FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-219-6-5-8-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-219-6-5-8-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432215
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA12291828.43908702844649No Hit
TGATTGACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACAGATCT5414512.527330148190135No Hit
TGATTGACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACAGATC183744.251125018798515No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA160413.711347361845378No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGA112162.595004800851428No Hit
TGATTGACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACAGA110892.565621276448064No Hit
TGATTGACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCA104202.410837199079162No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCT89422.068877757597492No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGA85411.9760998577097046No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGA65301.5108221602674594No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGA62401.4437259234408801No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAA47891.1080133729740986No Hit
TGATTGACACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTACAGAT42160.9754404636581331No Hit
TGATTGACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCT41710.965028978633319No Hit
TGATTGACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAA37070.8576749997107921No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAA35390.818805455618153No Hit
TGATTGACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAA33850.7831750401999006No Hit
TGATTGACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAA31570.730423516074176No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAA26910.6226068044838795No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTA26890.62214407181611No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAA22420.5187233205696239No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAA21370.4944298555117245No Hit
TGATTGACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTACAGA19690.4555603114190854No Hit
TGATTGACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTC19320.4469997570653494No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAA19290.44630565806369515No Hit
TGATTGACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACAG19180.44376062839096286No Hit
TGATTGACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCC16760.38776997559085175No Hit
TGATTGACACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCT16570.3833740152470414No Hit
TGATTGACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACAGA15870.36717837187510843No Hit
TGATTGACACTGGGATTAGATACCCTAGAGGAGCCTGTTCTACAGATCTG14850.3435790058188633No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCC14030.3246069664403133No Hit
TGATTGACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTC14010.32414423377254376No Hit
TGATTGACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAG13650.31581504575269254No Hit
TGATTGACACTGGGATTAGATACCCCATTATTCTCTTTTTTAATTTTTTT13290.3074858577328413No Hit
TGATTGACACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAA13220.305866293395648No Hit
TGATTGACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTC12670.2931411450319864No Hit
TGATTGACACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTACA12160.28134146200386384No Hit
TGATTGACACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTACAGA12060.27902779866501626No Hit
TGATTGACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACAG11770.27231817498235833No Hit
TGATTGACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTACAGAT11350.2626007889591986No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTAC10910.25242067026826925No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAA10810.25010700692942167No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGA10320.23877005656906866No Hit
TGATTGACACTGGGATTAGATACCCCATCTGTAGAGGAGCCTGTTCTACA10210.23622502689633632No Hit
TGATTGACACTGGGATTAGATACCCCCACACCTAGAGGAGCCTGTTCTAC9960.2304408685492174No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATA9910.22928403687979362No Hit
TGATTGACACTGGGATTAGATACCCCCCGTTAGAGGAGCCTGTTCTACAG9880.22858993787813936No Hit
TGATTGACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCT9850.22789583887648507No Hit
TGATTGACACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTC9530.2204921161921729No Hit
TGATTGACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAG9410.21771572018555582No Hit
TGATTGACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAA9010.20846106683016555No Hit
