491615734211No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA2401.8805829807240242No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACCACT2391.8727472183043408No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAC2301.80222535652719No Hit
GCCACAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACCACTC2071.622002820874471No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA1951.52797367183827No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT1751.371258423444601No Hit
GCCACAACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTACC1671.3085723240871336No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACCACTCC1531.1988716502115657No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA1431.1205140260147313No Hit
GCCACAACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA1351.0578279266572637No Hit
GCCACAACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTACC1090.8540981037454944No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA1080.846262341325811No Hit
GCCACAACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTACCACT1030.8070835292273938No Hit
GCCACAACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTACC1020.7992477668077104No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC990.7757404795486601No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA940.7365616674502429No Hit
GCCACAACTGGGATTAGATACCCCCGTACGTGCAGGGAGGGTACCTTGAG920.720890142610876No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAC890.6973828553518256No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA800.6268609935746748No Hit
GCCACAACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTACC790.6190252311549914No Hit
GCCACAACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTA790.6190252311549914No Hit
GCCACAACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTACCACT780.611189468735308No Hit
GCCACAACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTACCA660.5171603196991067No Hit
GCCACAACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTT640.5014887948597399No Hit
GCCACAACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTAC630.4936530324400564No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCTA550.43096693308258893No Hit
GCCACAACTGGGATTAGATACCCCCGAGCTAGAGGAGCCTGTTCTACCAC530.41529540824322203No Hit
GCCACAACTGGGATTAGATACCCCGCCAACTAGAGGAGCCTGTTCTACCA530.41529540824322203No Hit
GCCACAACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCCT520.4074596458235386No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA510.3996238834038552No Hit
GCCACAACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTACCACTC490.38395235856448834No Hit
GCCACAACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTAC490.38395235856448834No Hit
GCCACAACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTACCAC480.37611659614480486No Hit
GCCACAACTGGGATTAGATACCCCTATAGTCCTAGAGGAGCCTGTTCTAC470.36828083372512144No Hit
GCCACAACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTACCACTC450.3526093088857546No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA440.3447735464660711No Hit
GCCACAACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTACCAC430.3369377840463877No Hit
GCCACAACTGGGATTAGATACCCCCAGTAGAGGAGCCTGTTCTACCACTC400.3134304967873374No Hit
GCCACAACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTACCACTC400.3134304967873374No Hit
GCCACAACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTACCAC390.305594734367654No Hit
GCCACAACTGGGATTAGATACCCCCGGACCTAGAGGAGCCTGTTCTACCA390.305594734367654No Hit
GCCACAACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGTT390.305594734367654No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTACC370.2899232095282871No Hit
GCCACAACTGGGATTAGATACCCCGAGCCACCTAGAGGAGCCTGTTCTAC360.2820874471086037No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA360.2820874471086037No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG360.2820874471086037No Hit
GCCACAACTGGGATTAGATACCCCTGACGCTGCTAGAGGAGCCTGTTCTA350.27425168468892025No Hit
GCCACAACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAC350.27425168468892025No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA350.27425168468892025No Hit
GCCACAACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTACC300.23507287259050302No Hit
GCCACAACTGGGATTAGATACCCCAGTGCAGCTGTTGGAGACTAGAGGAG300.23507287259050302No Hit
GCCACAACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCT300.23507287259050302No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA290.2272371101708196No Hit
GCCACAACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTACCAC290.2272371101708196No Hit
GCCACAACTGGGATTAGATACCCCATCTCCGAGCCCACGAACCTAGAGGA290.2272371101708196No Hit
GCCACAACTGGGATTAGATACCCCGGCCCACGAGACCTAGAGGAGCCTGT290.2272371101708196No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA280.21940134775113618No Hit
GCCACAACTGGGATTAGATACCCCCGACCAACCTAGAGGAGCCTGTTCTA280.21940134775113618No Hit
GCCACAACTGGGATTAGATACCCCGAGCCTAGAGGAGCCTGTTCTACCAC270.21156558533145275No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCTAGAGGAGCCTGTTCTACCA250.19589406049208588No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC240.18805829807240243No Hit
GCCACAACTGGGATTAGATACCCCACTAACACTAGAGGAGCCTGTTCTAC240.18805829807240243No Hit
GCCACAACTGGGATTAGATACCCCGTCTCTTATACCATCTGAGCTAGAGG230.180222535652719No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA230.180222535652719No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA230.180222535652719No Hit
GCCACAACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTACCACTC220.17238677323303556No Hit
GCCACAACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTACCAC210.16455101081335213No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT210.16455101081335213No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA190.1488794859739853No Hit
GCCACAACTGGGATTAGATACCCCTCTCCGAACCCACGAGACCTAGAGGA190.1488794859739853No Hit
GCCACAACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTACCAC180.14104372355430184No Hit
GCCACAACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC170.1332079611346184No Hit
GCCACAACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTACCACT170.1332079611346184No Hit
GCCACAACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATA160.12537219871493496No Hit
GCCACAACTGGGATTAGATACCCCCCACGAGACCTAGAGGAGCCTGTTCT150.11753643629525151No Hit
GCCACAACTGGGATTAGATACCCCAGAGGAGCCTGTTCTACCACTCCTGT150.11753643629525151No Hit
GCCACAACTGGGATTAGATACCCCGACGACCTAGAGGAGCCTGTTCTACC150.11753643629525151No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA140.10970067387556809No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA130.10186491145588465No Hit
GCCACAACTGGGATTAGATACCTCTGTAGTCCTAGAGGAGCCTGTTCTAC130.10186491145588465No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGGG12550.0295.06
CTGGGAT12550.0295.08
CAACTGG12600.0295.05
CTAGGAT108.3672843E-4295.08
ACTGGGA12550.0295.07
GCCACAA12750.0293.843141
TGGGATT12600.0292.658759
ACAACTG13100.0283.740454
CCACAAC13250.0282.75472
CACAACT13450.0278.55023
TAGGATT150.0028168755196.666669
AACTAGG150.0028168755196.666666
ACTAGGA200.006660292147.57
AACGCTT257.750024E-659.00000490-94
TTCATAT2500.059.000004120-124
CCCAGTA452.5465852E-1159.00000420-24
CCAGTAG452.5465852E-1159.00000420-24
CCGCCAG900.059.00000465-69
CGCTTAA257.750024E-659.00000490-94
AACCTTG501.8189894E-1259.00000440-44
Produced by FastQC (version 0.11.5)