FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-218-6-5-7-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-218-6-5-7-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342445
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC15826746.216764735943No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT162344.740615281286046No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC115523.3733884273386963No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGGAATCGC115073.360247631006439No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT86532.526829125845026No Hit
CTGGATGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT80992.3650513221101197No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT74772.183416315028691No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT71242.080334068244536No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT71232.080042050548263No Hit
CTGGATGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGGAATCG62211.8166420885105636No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT60611.7699192571069808No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC56881.6609966563973777No Hit
CTGGATGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT51691.509439472032005No Hit
CTGGATGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGGAAT50561.4764414723532246No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT48781.4244623224167383No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAAC36261.058856166683701No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA33070.9657025215728074No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC29380.8579479916482939No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT24660.7201156390077239No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA22630.6608360466644279No Hit
CTGGATGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGGAAT18470.5393566850151119No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAAT15080.44036268597877026No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGAT13660.39889617310809034No Hit
CTGGATGACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGGAATC13420.3918877483975529No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATAT12910.3769948458876608No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAAT12440.36327001416285826No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAAT11790.3442888639051526No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTTAAC11460.33465227992816365No Hit
CTGGATGACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTAGGAATC11410.3331921914468017No Hit
CTGGATGACTGGGATTAGATACCCCCACTAGAGTTACATTAACAAAACCA11310.3302720144840777No Hit
CTGGATGACTGGGATTAGATACCCCTGTAAGAGGAGCCTGTTCTAGGAAT10920.3188833243294544No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGAT10400.30369840412328986No Hit
CTGGATGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA9990.2917256785761217No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAAC9750.2847172538655842No Hit
CTGGATGACTGGGATTAGATACCCCCAGCGATTCCTAGAGGAGCCTGTTC9750.2847172538655842No Hit
CTGGATGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCGAT7720.22543766152228825No Hit
CTGGATGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT6800.198572033465228No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGT6460.18864343179196658No Hit
CTGGATGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG6440.18805939639942182No Hit
CTGGATGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACA6250.18251106017024632No Hit
CTGGATGACTGGGATTAGATACCCCACGATTCCTAGAGGAGCCTGTTCTA6100.1781307947261604No Hit
CTGGATGACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTAGGA5420.15827359137963762No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT5160.15068113127655536No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGAC5070.1480529720101038No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGAC4710.13754033494429763No Hit
CTGGATGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCA4410.1287798040561258No Hit
CTGGATGACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA4230.1235234855232227No Hit
CTGGATGACTGGGATTAGATACCCCGTCTGGTCTAGAGGAGCCTGTTCTA4210.12293945013067793No Hit
CTGGATGACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGT4150.12118734395304355No Hit
CTGGATACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA4020.11739111390150242No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAAC3700.10804654762078582No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAAC3560.10395829987297231No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGGG108.4629626E-4294.99393
CACTGGG108.4629626E-4294.99399
ACGGGAT800.0294.99398
TGCTGGG400.0294.99396
CTGGGGT108.4629626E-4294.99399
TGACTGG336550.0294.906226
GGATGAC339050.0294.776373
CTGGATG340700.0294.73411
TGGATGA340200.0294.603672
GATGACT338350.0294.427154
GACTGGG335100.0294.377667
ATGACTG338300.0293.90395
ACTGGGA335250.0293.761968
CTGGGAT335350.0293.454479
TGACGGG900.0278.605326
CTGGGTT850.0277.64139
CTGGATT1950.0264.73819
GGATGCT450.0262.216773
GACTGGA2200.0261.471837
GCTGGGA400.0258.119667
Produced by FastQC (version 0.11.5)