FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-216-6-1-2-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-216-6-1-2-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252110
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA9313736.94300107096109No Hit
CAACACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGCTGAGAAC180057.141723850700091No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA113164.488516917218675No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA64002.5385744317956447No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA61532.4406013248185316No Hit
CAACACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT48851.9376462655190194No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA41191.6338106382134783No Hit
CAACACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATA39311.5592400142794811No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA37231.4767363452461226No Hit
CAACACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA34341.3621038435603507No Hit
CAACACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA33091.312522311689342No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA28751.1403752330331998No Hit
CAACACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGCTGAGA28021.1114196184205307No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA24310.964261631827377No Hit
CAACACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG24290.9634683273174408No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA22300.884534528578795No Hit
CAACACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA22150.8785847447542741No Hit
CAACACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGCTGAGAA20970.8317797786680416No Hit
CAACACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA20110.7976676847407876No Hit
CAACACACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTAGCTG19870.788148030621554No Hit
CAACACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAGC17930.7111974931577486No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGATG17530.6953314029590258No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA15680.621950735789933No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA13320.5283408036174686No Hit
CAACACACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTAGCTGAG12440.49343540518027845No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATA12130.4811391852762683No Hit
CAACACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGCTGA10170.40339534330252663No Hit
CAACACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGCTGAG9930.393875689183293No Hit
CAACACACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTAGCT9860.39109912339851655No Hit
CAACACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGCTGAG9860.39109912339851655No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT9700.3847526873190274No Hit
CAACACACTGGGATTAGATACCCCACGAGACGTCTAGAGGAGCCTGTTCT9310.36928324937527274No Hit
CAACACACTGGGATTAGATACCCCGCACTAGAGGAGCCTGTTCTAGCTGA8160.3236682400539447No Hit
CAACACACTGGGATTAGATACCCCCCCGTCCTAGAGGAGCCTGTTCTAGC8070.32009836975923206No Hit
CAACACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT8020.31811510848439173No Hit
CAACACGCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA7650.30343897505057316No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGTA7010.27805323073261673No Hit
CAACACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG6830.27091349014319144No Hit
CAACACACTGGGATTAGATACCCCACGAGAGGAGCCTGTTCTAGCTGAGA6810.27012018563325535No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAG6400.2538574431795645No Hit
CAACACACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTAGCTGAG6190.245527745825235No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATA6050.23997461425568203No Hit
CAACACACTGGGATTAGATACCCCGTCTCGTCTAGAGGAGCCTGTTCTAG6030.23918130974574592No Hit
CAACACACTGGGATTAGATACCCCGTCTCTCTAGAGGAGCCTGTTCTAGC5680.2252984808218635No Hit
CAACACACTGGGATTAGATACCCCTCTATACTAAATTACTTTATCCTATT5320.21101899964301296No Hit
CAACACACTGGGATTAGACACCCCACTATGCTTAGCCCTAAACCTCAACA5230.20744912934830034No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAACA5170.20506921581849194No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACA4970.19713617071913056No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA4960.19673951846416246No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA4940.19594621395422632No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTTGGTA4930.19554956169925827No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAATA4920.1951529094442902No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATCGATT4840.19197969140454563No Hit
CAACACACTGGGATTAGATACCCCCGAGAGGAGCCTGTTCTAGCTGAGAA4420.1753202966958867No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACA4400.17452699218595058No Hit
CAACACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGCTGAG4340.17214707865614215No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATA4200.16659394708658917No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA4200.16659394708658917No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAGATA4140.16421403355678077No Hit
CAACACACTGGGATTAGATACCCCTGACGCTAAGAGGAGCCTGTTCTAGC4080.16183412002697234No Hit
CAACACACTGGGATTAGATACCCCGTCTCGTCGTCGTCGTCGTCTAGAGG4060.1610408155170362No Hit
CAACACACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATA3910.15509103169251517No Hit
CAACACACTGGGATTAGATACCCCATTATGCCCCGCCGTAAACTTAGATA3880.15390107492761096No Hit
CAACACACTGGGATTAGATACCCCACCATGCTTAGCCCTAAACCTCAACA3840.15231446590773867No Hit
CAACACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG3710.1471579865931538No Hit
CAACACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTA3570.14160485502360082No Hit
CAACACACTGGGATTAGATACCCCCTATGCTTAGCCCTAAACTCTAATAG3560.14120820276863275No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA3520.13962159374876046No Hit
CAACACACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTAGCTGAGA3490.13843163698385624No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAACA3320.13168854864939908No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCGTAAACCCAGACG3300.13089524413946294No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAACA3270.12970528737455872No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAACA3110.12335885129506961No Hit
CAACACACTGGGATTAGATACCCCTGGCGAGAGACTGTCTCTAGAGGAGC2930.11621911070564438No Hit
CAACACCCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA2860.11344254492086789No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAGCCCTAAACCTCAACA2790.1106659791360914No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGACA2760.10947602237118718No Hit
CAACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2620.10392289080163421No Hit
CAACACACTGGGATTAGATACCCCAATACGCTTAGCCCTAAACTCCAATA2580.10233628178176193No Hit
CAACACACTGGGATTAGATACCCCTCGTCGGCAGCGTCAGATGTGTATAG2580.10233628178176193No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGG2150.0294.98956
CAGTGGG400.0294.98956
CTGGGTT500.0294.98958
CATGGGA350.0294.989476
CTGGGAG157.234703E-6294.989478
CACTGGG243350.0294.92896
ACACTGG245650.0294.268985
ACTGGGA243500.0294.141487
CTGGGAT246550.0293.613568
TGGGATT246650.0293.25539
CACACTG247600.0292.547124
ACACACT259800.0279.263523
CTGGATT1750.0278.132938
CCCTGGG800.0276.552646
ACGCTGG2300.0275.751045
ACTGGGT600.0270.4077
GCTGGGA2350.0269.8847
CACCTGG450.0262.212864
TGGATTA1800.0262.212869
ACCCTGG850.0260.284855
Produced by FastQC (version 0.11.5)