FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-22-4-2-6-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-22-4-2-6-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12414
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA254220.476880940873208No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAT136110.963428387304656No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATTCCTCTGT9737.837924923473498No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA5114.11632028355083No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATTCCTC5054.067987755759626No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATTCCTCTG3652.9402287739648783No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAT3302.658289028516191No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC2932.3602384404704364No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA2622.110520380215885No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATTCCT2461.9816336394393428No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA1981.5949734171097147No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT1891.5224746254229098No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA1791.4419204124375706No Hit
GCCACAACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA1551.2485903012727566No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAT1501.208313194780087No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTATTCCTCT1190.9585951345255356No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA1100.8860963428387304No Hit
GCCACAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTATTCCTC1080.8699855002416627No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA930.7491541807636539No Hit
GCCACAACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTATTC810.652489125181247No Hit
GCCACAACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC750.6041565973900435No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA720.5799903334944417No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA680.5477686483003061No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAT630.5074915418076366No Hit
GCCACAACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCT610.4913806992105687No Hit
GCCACAACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTATTCCTC580.467214435314967No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA570.459159014016433No Hit
GCCACAACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTATT520.4188819075237635No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA510.4108264862252296No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA490.3947156436281618No Hit
GCCACAACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTAT490.3947156436281618No Hit
GCCACAACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTATTCCT480.3866602223296278No Hit
GCCACAACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT460.37054937973256No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA430.3463831158369583No Hit
GCCACAACTGGGATTAGATACCCCGACGACTAGAGGAGCCTGTTCTATTC420.3383276945384244No Hit
GCCACAACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTATTCCT410.33027227323989045No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA410.33027227323989045No Hit
GCCACAACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTATTCCTC410.33027227323989045No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA400.3222168519413565No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTATTCCT390.3141614306428226No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCTA360.28999516674722087No Hit
GCCACAACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTATTCCTC340.27388432415015307No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTATT330.26582890285161914No Hit
GCCACAACTGGGATTAGATACCCCACGGGACTAGAGGAGCCTGTTCTATT330.26582890285161914No Hit
GCCACAACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTAT320.2577734815530852No Hit
GCCACAACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTT320.2577734815530852No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC300.2416626389560174No Hit
GCCACAACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTATTCCTC290.2336072176574835No Hit
GCCACAACTGGGATTAGATACCCCGGTAGCTAGAGGAGCCTGTTCTATTC290.2336072176574835No Hit
GCCACAACTGGGATTAGATACCCCGAGCCTAGAGGAGCCTGTTCTATTCC280.2255517963589496No Hit
GCCACAACTGGGATTAGATACCCCTGCTGCAACTAGAGGAGCCTGTTCTA280.2255517963589496No Hit
GCCACAACTGGGATTAGATACCCCTGACTAGAGGAGCCTGTTCTATTCCT270.21749637506041566No Hit
GCCACAACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTATTCCTC270.21749637506041566No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTCT260.20944095376188174No Hit
GCCACAACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTATTCCTC260.20944095376188174No Hit
GCCACAACTGGGATTAGATACCCCTGAGCCCACGAGACCTAGAGGAGCCT250.20138553246334784No Hit
GCCACAACTGGGATTAGATACCCCTGTCAGACTAGAGGAGCCTGTTCTAT250.20138553246334784No Hit
GCCACAACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTATTCCT250.20138553246334784No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA240.1933301111648139No Hit
GCCACAACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTATTCCTC240.1933301111648139No Hit
GCCACAACTGGGATTAGATACCCCAATTAATACGACTCACTAGAGGAGCC230.18527468986628No Hit
GCCACAACTGGGATTAGATACCCCACGATACCTAGAGGAGCCTGTTCTAT230.18527468986628No Hit
GCCACAACTGGGATTAGATACCCCGTCAGACTAGAGGAGCCTGTTCTATT220.17721926856774609No Hit
GCCACAACTGGGATTAGATACCCCACGAGAGCTAGAGGAGCCTGTTCTAT210.1691638472692122No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT210.1691638472692122No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA210.1691638472692122No Hit
GCCACAACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTATTCCTCT200.16110842597067826No Hit
GCCACAACTGGGATTAGATACCCCAGGGAGATCGAGGGCCTAGAGGAGCC200.16110842597067826No Hit
GCCACAACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTATT200.16110842597067826No Hit
GCCACAACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTATT190.15305300467214436No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA180.14499758337361043No Hit
GCCACAACTGGGATTAGATACCCCATGGAACTGCTAGAGGAGCCTGTTCT180.14499758337361043No Hit
GCCACAACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAT180.14499758337361043No Hit
GCCACAACTGGGATTAGATACCCCAGAGGAGCCTGTTCTATTCCTCTGTC170.13694216207507653No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCAGAGACCTAGAGGAGCCTGT170.13694216207507653No Hit
GCCACAACTGGGATTAGATACCCCAATGTCTAGAGGAGCCTGTTCTATTC160.1288867407765426No Hit
GCCACAACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTATTCC160.1288867407765426No Hit
GCCACAACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTATTCCTCT160.1288867407765426No Hit
GCCACAACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCCT160.1288867407765426No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA160.1288867407765426No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG160.1288867407765426No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTC150.1208313194780087No Hit
GCCACAACTGGGATTAGATACCCCTGACGTCTAGAGGAGCCTGTTCTATT150.1208313194780087No Hit
GCCACAACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTATT150.1208313194780087No Hit
GCCACAACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATAT140.1127758981794748No Hit
GCCACAACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTA140.1127758981794748No Hit
GCCACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG140.1127758981794748No Hit
GCCACAACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTATTCC140.1127758981794748No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGATCTAGAGGAGCCTG130.10472047688094087No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGGG12100.0295.06
ACGGGAT108.364502E-4295.07
AACGGGA108.364502E-4295.06
CAACGGG108.364502E-4295.05
CTGGGAT12150.0293.785988
ACTGGGA12150.0293.785987
CAACTGG12250.0292.591835
TGGGATT12150.0292.5729
GCCACAA12500.0291.461
CCACAAC12800.0283.476562
ACAACTG12700.0282.224434
CACAACT12950.0277.915073
CGGGATT150.0028159407196.666678
ACAACGG150.0028159407196.666674
CAGCCCT150.004361015359.00000430-34
GGAGATC303.2908974E-759.00000425-29
TTCATAT2750.059.000004120-124
AACCGCT501.8189894E-1259.00000460-64
CCAGCTT1050.059.00000485-89
AGTAGTC303.2908974E-759.00000425-29
Produced by FastQC (version 0.11.5)