FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-215-6-1-2-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-215-6-1-2-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215121
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA8008937.22974512018817No Hit
CAACACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAACTTCGCCT130766.078439575866605No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA90784.219950632434769No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA56212.612948061788482No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA51122.376337038224999No Hit
CAACACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT42011.9528544400593155No Hit
CAACACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATA41151.9128769390250138No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA38721.799917255869952No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA36991.7194973991381595No Hit
CAACACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA34271.5930569307506008No Hit
CAACACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA30681.4261741066655511No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA25881.2030438683345652No Hit
CAACACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA25791.1988601763658593No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA24261.1277374128978574No Hit
CAACACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAA21841.0152425844059854No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA19940.9269201983999702No Hit
CAACACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA19830.9218067971048852No Hit
CAACACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAACTTCGC19780.9194825237889374No Hit
CAACACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAAC18140.8432463590258507No Hit
CAACACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAACTTCGCC16950.7879286541062936No Hit
CAACACACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTAACTT16880.7846746714639669No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGATG16030.7451620250928547No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA13040.6061704807991781No Hit
CAACACACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTAACTTCG12600.5857168756188378No Hit
CAACACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT10660.4955350709600644No Hit
CAACACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAACTTC10370.4820542857275673No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA10370.4820542857275673No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATA8850.41139637692275505No Hit
CAACACACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTAACT8730.40581812096448044No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT7510.3491058520553549No Hit
CAACACGCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA6760.3142417523161384No Hit
CAACACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAACTTCG6740.31331204298975923No Hit
CAACACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTA6700.311452624337001No Hit
CAACACACTGGGATTAGATACCCCGCACTAGAGGAGCCTGTTCTAACTTC6500.30215553107320997No Hit
CAACACACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTAACTTCGC6450.2998312577572622No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATA6380.2965772751149353No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCGTAAACCCAGACG6250.29053416449347114No Hit
CAACACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAACTTCG6090.28309648988243824No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA6000.27891279791373225No Hit
CAACACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG5880.27333454195545764No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGTA5860.27240483262907855No Hit
CAACACACTGGGATTAGATACCCCACGAGAGGAGCCTGTTCTAACTTCGC5850.2719399779658889No Hit
CAACACACTGGGATTAGATACCCCCCCGTCCTAGAGGAGCCTGTTCTAAC5240.2435838435113262No Hit
CAACACACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTAACTTCG5230.24311898884813665No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAACA5100.23707587822667242No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAACA5030.23382189558434555No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAA4960.2305679129420187No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAATA4890.2273139302996918No Hit
CAACACACTGGGATTAGATACCCCACGAGACGTCTAGAGGAGCCTGTTCT4500.2091845984352992No Hit
CAACACACTGGGATTAGACACCCCACTATGCTTAGCCCTAAACCTCAACA4340.20174692382426634No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA4300.19988750517150813No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATA3920.1822230279703051No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTTGGTA3870.1798987546543573No Hit
CAACACACTGGGATTAGATACCCCTCTATACTAAATTACTTTATCCTATT3790.1761799173488409No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGACA3710.17246108004332444No Hit
CAACACACTGGGATTAGATACCCCGTCTCGTCTAGAGGAGCCTGTTCTAA3680.1710665160537558No Hit
CAACACACTGGGATTAGATACCCCGTCTCTCTAGAGGAGCCTGTTCTAAC3580.16641796942186024No Hit
CAACACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG3510.16316398677953337No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACA3170.14735892823108857No Hit
CAACACACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATA3160.146894073567899No Hit
CAACACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATA3140.14596436424151987No Hit
CAACACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAACTTCG2910.13527270698816016No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATCGATT2870.13341328833540192No Hit
CAACACACTGGGATTAGATACCCCTGGCGAGAGACTGTCTCTAGAGGAGC2600.12086221242928398No Hit
CAACACACTGGGATTAGATACCCCCGTCTAGAGGAGCCTGTTCTAACTTC2590.12039735776609443No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA2590.12039735776609443No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAGACCTCAACA2540.11807308445014666No Hit
CAACACACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAATA2520.11714337512376755No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAGCCCTAAACCTCAACA2460.11435424714463023No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACA2410.11202997382868246No Hit
CAACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2400.11156511916549292No Hit
CAACACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAGCTCTAATA2370.11017055517592424No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAGATA2330.10831113652316604No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA2320.1078462818599765No Hit
CAACACCCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA2320.1078462818599765No Hit
CAACACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAACTTC2250.1045922992176496No Hit
CAACACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGC2250.1045922992176496No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAACA2210.10273288056489138No Hit
CAACACACTGGGATTAGATACCCCACCATGCTTAGCCCTAAACCTCAACA2200.10226802590170184No Hit
CAACACACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2200.10226802590170184No Hit
CAACACACTGGGATTAGATACCCCATTATTCTCTTTTTTAATTTTTTTTT2160.10040860724894363No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGA550.0294.992346
ACGCTGG2050.0294.992345
ACATGGG550.0294.992345
ACCGGGA108.460912E-4294.99237
GCTGGGA2000.0294.99237
TGGGACT108.460912E-4294.99239
CACTGGG206750.0294.706976
ACACTGG209000.0293.863165
ACTGGGA207350.0293.498547
CTGGGAT210450.0292.53938
CACACTG210750.0292.05294
TGGGATT210250.0291.835459
CACCTGG800.0276.55534
ACACACT223250.0276.0973
ACACGGG650.0272.30065
CACATGG600.0270.409644
ACGGGAT600.0270.409647
CCCTGGG600.0270.409646
CGCTGGG2300.0262.927956
AACTGGG900.0262.215425
Produced by FastQC (version 0.11.5)