FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-23-4-8-2-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-23-4-8-2-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12563
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA242819.3265939664093No Hit
CAACACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG154512.298017989333758No Hit
CAACACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGGAATCGCT11939.496139457136033No Hit
CAACACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGGAATC6415.102284486189604No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAG4203.3431505213722836No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA4143.295391228209822No Hit
CAACACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGGAATCGC3042.419804186898034No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAG2381.8944519621109608No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA2201.751174082623577No Hit
CAACACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGGAAT2151.7113746716548595No Hit
CAACACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT1711.3611398551301441No Hit
CAACACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAGG1581.257661386611478No Hit
CAACACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC1531.2178619756427604No Hit
CAACACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGGAATCG1521.209902093449017No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA1421.1303032715115817No Hit
CAACACACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTAGGA1301.0347846851866591No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAG1281.0188649207991722No Hit
CAACACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGGAAT1100.8755870413117885No Hit
CAACACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC1080.8596672769243014No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA1050.8357876303430709No Hit
CAACACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGGAATC950.7561888084056356No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA890.7084295152431744No Hit
CAACACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA820.6527103398869697No Hit
CAACACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGGAATC760.6049510467245085No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA710.5651516357557909No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA700.5571917535620472No Hit
CAACACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA680.5412719891745602No Hit
CAACACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGGAAT620.493512696012099No Hit
CAACACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGGA580.46167316723712487No Hit
CAACACACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGGAATC580.46167316723712487No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA580.46167316723712487No Hit
CAACACACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCTA570.4537132850433814No Hit
CAACACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGG500.39799410968717663No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA420.33431505213722834No Hit
CAACACACTGGGATTAGATACCCCCACACCTAGAGGAGCCTGTTCTAGGA410.32635516994348485No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC410.32635516994348485No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA410.32635516994348485No Hit
CAACACACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTA370.2945156411685107No Hit
CAACACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA340.2706359945872801No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTAGG340.2706359945872801No Hit
CAACACACTGGGATTAGATACCCCACGACCTAGAGGAGCCTGTTCTAGGA340.2706359945872801No Hit
CAACACACTGGGATTAGATACCCCCGCAACCTAGAGGAGCCTGTTCTAGG330.2626761123935366No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG320.25471623019979306No Hit
CAACACACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAGGAA310.2467563480060495No Hit
CAACACACTGGGATTAGATACCCCTATAAAGGGCTAGAGGAGCCTGTTCT300.23879646581230599No Hit
CAACACACTGGGATTAGATACCCCTCAGACTAGAGGAGCCTGTTCTAGGA300.23879646581230599No Hit
CAACACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGGAATC260.20695693703733187No Hit
CAACACACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTT250.19899705484358832No Hit
CAACACACTGGGATTAGATACCCCTGACGCTACTAGAGGAGCCTGTTCTA240.1910371726498448No Hit
CAACACACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTAGGAA240.1910371726498448No Hit
CAACACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGGAAT240.1910371726498448No Hit
CAACACACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAG220.17511740826235772No Hit
CAACACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA220.17511740826235772No Hit
CAACACACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAGGA210.16715752606861417No Hit
CAACACACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTAG210.16715752606861417No Hit
CAACACACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTA210.16715752606861417No Hit
CAACACGCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA200.15919764387487065No Hit
CAACACACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTAGGA200.15919764387487065No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA190.15123776168112713No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA190.15123776168112713No Hit
CAACACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT180.14327787948738357No Hit
CAACACACTGGGATTAGATACCCCGCCAAACCTAGAGGAGCCTGTTCTAG180.14327787948738357No Hit
CAACACACTGGGATTAGATACCCCGTCAGACTAGAGGAGCCTGTTCTAGG180.14327787948738357No Hit
CAACACACTGGGATTAGATACCCCAATTAATACGACTCACTAGAGGAGCC170.13531799729364005No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA170.13531799729364005No Hit
CAACACACTGGGATTAGATACCCCTCCGAGCCCAGAACCTAGAGGAGCCT170.13531799729364005No Hit
CAACACACTGGGATTAGATACCCCTCAGAGCCCACGAGACCTAGAGGAGC160.12735811509989653No Hit
CAACACACTGGGATTAGATACCCCCCGAGACCTAGAGGAGCCTGTTCTAG160.12735811509989653No Hit
CAACACACTGGGATTAGACACCCCACTATGCTTAGCCCTAAACCTCAACA150.11939823290615299No Hit
CAACACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT150.11939823290615299No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA140.11143835071240946No Hit
CAACACGCTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG140.11143835071240946No Hit
CAACACACTGGGATTAGATACCCCGATCTAGAGGAGCCTGTTCTAGGAAT140.11143835071240946No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT130.10347846851866593No Hit
CAACACACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTAGGAA130.10347846851866593No Hit
CAACACACTGGGATTAGATACCCCCGTCTAGAGGAGCCTGTTCTAGGAAT130.10347846851866593No Hit
CAACACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG130.10347846851866593No Hit
CAACACACTGGGATTAGATACCCCGGTAGCTAGAGGAGCCTGTTCTAGGA130.10347846851866593No Hit
CAACACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATA130.10347846851866593No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGG157.071296E-6295.06
ACGCTGG157.071296E-6295.05
CACGCTG157.071296E-6295.04
ACACTGG12300.0295.05
CACTGGG12250.0295.06
TGGGATA157.071296E-6295.09
AACACGC157.071296E-6295.02
CTGGGAT12400.0293.81058
TGGGATT12250.0293.795939
ACTGGGA12250.0293.795937
CACACTG12450.0292.630524
ACACACT13400.0271.884343
CAACACA15500.0235.04841
AACACAC15500.0235.04842
ACACGCT202.228925E-5221.253
GCTGGGA202.228925E-5221.257
CAACACG301.12238966E-4147.51
GGAGATC150.004361260759.00000425-29
CCTATAA150.004361260759.00000420-24
CCCAGAG150.004361260759.00000420-24
Produced by FastQC (version 0.11.5)