FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-213-6-5-8-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-213-6-5-8-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189034
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA7069437.39750521070284No Hit
TGATTGACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGATTGAG186089.843731815440607No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA51882.744479829025466No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGA40472.140884708570945No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGA36081.9086513537247267No Hit
TGATTGACACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTAGA32031.6944041812583979No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAA31991.6922881598019406No Hit
TGATTGACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGATTG31991.6922881598019406No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAA30031.5886031084355194No Hit
TGATTGACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAA26361.3944581398055376No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAA25911.37065289842039No Hit
TGATTGACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCA22481.1892040585291535No Hit
TGATTGACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAA21711.1484706454923452No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGA21461.1352455113894855No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGA20591.0892220447115333No Hit
TGATTGACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGATTGA19061.0082842240020313No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTA18931.0014071542685443No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCT17580.9299914301131014No Hit
TGATTGACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAA16470.8712718346964039No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAA15350.8120232339155918No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAA14800.7829279388893002No Hit
TGATTGACACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTAGATT14270.7548906545912375No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGA14080.7448395526730641No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTAG13550.7168022683750013No Hit
TGATTGACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGAT11740.6210522974702963No Hit
TGATTGACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTC11000.5819059005258314No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAA10620.5618036966894845No Hit
TGATTGACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCC7940.42003025910682734No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGA7800.4126241840092259No Hit
TGATTGACACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTAGATTG7090.375064803157104No Hit
TGATTGACACTGGGATTAGATACCCCGTCTCTCTAGAGGAGCCTGTTCTA6810.36025265296190107No Hit
TGATTGACACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTAGATT6680.3533755832284139No Hit
TGATTGACACTGGGATTAGATACCCCCCCGTCCTAGAGGAGCCTGTTCTA6400.33856343303321096No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATA6300.333273379392067No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACGTCTAGAGGAGCCTGTT6220.3290413364791519No Hit
TGATTGACACTGGGATTAGATACCCCACGAGAGGAGCCTGTTCTAGATTG6170.3263963096585799No Hit
TGATTGACACTGGGATTAGATACCCCGTCTCGTCTAGAGGAGCCTGTTCT6130.3242802882021224No Hit
TGATTGACACTGGGATTAGATACCCCGCACTAGAGGAGCCTGTTCTAGAT5750.3041780843657755No Hit
TGATTGACACTGGGATTAGATACCCCTCTATACTAAATTACTTTATCCTA5710.3020620629093179No Hit
TGATTGACACTGGGATTAGATACCCCCGAGAGGAGCCTGTTCTAGATTGA5250.2777278161600559No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAA5070.2682057196059968No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGA4980.2634446713289673No Hit
TGATTGACACTGGGATTAGATACCCCTGACGCTAAGAGGAGCCTGTTCTA4930.2607996445083953No Hit
