FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-211-6-4-6-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-211-6-4-6-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178254
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA7154340.13542473100183No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA111776.270266024885837No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGCTGAGAAC57733.238637001133214No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG54603.0630448685583493No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA41352.3197235405657093No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA35121.9702222671020004No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT34731.9483433751837267No Hit
GCCACAACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATA32501.8232409931894937No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA30731.7239444837142504No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA29321.64484387447126No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATA29171.636428916041155No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTTATG26101.4642027668383317No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA24501.3744432102505413No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA23511.318904484611846No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGGTG23131.2975865899222458No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA21371.1988510776756762No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGCTGAG19311.0832856485688962No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA17200.9649152333187474No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA14840.8325198873517564No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA13140.7371503584772291No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT12740.7147104693302815No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCCAAACCTCAACA9640.5408013284414375No Hit
GCCACAACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAGC9080.5093854836357109No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGCTGAGAA7950.44599279679558385No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCATAAACATTGACA7080.3971860379009728No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGATG6740.3781121321260673No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA6610.37081916815330934No Hit
GCCACAACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATA6540.3668921875525935No Hit
GCCACAACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAATA6440.3612822152658566No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA5650.31696343420063505No Hit
GCCACAACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTAGCTG5260.29508454228236114No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAGATA5140.28835257553827687No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGCTGA4830.27096166144939243No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACATTGGTA4570.2563757335038765No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAACA4500.25244875290316066No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG4340.24347279724438162No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA4280.24010681387233948No Hit
GCCACAACTGGGATTAGATACCCCAATACGCTTAGCCCTAAACTCCAATA4240.23786282495764471No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA4190.23505783881427625No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATA4160.23337484712825518No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAGCCCTAAACCTCAACA4050.22720387761284458No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGATG4030.2260818831554972No Hit
GCCACAACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT3830.21486193858202338No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATTGGCA3730.2092519662952865No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAGTCTTGATA3730.2092519662952865No Hit
GCCACAACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTA3710.2081299718379391No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGCTGA3560.19971501340783374No Hit
GCCACAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGCTGAG3480.19522703557844423No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAG3430.1924220494350758No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT3280.18400709100497042No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGCTGAGA3200.17951911317558092No Hit
GCCACAACTGGGATTAGATACCCCCACCAAGACCGGGTAAACTTAACATT3170.17783612148955985No Hit
GCCACAACTGGGATTAGATACCCCATTATTCTCTTTTTTAATTTTTTTTT3010.16886016583078078No Hit
GCCACAACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTAGCTGAG3010.16886016583078078No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATA3000.1682991686021071No Hit
GCCACAACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGCT2930.16437218800139128No Hit
GCCACAACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTA2910.16325019354404388No Hit
GCCACAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2820.15820121848598068No Hit
GCCACAACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGCTGAG2790.1565182267999596No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTCCTAAACCTCAACA2790.1565182267999596No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAG2790.1565182267999596No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACA2780.1559572295712859No Hit
GCCACAACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGCTGAGA2740.15371324065659117No Hit
GCCACAACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAGCTG2720.15259124619924377No Hit
GCCACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2610.14642027668383317No Hit
GCCACAACTGGGATTAGATACCCCACGAGACGTCTAGAGGAGCCTGTTCT2610.14642027668383317No Hit
GCCACAACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGCTGAG2460.13800531825372783No Hit
GCCACAACTGGGATTAGATACCCCTTTTACATGTAAGGTCCTAGAGGAGC2460.13800531825372783No Hit
GCCACAACTGGGATTAGATACCCCAAGAGTGCTGGCGGAATTCTTCTAAG2400.1346393348816857No Hit
GCCACAACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGC2390.134078337653012No Hit
GCCACAACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAGCTGAG2380.1335173404243383No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTAGAGGAGCCTGT2330.13071235428096986No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACA2330.13071235428096986No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG2320.13015135705229616No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC2270.12734637090892773No Hit
GCCACAACTGGGATTAGATACCCCTCTGTGCTTAGCCCTAAACCCTAACA2250.12622437645158033No Hit
GCCACAACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTAGCTGAG2190.12285839307953819No Hit
GCCACAACTGGGATTAGATACCCCCGACAACTAGAGGAGCCTGTTCTAGC2130.11949240970749604No Hit
GCCACAACTGGGATTAGATACCCCCCCTCCAAATCTCAGCCTAGGGTCCA2050.11500443187810652No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTCGATA1930.10827246513402224No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAACCCTAACA1870.1049064817619801No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAACA1830.10266249284728533No Hit
GCCACAACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTAGCT1820.10210149561861163No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGGG305.456968E-12294.988775
TGGGTTA206.171467E-8294.988779
AATGGGA550.0294.988776
CCACTGG108.460002E-4294.988775
CGGGATT255.293259E-10294.988778
CAATGGG550.0294.988775
CTGGGTT255.293259E-10294.988778
CTGGAAT108.460002E-4294.988778
CAACTGG175150.0294.904545
AACTGGG173750.0294.90396
ACTGGGA174400.0293.466467
CTGGGAT174250.0293.465158
GCCACAA178100.0293.166841
TGGGATT174350.0292.366279
CCACAAC182100.0285.51222
ACAACTG181300.0285.307684
CACAACT181750.0285.25053
CTGGATT1200.0282.697578
TGGGATA600.0270.406379
CACTGGG1000.0265.48995
Produced by FastQC (version 0.11.5)