FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-25-4-4-1-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-25-4-4-1-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12356
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG218917.71608934930398No Hit
GGTACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAGG164913.345742958886369No Hit
GGTACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGGAATCGCTG11909.630948527031402No Hit
GGTACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGGAATCG7646.183230819035287No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG3372.727419876982842No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAGG2602.1042408546455165No Hit
GGTACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGGAATCGCT2552.063774684363872No Hit
GGTACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGGAATC2411.950469407575267No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAGG2221.7966979605050177No Hit
GGTACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGGAATCG1991.6105535772094528No Hit
GGTACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGGAATCGC1721.3920362576885723No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC1691.3677565555195856No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG1521.2301715765619943No Hit
GGTACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT1341.0844933635480738No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAG1251.0116542570411136No Hit
GGTACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGGAATCG1220.987374554872127No Hit
GGTACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAGGA1110.8983489802525089No Hit
GGTACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGGAATC1040.8416963418582066No Hit
GGTACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAGGA980.7931369375202332No Hit
GGTACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGGAATC970.7850437034639042No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG860.6960181288442862No Hit
GGTACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAG850.6879248947879573No Hit
GGTACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGGAA830.6717384266752995No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAG720.5827128520556815No Hit
GGTACACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAGGA710.5746196179993526No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAG660.534153447717708No Hit
GGTACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCTA620.5017805114923923No Hit
GGTACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGGAATC600.48559404337973455No Hit
GGTACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGGAATCG580.46940757526707677No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG550.44512787309809004No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAGG550.44512787309809004No Hit
GGTACACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTAGGAA530.4289414049854322No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTAGGA500.4046617028164455No Hit
GGTACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG450.3641955325348009No Hit
GGTACACTGGGATTAGATACCCCAAACCTAGAGGAGCCTGTTCTAGGAAT430.3480090644221431No Hit
GGTACACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTAGG430.3480090644221431No Hit
GGTACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGGA420.3399158303658142No Hit
GGTACACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAGG420.3399158303658142No Hit
GGTACACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTAG380.30754289414049857No Hit
GGTACACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTAGG370.2994496600841696No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG370.2994496600841696No Hit
GGTACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTCT360.29135642602784073No Hit
GGTACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGGAATCGC350.28326319197151184No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG340.2751699579151829No Hit
GGTACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG310.25089025574619617No Hit
GGTACACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTAGGAAT300.24279702168986728No Hit
GGTACACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAGGA300.24279702168986728No Hit
GGTACACTGGGATTAGATACCCCCGACCAACCTAGAGGAGCCTGTTCTAG300.24279702168986728No Hit
GGTACACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAGGAATC290.23470378763353839No Hit
GGTACACTGGGATTAGATACCCCTGACGTCTAGAGGAGCCTGTTCTAGGA290.23470378763353839No Hit
GGTACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT290.23470378763353839No Hit
GGTACACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCTG290.23470378763353839No Hit
GGTACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGGAATCG290.23470378763353839No Hit
GGTACACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTAGGAATCGC290.23470378763353839No Hit
GGTACACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTAGG290.23470378763353839No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG280.22661055357720947No Hit
GGTACACTGGGATTAGATACCCCACGAGGCCTAGAGGAGCCTGTTCTAGG250.20233085140822274No Hit
GGTACACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCCTA240.19423761735189382No Hit
GGTACACTGGGATTAGATACCCCACCCTAGAGGAGCCTGTTCTAGGAATC230.1861443832955649No Hit
GGTACACTGGGATTAGATACCCCACTAGACCTAGAGGAGCCTGTTCTAGG230.1861443832955649No Hit
GGTACACTGGGATTAGATACCCCAGCTAGAGGAGCCTGTTCTAGGAATCG220.178051149239236No Hit
GGTACACTGGGATTAGATACCCCCACGAGACTAGAGGAGCCTGTTCTAGG220.178051149239236No Hit
GGTACACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAGG220.178051149239236No Hit
GGTACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGGAATCG220.178051149239236No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG220.178051149239236No Hit
GGTACACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTAGGAATC220.178051149239236No Hit
GGTACACTGGGATTAGATACCCCTATAAGAGGAGCCTGTTCTAGGAATCG220.178051149239236No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG220.178051149239236No Hit
GGTACACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTCTA220.178051149239236No Hit
GGTACACTGGGATTAGATACCCCAGATCTAGAGGAGCCTGTTCTAGGAAT210.1699579151829071No Hit
GGTACACTGGGATTAGATACCCCACGGACCTAGAGGAGCCTGTTCTAGGA210.1699579151829071No Hit
GGTACACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAGGAAT210.1699579151829071No Hit
GGTACACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTAGGAAT190.15377144707024928No Hit
GGTACACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGGAAT190.15377144707024928No Hit
GGTACACTGGGATTAGATACCCCGTGTAGTCCCTAGAGGAGCCTGTTCTA190.15377144707024928No Hit
GGTACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT180.14567821301392037No Hit
GGTACACTGGGATTAGATACCCCCAGAACCTAGAGGAGCCTGTTCTAGGA180.14567821301392037No Hit
GGTACACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTAGGA180.14567821301392037No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT180.14567821301392037No Hit
GGTACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT180.14567821301392037No Hit
GGTACACTGGGATTAGATACCCCAGTAGCTAGAGGAGCCTGTTCTAGGAA170.13758497895759145No Hit
GGTACACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAGGAA170.13758497895759145No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTCG160.12949174490126256No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG160.12949174490126256No Hit
GGTACACTGGGATTAGATACCCCGAGCCACGAGACCTAGAGGAGCCTGTT160.12949174490126256No Hit
GGTACACTGGGATTAGATACCCCGTATAGAGGAGCCTGTTCTAGGAATCG160.12949174490126256No Hit
GGTACACTGGGATTAGATACCCCCTGTCTCCCTAGAGCTAGAGGAGCCTG150.12139851084493364No Hit
GGTACACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTAGGAA150.12139851084493364No Hit
GGTACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG150.12139851084493364No Hit
GGTACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT140.11330527678860473No Hit
GGTACACTGGGATTAGATACCCCGGGTAGTCTAGAGGAGCCTGTTCTAGG140.11330527678860473No Hit
GGTACACTGGGATTAGATACCCCGCCCGAGACCTAGAGGAGCCTGTTCTA140.11330527678860473No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG140.11330527678860473No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGAT12150.0295.07
ACACTGG12250.0295.04
CACTGGG12150.0295.05
CTGGATT108.364092E-4295.07
TGGATTA108.364092E-4295.08
ACTGGGA12150.0295.06
TGGGATT12150.0293.7868
GGTACAC12450.0292.630521
GGGATTA12200.0292.581979
GTACACT12450.0291.445772
TACACTG12500.0290.283
CACTGGA150.0028158033196.666665
ACTGGAT150.0028158033196.666666
GCCCTAA3300.059.00000430-34
TAGTCCC452.5465852E-1159.00000425-29
CCCGTCT900.059.00000420-24
TCGCCAG2750.059.00000465-69
CGGTGCT2750.059.000004110-114
ACCCCCA452.5465852E-1159.00000415-19
CTAAACT452.5465852E-1159.00000435-39
Produced by FastQC (version 0.11.5)