FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-212-6-1-1-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-212-6-1-1-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185931
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG7516740.4273628389026No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG102465.51064642259763No Hit
GGTACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATAAGCACTGT86234.637741958038196No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC54632.938186746696355No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG50852.7348855220485015No Hit
GGTACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT42642.293323867456207No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG35591.9141509484701316No Hit
GGTACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATAT33851.8205678450608023No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG29061.5629453937213267No Hit
GGTACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG27671.4881864777793912No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG25301.3607198369287532No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG24961.342433483388999No Hit
GGTACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG20211.086962367760083No Hit
GGTACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG19731.0611463392333715No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG18590.9998332714824316No Hit
GGTACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT18500.9949927661336733No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGATGC18270.9826225857979574No Hit
GGTACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATAAGCACTG14500.7798591950777439No Hit
GGTACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTATA14010.7535053326233925No Hit
GGTACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTATAAGCACT11090.5964578257525641No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG10930.5878524829103269No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATTA9890.5319177544357854No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG9560.5141692348236712No Hit
GGTACACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTATAAGC9460.5087908955472729No Hit
GGTACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTATAA9160.4926558777180782No Hit
GGTACACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTATAAGCAC7570.4071402832233463No Hit
GGTACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTATAAGCAC7340.39477010288763037No Hit
GGTACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT6980.37540808149259675No Hit
GGTACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATAAGCA6620.35604606009756307No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATAG6580.35389472438700376No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAATAG6400.34421371368948694No Hit
GGTACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATAAGCAC6190.33291920120905066No Hit
GGTACACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTATAAG5770.3103301762481781No Hit
GGTACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT5340.2872033173596657No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATAC5330.28666548343202586No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATAG5050.2716061334581108No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCGTAAACCCAGACGT5010.26945479774755154No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGTAG4890.2630007906158736No Hit
GGTACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTAG4840.2603116209776745No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG4290.23073075495748424No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTATA4280.2301929210298444No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAGATAG4220.2269659174640055No Hit
GGTACACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATAA4100.2205119103323276No Hit
GGTACACTGGGATTAGATACCCCTCGTCGGCAGCGTCAGATGTGTATAGA4080.21943624247704793No Hit
GGTACACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTATAAGCACT3890.20921739785189128No Hit
GGTACACTGGGATTAGATACCCCTCTATACTAAATTACTTTATCCTATTA3600.1936202139503364No Hit
GGTACACTGGGATTAGATACCCCTGACGCTAAGAGGAGCCTGTTCTATAA3500.1882418746739382No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACAG3480.18716620681865853No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTTGGTAA3410.18340136932517978No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAACAG3290.17694736219350188No Hit
GGTACACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTATAAGCAC3210.1726446907723833No Hit
GGTACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT3190.17156902291710366No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGACAG3100.16672851756834525No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAACAG3060.16457718185778594No Hit
GGTACACTGGGATTAGATACCCCATTATGCCCCGCCGTAAACTTAGATAT2990.1608123443643072No Hit
GGTACACTGGGATTAGATACCCCATTATTCTCTTTTTTAATTTTTTTTTA2930.15758534079846823No Hit
GGTACACTGGGATTAGATACCCCACGAGACGTCTAGAGGAGCCTGTTCTA2910.1565096729431886No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGATGA2870.15435833723262932No Hit
GGTACACTGGGATTAGATACCCCCCCGTCCTAGAGGAGCCTGTTCTATAA2650.1425259908245532No Hit
GGTACACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGTTC2600.13983682118635407No Hit
GGTACACTGGGATTAGATACCCCACGAGAGGAGCCTGTTCTATAAGCACT2600.13983682118635407No Hit
GGTACACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAATAG2540.13660981762051513No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAT2500.13445848190995585No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG2270.1220883015742399No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAACAG2260.12155046764660009No Hit
GGTACACTGGGATTAGATACCCCGTCTCGTCTAGAGGAGCCTGTTCTATA2240.12047479979132042No Hit
GGTACACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT2210.11886129800840098No Hit
GGTACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTATAAGCAC2160.11617212837020185No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAGCCCTAAACCTCAACAG2140.1150964605149222No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAACAG2120.11402079265964256No Hit
GGTACACTGGGATTAGATACCCCGTCTCTCTAGAGGAGCCTGTTCTATAA2040.10971812123852397No Hit
GGTACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAT1990.10702895160032484No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAT1940.10433978196212573No Hit
GGTACACTGGGATTAGATACCCCACCATGCTTAGCCCTAAACCTCAACAG1930.10380194803448592No Hit
GGTACACTGGGATTAGATACCCCGAGTACTCCGGCTTGAGGAATGCGTCT1860.10003711054100714No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGGG500.0294.993044
ATGGGAT600.0294.9936
TTGGATT157.231307E-6294.9938
ACTTGGA157.231307E-6294.9936
ACTCTGG108.459931E-4294.9934
CTTGGAT157.231307E-6294.9937
CTGGGTT350.0294.9937
TCTGGGA108.459931E-4294.9936
GGGATTG400.0294.9939
ACTGGGT350.0294.9936
CACTGGG182200.0294.66925
ACTGGGA181900.0294.58766
GGTACAC184950.0294.514531
CTGGGAT181900.0294.50657
ACACTGG183900.0294.110754
TGGGATT181700.0293.531868
GTACACT184900.0293.477362
TACACTG184600.0293.47493
GGGATTA181900.0292.64159
GGGATAG800.0276.555949
Produced by FastQC (version 0.11.5)