FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-217-6-1-2-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-217-6-1-2-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323001
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA12789439.59554304785434No Hit
CAACACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATAAGCACTG169905.26004563453364No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA162425.028467404125684No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA104393.2318785390757303No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA83112.5730570493589804No Hit
CAACACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATA61921.9170219287246788No Hit
CAACACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA56041.7349791486713662No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA53541.6575800074922369No Hit
CAACACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT51471.5934935185959176No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA51121.5826576388308395No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA51071.5811096560072568No Hit
CAACACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA42161.3052591168448395No Hit
CAACACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA38211.182968473781815No Hit
CAACACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAT29170.9030931792780827No Hit
CAACACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATAAGCACT29110.9012355998897835No Hit
CAACACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA28860.8934956857718706No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA26380.8167157377221743No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA20840.6451992408692233No Hit
CAACACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTATA20420.6321961851511295No Hit
CAACACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT18990.5879238763966674No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT17510.5421035848186229No Hit
CAACACACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTATAAG17420.5393172157361742No Hit
CAACACACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTATAAGCA15980.49473531041699564No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA15260.47244435775740634No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGATG14980.4637756539453438No Hit
CAACACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTATAAGCA13830.42817204900294425No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA13570.4201225383203148No Hit
CAACACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTATAAGCAC13470.41702657267314963No Hit
CAACACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATAAGC12890.3990699719195916No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATA12310.3811133711660335No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATA12240.3789461952130179No Hit
CAACACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATAAGCA11210.3470577490472166No Hit
CAACACACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTATAA10500.3250763929523438No Hit
CAACACGCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA10060.311454144104817No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAACA9810.30371422998690406No Hit
CAACACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG9020.2792561013742991No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATCGATT8690.26903941473865406No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATA8300.2569651487147099No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGACA8290.25665555214999336No Hit
CAACACACTGGGATTAGATACCCCGTCTCGTCTAGAGGAGCCTGTTCTAT7980.24705805864378128No Hit
CAACACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATA7850.24303330330246656No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA7830.24241411017303355No Hit
CAACACACTGGGATTAGATACCCCCCCGTCCTAGAGGAGCCTGTTCTATA7770.2405565307847344No Hit
CAACACACTGGGATTAGATACCCCTGGCGAGAGACTGTCTCTAGAGGAGC7460.23095903727852235No Hit
CAACACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTA7270.22507670254890852No Hit
CAACACACTGGGATTAGATACCCCACGAGACGTCTAGAGGAGCCTGTTCT6890.21331203308968083No Hit
CAACACACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTATAAGCAC6840.21176405026609824No Hit
CAACACACTGGGATTAGACACCCCACTATGCTTAGCCCTAAACCTCAACA6390.19783220485385494No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAACA6330.19597462546555583No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAT6280.19442664264197324No Hit
CAACACACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAATA6250.19349785294782368No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACA5970.18482914913576118No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAGCCCTAAACCTCAACA5960.1845195525710447No Hit
CAACACACTGGGATTAGATACCCCTCTATACTAAATTACTTTATCCTATT5950.18420995600632817No Hit
CAACACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAGCTCTAATA5390.16687254838220314No Hit
CAACACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG5220.16160940678202235No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTTGGTA5210.16129981021730583No Hit
CAACACACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTATAAGCA5100.15789424800542412No Hit
CAACACACTGGGATTAGATACCCCGCACTAGAGGAGCCTGTTCTATAAGC4980.15417908922882592No Hit
CAACACACTGGGATTAGATACCCCTCGTCGGCAGCGTCAGATGTGTATAG4520.13993764725186608No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAGATA4290.13281692626338618No Hit
CAACACACTGGGATTAGATACCCCATTATTCTCTTTTTTAATTTTTTTTT4280.13250732969866966No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA4150.12848257435735494No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAATA4100.12693459153377235No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACA4020.12445781901604021No Hit
CAACACACTGGGATTAGATACCCCTGACGCTAAGAGGAGCCTGTTCTATA4000.12383862588660716No Hit
CAACACACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATA3960.1226002396277411No Hit
CAACACCCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA3780.11702750146284377No Hit
CAACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG3750.1160987117686942No Hit
CAACACACTGGGATTAGATACCCCGTCTCTCTAGAGGAGCCTGTTCTATA3710.11486032550982815No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGTA3690.11424113238039511No Hit
CAACACACTGGGATTAGATACCCCACGAGAGGAGCCTGTTCTATAAGCAC3620.11207395642737947No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAGATA3570.1105259736037969No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAATA3450.10681081482719869No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA3280.10154767322701787No Hit
CAACACACTGGGATTAGATACCCCCGTCTAGAGGAGCCTGTTCTATAAGC3270.10123807666230136No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGGAT157.236189E-6294.99358
CTTGGAT206.178743E-8294.993478
CACTGGG310500.0294.518466
ACACTGG313700.0294.241185
ACTGGGA310950.0293.47567
CACACTG315650.0293.124364
CTGGGAT315350.0293.075848
TGGGATT315100.0292.278569
CGCTGGG2850.0289.818186
TGGGATA1500.0285.160379
GCTGGGA2900.0284.821327
CCCTGGG1250.0283.193766
ACGCTGG2950.0279.993845
ACACACT332800.0278.417853
ACCCTGG1300.0272.30175
CACGCTG3050.0270.81374
CTGGGTT600.0270.41078
ACTGGAT2900.0269.563027
CTGGATT2850.0269.116888
ACACGGG950.0263.941565
Produced by FastQC (version 0.11.5)