FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-214-6-1-1-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-214-6-1-1-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196731
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG7919440.25496744285344No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG113285.758116412766672No Hit
GGTACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACAGATCTGTC79584.045117444632519No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG58902.9939358819911455No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC54002.7448648154078414No Hit
GGTACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATAT44422.257905464822524No Hit
GGTACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT37631.9127641296999458No Hit
GGTACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG36001.8299098769385607No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG35221.790261829604892No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG33161.6855503199800743No Hit
GGTACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT28291.438004178294219No Hit
GGTACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG27781.4120804550375894No Hit
GGTACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG25061.273820597668898No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG21071.0710055863082075No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG16900.8590410255628244No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG16350.8310840691095963No Hit
GGTACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTACA16170.8219345197249036No Hit
GGTACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTACAGATCTG15980.8122766620410612No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGATGC15240.7746618479039907No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG14130.7182396266983851No Hit
GGTACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTACAG13070.6643589469885275No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG12230.6216610498599611No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATTA11370.5779465361330954No Hit
GGTACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT10620.5398234136968754No Hit
GGTACACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTACAGAT9240.46967686841423056No Hit
GGTACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACAGATCTGT9100.46256055222613623No Hit
GGTACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTACAGATCT8580.43612852067035696No Hit
GGTACACTGGGATTAGATACCCCTATAAGAGACAGTCTCAATCTAGAGGA7270.36954013348175935No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG7180.364965358789413No Hit
GGTACACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTACAGATCT6870.34920780151577535No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGACAG6640.33751671063533456No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATAG6370.32379238655829534No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATAG6230.3166760703702009No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATAC6190.31464283717360253No Hit
GGTACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTAG5990.3044766711906105No Hit
GGTACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT5730.29126065541272095No Hit
GGTACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACAGATC5720.29075234711357134No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAATAG5710.29024403881442173No Hit
GGTACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAGCTCTAATAG5390.27397817324163454No Hit
GGTACACTGGGATTAGATACCCCCCCGTCCTAGAGGAGCCTGTTCTACAG4870.2475461416858553No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTACA4340.2206058018309265No Hit
GGTACACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTACAGA4300.21857256863432808No Hit
GGTACACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAATAG4270.2170476437368793No Hit
GGTACACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTACAGATCTG4030.2048482445572889No Hit
GGTACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT3930.19976516156579288No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATCGATTG3870.1967153117708953No Hit
GGTACACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATAA3670.18654914578790327No Hit
GGTACACTGGGATTAGATACCCCACGAGACGTCTAGAGGAGCCTGTTCTA3550.18044944619810807No Hit
GGTACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACAGATCT3530.17943282959980889No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGTAG3460.17587467150576166No Hit
GGTACACTGGGATTAGATACCCCGCACTAGAGGAGCCTGTTCTACAGATC3400.17282482171086408No Hit
GGTACACTGGGATTAGATACCCCTCTATACTAAATTACTTTATCCTATTA3300.16774173871936807No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG3300.16774173871936807No Hit
GGTACACTGGGATTAGATACCCCATTATTCTCTTTTTTAATTTTTTTTTA3030.15401741464232888No Hit
GGTACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATAA3020.15350910634317927No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACAG3000.15249248974488006No Hit
GGTACACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTACAGATCT2990.15198418144573045No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAGATAG2900.14740940675338407No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCGTAAACCCAGACGT2870.14588448185593525No Hit
GGTACACTGGGATTAGATACCCCTCGTCGGCAGCGTCAGATGTGTATAGA2840.14435955695848646No Hit
GGTACACTGGGATTAGATACCCCGTCTCTCTAGAGGAGCCTGTTCTACAG2820.14334294036018727No Hit
GGTACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACAGATCT2580.13114354118059685No Hit
GGTACACTGGGATTAGATACCCCACCATGCTTAGCCCTAAACCTCAACAG2560.13012692458229766No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAGACCTCAACAG2520.12809369138569926No Hit
GGTACACTGGGATTAGATACCCCCTATGCTTAGCCCTAAACTCTAATAGT2400.12199399179590406No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAACAG2370.12046906689845524No Hit
GGTACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAC2310.11741921710355764No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAACAG2250.11436936730866004No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTTGGTAA2210.11233613411206166No Hit
GGTACACTGGGATTAGATACCCCGTCTCGTCTAGAGGAGCCTGTTCTACA2170.11030290091546326No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAACAG2120.10776135941971525No Hit
GGTACACTGGGATTAGATACCCCATTATGCCCCGCCGTAAACTTAGATAT2070.10521981792396724No Hit
GGTACACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT2020.10267827642821924No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGC157.234097E-6294.971041
GCTGGGA157.234097E-6294.971046
CGCTGGG157.234097E-6294.971045
GGGTTAG305.456968E-12294.971049
ACCGGGA255.293259E-10294.9716
CCGGGAT255.293259E-10294.9717
TGGAATT206.174923E-8294.9718
CTGGTTA108.462193E-4294.9717
CTGGGTT350.0294.9717
TGGGATG108.462193E-4294.9718
TGGGATC108.462193E-4294.9718
CTAGGAT255.293259E-10294.9717
GGGATAG850.0294.9719
ACTGGGT400.0294.9716
ACTGGGA191300.0294.893926
CACTGGG191850.0294.817265
GGTACAC195800.0294.669741
CTGGGAT191750.0294.43267
ACACTGG193950.0294.362674
GTACACT195000.0293.912142
Produced by FastQC (version 0.11.5)