FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-220-6-5-8-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-220-6-5-8-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621115
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA28755046.29577453450649No Hit
TGATTGACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGGAATCG275724.439113529700619No Hit
TGATTGACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCA245963.9599752058797484No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAA196143.1578693156661806No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAA150262.4191977331090055No Hit
TGATTGACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAA148882.3969796253511833No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGA136132.1917036297626042No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAA121791.9608285100182736No Hit
TGATTGACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAA119521.924281332764464No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAA97961.577163649243699No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAA95861.5433534852644035No Hit
TGATTGACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAA94001.5134073400255992No Hit
TGATTGACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGGAATC69211.1142864042890608No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA68061.0957713144908752No Hit
TGATTGACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGGAA64611.0402260450963188No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGA54360.8752002447211868No Hit
TGATTGACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGGAA53670.8640911908422757No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGA48660.7834297996345283No Hit
TGATTGACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAA48060.773769752783301No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAGA47830.7700667348236638No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGA36400.5860428423077853No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCT35270.5678497540713072No Hit
TGATTGACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCC34170.5501396681773907No Hit
TGATTGACACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTAGGAAT27810.447743171554382No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAA27410.44130314032023055No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTA25280.4070099739983739No Hit
TGATTGACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTA24950.40169694823019897No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGA24730.3981549310514156No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTAG18420.2965634383326759No Hit
TGATTGACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC17910.2883523985091328No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGA15710.2529322267212996No Hit
TGATTGACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAG15420.2482632040765398No Hit
TGATTGACACTGGGATTAGATACCCCCAGCGATTCCTAGAGGAGCCTGTT14170.2281381064698164No Hit
TGATTGACACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAGGAA13720.22089307133139596No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAG13370.2152580440015134No Hit
TGATTGACACTGGGATTAGATACCCCTAGGATTGTTGGCAAGCTAGAGGA13350.21493604243980582No Hit
TGATTGACACTGGGATTAGATACCCCTGTCTCGTGGTCTAGAGGAGCCTG12670.20398798934174828No Hit
TGATTGACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGGAAT12630.20334398621833313No Hit
TGATTGACACTGGGATTAGATACCCCACTGACACTAGAGGAGCCTGTTCT11950.19239593312027561No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAA11570.18627790344783174No Hit
TGATTGACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAG10660.1716268323901371No Hit
TGATTGACACTGGGATTAGATACCCCACGATACCTAGAGGAGCCTGTTCT10420.16776281364964618No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACATAAG10300.16583080427940075No Hit
TGATTGACACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTAGG10230.16470379881342426No Hit
TGATTGACACTGGGATTAGATACCCCACCATGCTTAGCCCTAAACCTCAA9640.15520475274305082No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAA9560.1539167464962205No Hit
TGATTGACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAAC9520.15327274337280536No Hit
TGATTGACACTGGGATTAGATACCCCAAGACGATTCCTAGAGGAGCCTGT9440.15198473712597504No Hit
TGATTGACACTGGGATTAGATACCCCTTCCTAGAGGAGCCTGTTCTAGGA9070.14602770823438493No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAG8650.1392656754385259No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATCAA8420.13556265747888877No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAGCCTCAA8040.12944462780644486No Hit
TGATTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC7980.12847862312132213No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAA7540.1213945887637555No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAA7170.11543755987216539No Hit
TGATTGACACTGGGATTAGATACCCCGTCTCGTGGTCTCGTAGAGGAGCC7080.11398855284448128No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGA6890.11092953800825933No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAA6880.11076853722740555No Hit
TGATTGACACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTAGGAA6740.10851452629545254No Hit
TGATTGACACTGGGATTAGATACCCCAGATAAGAGGAGCCTGTTCTAGGA6240.10046448725276318No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTGAC615200.0294.413182
TTGACAC612150.0294.289834
GACACTG610150.0294.076
ACACTGG611150.0293.854287
TGATTGA618650.0293.772641
CACTGGG607450.0293.58038
ATTGACA616050.0293.28873
ACTGGGA608050.0293.12089
TGACACT613150.0293.04015
TACTGGG1100.0281.580028
ACCTGGG1100.0268.171457
CCTGGGA1100.0268.171458
ACACGGG1400.0263.382667
ACGGGAT1400.0263.382669
ACTGGAT4650.0256.925549
CACTGGA5050.0251.178388
ACTGGGT1650.0250.293339
TTGACCT1200.0245.823794
GACATGG1500.0245.823796
CATGGGA1450.0244.128488
Produced by FastQC (version 0.11.5)