FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-26-5-8-2-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-26-5-8-2-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12559
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA443535.31332112429333No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA8927.102476311808265No Hit
CAACACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAA6575.231308225177163No Hit
CAACACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAACTTCGCCT4853.861772434110996No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA3632.8903575125408074No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA3162.5161238952145872No Hit
CAACACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAACTTCG2281.8154311649016641No Hit
CAACACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAACTTC2051.6322955649335138No Hit
CAACACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT2041.6243331475435945No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAA1951.552671391034318No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA1881.496934469304881No Hit
CAACACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC1481.1784377737080978No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG1381.098813599808902No Hit
CAACACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAACTTCGCC1281.019189425909706No Hit
CAACACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAACTTCGC1160.9236404172306713No Hit
CAACACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAACTTCG1050.836053825941556No Hit
CAACACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA1020.8121665737717972No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA990.7882793216020383No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAA840.6688430607532446No Hit
CAACACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA810.6449558085834859No Hit
CAACACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA630.5016322955649335No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA630.5016322955649335No Hit
CAACACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA620.4936698781750139No Hit
CAACACACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAAC600.47774504339517476No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC590.4697826260052552No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA560.4458953738354965No Hit
CAACACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAAC550.4379329564455769No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA530.42200812166573776No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAA500.398120869495979No Hit
CAACACACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTAA490.39015845210605943No Hit
CAACACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAACTTC480.38219603471613983No Hit
CAACACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAAC480.38219603471613983No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA450.3583087825463811No Hit
CAACACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAAC420.3344215303766223No Hit
CAACACACTGGGATTAGATACCCCACTGACACTAGAGGAGCCTGTTCTAA420.3344215303766223No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA410.32645911298670277No Hit
CAACACACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAA400.3184966955967832No Hit
CAACACACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTA400.3184966955967832No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA370.29460944342702444No Hit
CAACACGCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA370.29460944342702444No Hit
CAACACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC330.2627597738673461No Hit
CAACACACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTA330.2627597738673461No Hit
CAACACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT320.2547973564774265No Hit
CAACACACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTAA320.2547973564774265No Hit
CAACACACTGGGATTAGATACCCCTGAGCCCACGAGACCTAGAGGAGCCT300.23887252169758738No Hit
CAACACACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAACTT290.2309101043076678No Hit
CAACACACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTAACT280.22294768691774824No Hit
CAACACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAACTTCG260.20702285213790908No Hit
CAACACACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCT250.1990604347479895No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA250.1990604347479895No Hit
CAACACACTGGGATTAGATACCCCAATTAATACGACTCACTAGAGGAGCC250.1990604347479895No Hit
CAACACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT240.19109801735806992No Hit
CAACACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA230.18313559996815035No Hit
CAACACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAACTTC220.17517318257823075No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA220.17517318257823075No Hit
CAACACACTGGGATTAGATACCCCTGTATAAGAGGAGCCTGTTCTAACTT210.16721076518831116No Hit
CAACACACTGGGATTAGACACCCCACTATGCTTAGCCCTAAACCTCAACA200.1592483477983916No Hit
CAACACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAACT190.15128593040847202No Hit
CAACACACTGGGATTAGATACCCCAGCCCACGAGACCTAGAGGAGCCTGT190.15128593040847202No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA190.15128593040847202No Hit
CAACACACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCCT180.14332351301855245No Hit
CAACACACTGGGATTAGATACCCCGTAGTCCTAGAGGAGCCTGTTCTAAC170.13536109562863285No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA170.13536109562863285No Hit
CAACACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG160.12739867823871326No Hit
CAACACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGC160.12739867823871326No Hit
CAACACACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTAA150.11943626084879369No Hit
CAACACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG150.11943626084879369No Hit
CAACACACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAGG150.11943626084879369No Hit
CAACACACTGGGATTAGATACCCCAATGAGATCGAGTGCCTAGAGGAGCC140.11147384345887412No Hit
CAACACACTGGGATTAGATACCCCCGGTAGTCCTAGAGGAGCCTGTTCTA140.11147384345887412No Hit
CAACACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAACTTCG140.11147384345887412No Hit
CAACACACTGGGATTAGATACCCCAGGACCTAGAGGAGCCTGTTCTAACT130.10351142606895454No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAACA130.10351142606895454No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATA130.10351142606895454No Hit
CAACACACTGGGATTAGATACCCCAAGCCCACGAGACCTAGAGGAGCCTG130.10351142606895454No Hit
CAACACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATA130.10351142606895454No Hit
CAACACACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAACTTCG130.10351142606895454No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGGG108.370452E-4294.944245
CACACGG108.370452E-4294.944244
ACACTGG12200.0294.944245
CGGGATT108.370452E-4294.944248
ACGGGAT108.370452E-4294.944247
CACGGGA108.370452E-4294.944246
CACTGGG12100.0294.94426
CACACTG12250.0293.740364
ACTGGGA12200.0292.526647
CTGGGAT12350.0291.36198
TGGGATT12300.0291.347359
ACACACT12850.0280.024873
CAACACA15200.0238.671951
AACACAC15850.0228.884192
AACTGGG150.002817942196.629475
CAACTGG150.002817942196.629474
CACTGGA150.002817942196.629476
AGCCCTA5550.059.22480430-34
CAGCCCT150.00429600259.224830-34
GCCTAGC1650.059.224830-34
Produced by FastQC (version 0.11.5)