TGATTGACACTGGGATTAGATACCCCTCGTCGTCTGCTAGAGGAGCCTGT8980.20776696782851128No Hit
TGATTGACACTGGGATTAGATACCCCGTCTCGTCTAGAGGAGCCTGTTCT8850.20475920548800944No Hit
TGATTGACACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTAC8780.20313964115081612No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACGAGGAGCCTGTTCTACA8670.20059461147808383No Hit
TGATTGACACTGGGATTAGATACCCCGAGACCACGAGACCAGAGGAGCCT8500.19666138380204298No Hit
TGATTGACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTACAGA8250.19087722545492405No Hit
TGATTGACACTGGGATTAGATACCCCTCTCGGCAGCGTCTAGAGGAGCCT8240.1906458591210393No Hit
TGATTGACACTGGGATTAGATACCCCGTCTCGTCGTCTAGAGGAGCCTGT8230.19041449278715455No Hit
TGATTGACACTGGGATTAGATACCCCGAAAAAGAAACGCAAAGTGCCGAA7870.1820853047673033No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGA7580.17537568108464538No Hit
TGATTGACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTACAGA7540.17445021574910635No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAA7310.16912879006975695No Hit
TGATTGACACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTACA7250.16774059206644842No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAG7130.16496419605983134No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATCAA7080.16380736439040755No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAGA7050.1631132653887533No Hit
TGATTGACACTGGGATTAGATACCCCCCCCCTAGAGGAGCCTGTTCTACA7040.16288189905486852No Hit
TGATTGACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC6890.15941140404659718No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGA6760.15640364170609536No Hit
TGATTGACACTGGGATTAGATACCCCCGAGTAGTCTAGAGGAGCCTGTTC6710.15524681003667157No Hit
TGATTGACACTGGGATTAGATACCCCTATAAAGGGCTAGAGGAGCCTGTT6660.1540899783672478No Hit
TGATTGACACTGGGATTAGATACCCCTCGTCGAGGAGCCTGTTCTACAGA6620.15316451303170875No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGA6490.15015675069120693No Hit
TGATTGACACTGGGATTAGATACCCCTCTCGTGGCTAGAGGAGCCTGTTC6410.14830582002012888No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAA6250.14460395867797277No Hit
TGATTGACACTGGGATTAGATACCCCGTCTCGTCTCTCTCTAGAGGAGCC6120.14159619633747092No Hit
TGATTGACACTGGGATTAGATACCCGTCTCGTCTAGAGGAGCCTGTTCTA6070.14043936466804716No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAA5820.13465520632092826No Hit
TGATTGACACTGGGATTAGATACCCCTCTCTCTCTAGAGGAGCCTGTTCT5670.1311847113126569No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCCAAA5550.12840831530603983No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAGCCCAAA5500.12725148363661604No Hit
TGATTGACACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAA5450.12609465196719225No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTGGA5340.12354962229445994No Hit
TGATTGACACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTACA5230.12100459262172761No Hit
TGATTGACACTGGGATTAGATACCCCACTACGCCTAGCCGTAAACTTTGA5210.1205418599539581No Hit
TGATTGACACTGGGATTAGATACCCCGACGAAGAGGAGCCTGTTCTACAG5150.11915366195064955No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAGCCGTAAACCCAGA5070.1173027312795715No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAA4980.1152204342746087No Hit
TGATTGACACTGGGATTAGATACCCCACTATGACTAGCCGTAAACCTTGA4930.11406360260518493No Hit
TGATTGACACTGGGATTAGATACCCCTCTGCTAGAGGAGCCTGTTCTACA4760.11013037492914406No Hit
TGATTGACACTGGGATTAGATACCCCTGTGGGCTAGAGGAGCCTGTTCTA4720.10920490959360504No Hit
TGATTGACACTGGGATTAGATACCCCTGACCTAGAGGAGCCTGTTCTACA4700.10874217692583553No Hit
TGATTGACACTGGGATTAGATACCCCAGTGCAGCTGTTGGAGACTAGAGG4650.10758534525641174No Hit
TGATTGACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTA4630.10712261258864224No Hit
TGATTGACACTGGGATTAGATACCCCTTCGTGGGCTAGAGGAGCCTGTTC4600.10642851358698795No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAGA4550.10527168191756418No Hit
TGATTGACACTGGGATTAGATACCCCCCTATGCCTAGCCGTAAACCTTGA4520.1045775829159099No Hit
TGATTGACACTGGGATTAGATACCCCCCCCGAGGAGCCTGTTCTACAGAT4520.1045775829159099No Hit
TGATTGACACTGGGATTAGATACCCCTCGTCTAGAGGAGCCTGTTCTACA4500.10411485024814039No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAACCCTAA4460.10318938491260138No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTGAC428650.0294.19392
CACTGGG423350.0293.66148
ACACTGG426250.0293.49747
TTGACAC427750.0293.364724
TGATTGA431800.0293.20911
GACACTG426900.0293.119636
ACTGGGA424800.0292.76329
ATTGACA430950.0292.178863
TGACACT429650.0291.621125
TACTGGG600.0270.40328
CATGGGA1350.0240.35848
CCTGGGA500.0235.988258
ACGGGAT650.0226.911779
CACTTGG550.0214.534768
CACTGGA3900.0211.784338
ATGGGAT1600.0202.80249
CAATGGG150.0028495083196.656888
ACTGGGT600.0196.656889
ACTGGAT3800.0194.069299
AACTGGG1450.0193.266227
Produced by FastQC (version 0.11.5)