TGATTGACACTGGGATTAGATACCCTCTAGAGGAGCCTGTTCTAGATTGA4890.25868362305193776No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAGCCGTAAACCCAGA4770.252335558682565No Hit
TGATTGACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAG4760.25180655331845064No Hit
TGATTGACACTGGGATTAGATACCCCTCGTCGGCAGCGTCAGATGTGTAT4650.2459874943131923No Hit
TGATTGACACTGGGATTAGATACCCGTCTCGTCTCTAGAGGAGCCTGTTC4450.23540738703090447No Hit
TGATTGACACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAA4060.2147761778304432No Hit
TGATTGACACTGGGATTAGATACCCCATTATTCTCTTTTTTAATTTTTTT4020.2126601563739856No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAGA3920.2073701027328417No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATCGA3890.20578308664049852No Hit
TGATTGACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC3860.20419607054815536No Hit
TGATTGACACTGGGATTAGATACCCCGTCTCGTAGAGGAGCCTGTTCTAG3840.20313805981992658No Hit
TGATTGACACTGGGATTAGATACCCCGACGAGGAGCCTGTTCTAGATTGA3770.19943502227112586No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAA3560.1883259096247236No Hit
TGATTGACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAA3420.1809198345271221No Hit
TGATTGACACTGGGATTAGATACCCCTGGCGAGAGACTGTCTCTAGAGGA3360.17774580234243575No Hit
TGATTGACACTGGGATTAGATACCCCGTCTCGTCGTCGTCGTCGTCTAGA3360.17774580234243575No Hit
TGATTGACACTGGGATTAGATACCCTCGTCTAGAGGAGCCTGTTCTAGAT3290.174042764793635No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAA3280.17351375942952063No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAA3180.1682237057883767No Hit
TGATTGACACTGGGATTAGATACCCCAGAGACAGAGGAGCCTGTTCTAGA2990.15817260387020324No Hit
TGATTGACACTGGGATTAGATACCCCAGAGGAGCCTGTTCTAGATTGAGA2880.15235354486494493No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAA2850.15076652877260174No Hit
TGATTGACACTGGGATTAGATACCCCAATACGCTTAGCCCTAAACTCCAA2680.1417734375826571No Hit
TGATTGACACTGGGATTAGATACCCTCGTCGGCAGCGTCAGATGTGTAGA2660.1407154268544283No Hit
TGATTGACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAGCTCTAA2650.1401864214903139No Hit
TGATTGACACTGGGATTAGATACCCCAGTCTAGAGGAGCCTGTTCTAGAT2490.13172233566448366No Hit
TGATTGACACTGGGATTAGATACCCCTATAAGAGACAGTCTCAATCTAGA2480.13119333030036923No Hit
TGATTGACACTGGGATTAGATACCCCGTCTCGTGGTCTAGAGGAGCCTGT2390.1264322820233397No Hit
TGATTGACACTGGGATTAGATACCCCGAGGAGCCTGTTCTAGATTGAGAC2350.12431626056688214No Hit
TGATTGACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAAC2340.12378725520276776No Hit
TGATTGACACTGGGATTAGATACCCCCTCGTCGGCAGCGTCAGATGTGTA2320.12272924447453896No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAA2240.11849720156162384No Hit
TGATTGACACTGGGATTAGATACCCCTCCGAGCCCACGAGACTAGAGGAG2240.11849720156162384No Hit
TGATTGACACTGGGATTAGATACCCAACTATGCTTAGCCCTAAACTATAA2100.11109112646402233No Hit
TGATTGACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGA2090.11056212109990796No Hit
TGATTGACACTGGGATTAGATACCCCCTCGTGGGAGGAGCCTGTTCTAGA2090.11056212109990796No Hit
TGATTGACACTGGGATTAGATACCCGTCTCGTCTAGAGGAGCCTGTTCTA2090.11056212109990796No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAA1990.10527206745876404No Hit
TGATTGACACTGGGATTAGATACCCTAGAGGAGCCTGTTCTAGATTGAGA1950.10315604600230648No Hit
TGATTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC1920.10156902990996329No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAA1900.1005110191817345No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGA108.4594416E-4295.09
CACCGGG108.4594416E-4295.08
ATTGACC255.293259E-10295.03
CACTTGG157.230814E-6294.999978
GATTGAC187450.0294.29182
CACTGGG184150.0293.878638
TTGACAC186900.0293.816224
ACTGGGA184700.0293.482679
TGACACT186400.0293.259125
ACACTGG185900.0293.254437
GACACTG186000.0293.17616
ATTGACA188000.0292.80323
TGATTGA189300.0292.50661
TACTGGG450.0262.222238
ACACGGG650.0249.615397
CCTGGGA301.5770638E-9245.833318
AACTGGG251.8780338E-7236.07
GACATGG500.0236.06
CACTGGA1450.0233.965528
ACGGGAT600.0221.249989
Produced by FastQC (version 0.11.5)