FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-24-5-8-2-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-24-5-8-2-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12418
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA332626.78370107907876No Hit
CAACACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG10308.294411338379772No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA8056.482525366403608No Hit
CAACACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGACGTGGCT6014.839748751811886No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAG4703.7848284747946526No Hit
CAACACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGACGTG3362.705749718151071No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA3242.609115799645676No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA3062.4641649218875825No Hit
CAACACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC2451.9729425028184893No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG2421.9487840231921405No Hit
CAACACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGACGTGGC1991.6025124818811403No Hit
CAACACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT1911.53808986954421No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAG1881.5139313899178612No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA1491.1998711547753262No Hit
CAACACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGACGTG1261.0146561443066515No Hit
CAACACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGACGTGG1160.9341278788854888No Hit
CAACACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGACGT1060.8535996134643261No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA1060.8535996134643261No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAG1020.8213883072958608No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA870.7005959091641166No Hit
CAACACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA870.7005959091641166No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC850.684490256079884No Hit
CAACACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGACGTG770.6200676437429538No Hit
CAACACACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTA730.5878563375744886No Hit
CAACACACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAG690.5556450314060235No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA650.5234337252375584No Hit
CAACACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGACGT640.5153808986954421No Hit
CAACACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGACGT540.43485263327427925No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA470.3784828474794653No Hit
CAACACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAGA470.3784828474794653No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA420.3382187147688839No Hit
CAACACACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAGACG410.33016588822676757No Hit
CAACACACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAGACGTG400.3221130616846513No Hit
CAACACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGACGTG400.3221130616846513No Hit
CAACACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA400.3221130616846513No Hit
CAACACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGAC390.31406023514253506No Hit
CAACACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGACGT360.2899017555161862No Hit
CAACACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA360.2899017555161862No Hit
CAACACACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTAGA350.2818489289740699No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA330.2657432758898373No Hit
CAACACACTGGGATTAGATACCCCGGGTAGTCTAGAGGAGCCTGTTCTAG320.25769044934772106No Hit
CAACACACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCT310.24963762280560478No Hit
CAACACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGACGTG300.2415847962634885No Hit
CAACACACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAGACGTG270.21742631663713963No Hit
CAACACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTAGA270.21742631663713963No Hit
CAACACACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGTT270.21742631663713963No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA240.19326783701079078No Hit
CAACACACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTAG240.19326783701079078No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA240.19326783701079078No Hit
CAACACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAGA240.19326783701079078No Hit
CAACACACTGGGATTAGATACCCCTAGTTGACTAGAGGAGCCTGTTCTAG230.1852150104686745No Hit
CAACACACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAGACGT230.1852150104686745No Hit
CAACACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGC230.1852150104686745No Hit
CAACACACTGGGATTAGACACCCCACTATGCTTAGCCCTAAACCTCAACA210.16910935738444194No Hit
CAACACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT210.16910935738444194No Hit
CAACACACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAGACG210.16910935738444194No Hit
CAACACACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTAG200.16105653084232566No Hit
CAACACACTGGGATTAGATACCCCGAGCCACGAGACCTAGAGGAGCCTGT200.16105653084232566No Hit
CAACACACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATAT200.16105653084232566No Hit
CAACACGCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA190.15300370430020938No Hit
CAACACACTGGGATTAGATACCCGAGTAGTCCTAGAGGAGCCTGTTCTAG190.15300370430020938No Hit
CAACACACTGGGATTAGATACCCCAACGAGACCTAGAGGAGCCTGTTCTA190.15300370430020938No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACTAGAGGAGCCTGT180.1449508777580931No Hit
CAACACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAGACGTG180.1449508777580931No Hit
CAACACACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTAG160.12884522467386053No Hit
CAACACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGA160.12884522467386053No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT160.12884522467386053No Hit
CAACACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT150.12079239813174425No Hit
CAACACACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAGAC140.11273957158962795No Hit
CAACACACTGGGATTAGATACCCCTGACCCTAGAGGAGCCTGTTCTAGAC140.11273957158962795No Hit
CAACACACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAG140.11273957158962795No Hit
CAACACACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAGA130.10468674504751167No Hit
CAACACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG130.10468674504751167No Hit
CAACACACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAGA130.10468674504751167No Hit
CAACACACTGGGATTAGATACCCCACGACCTAGAGGAGCCTGTTCTAGAC130.10468674504751167No Hit
CAACACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA130.10468674504751167No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTC130.10468674504751167No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTGG12200.0295.05
CACTGGG12100.0295.06
CACTGGA108.364502E-4295.06
ACCTGGG108.364502E-4295.05
CACCTGG108.364502E-4295.04
CTGGATT108.364502E-4295.08
ACTGGGA12100.0295.07
ACTGGAT108.364502E-4295.07
CTGGGAT12250.0292.591838
TGGGATT12250.0292.591839
CACACTG12400.0290.241944
ACACACT12800.0281.171883
AACACAC14850.0243.350162
CAACACA15150.0238.531371
ACACCTG150.0028159407196.666673
CCTGGGA150.0028159407196.666676
TGGATTA200.006658088147.59
CAATGTG150.004361015359.000004255-259
TTCACAT150.004361015359.000004120-124
TAAACTC257.747518E-659.00000435-39
Produced by FastQC (version 0.11.5)