FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-210-6-4-6-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-210-6-4-6-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173310
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA7066040.770873002134906No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA115286.651664647164042No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA63273.6506837458888697No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT57163.298136287577174No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA47882.7626795914834688No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG38972.2485719231435No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGACGTGGCT38742.2353009058911777No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA36022.078356701863713No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATA27531.5884830650279846No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA23391.349604754486181No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA22811.3161387109803242No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA22741.3120997057296175No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA19781.1413074836997288No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA19471.1234204604465985No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA14840.8562691131498471No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA14150.8164560613928797No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT13370.7714500028850038No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGATG13150.7587559863827823No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGACGTG11870.6848998903698574No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAG9540.5504587155963303No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA9510.5487277133460273No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA9490.5475737118458254No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA8930.5152616698401707No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTTATG8140.4696786105821938No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAACA7930.4575615948300733No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT7480.4315965610755294No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG7210.41601754082280307No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATA6960.4015925220702787No Hit
GCCACAACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATA6700.38659050256765337No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACA5950.3433154463100802No Hit
GCCACAACTGGGATTAGATACCCCACTACGCTTAGCCCTAAACTCCAATA5800.3346604350585656No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCATAAACATTGACA5800.3346604350585656No Hit
GCCACAACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAGA5580.3219664185563441No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC5540.3196584155559402No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA5310.3063873983036178No Hit
GCCACAACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTAGACG5080.2931163810512954No Hit
GCCACAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGACGTG5020.2896543765506895No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCGTAAACCTTGATA4950.2856153712999827No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG4680.2700363510472563No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGACGTGGC4410.25445733079453003No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGCAAACTTTGATA4160.24003231204200565No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGACA4150.23945531129190467No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATA4140.2388783105418037No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAGACGTGG3840.22156828803877446No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATA3710.21406727828746178No Hit
GCCACAACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGACGTGG3580.2065662685361491No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAGATA3570.20598926778604812No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTGCAACA3540.20425826553574522No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCAAAACCTCAACA3410.19675725578443254No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGACGT3360.19387225203392763No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAGATA3190.18406323928221105No Hit
GCCACAACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA3180.1834862385321101No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTTGGTA2950.17021522127978767No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGTA2920.16848421902948474No Hit
GCCACAACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAGAC2880.16617621602908084No Hit
GCCACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2800.16156021002827303No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTATAATA2780.16040620852807108No Hit
GCCACAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2620.15117419652645547No Hit
GCCACAACTGGGATTAGATACCCCGTCTCGTCTAGAGGAGCCTGTTCTAG2570.1482891927759506No Hit
GCCACAACTGGGATTAGATACCCCTGACTAGAGGAGCCTGTTCTAGACGT2560.14771219202584965No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAACA2550.14713519127574867No Hit
GCCACAACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT2500.1442501875252438No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA2370.1367491777739311No Hit
GCCACAACTGGGATTAGATACCCCTCGGAGATGTGTATAAGAGGAGCCTG2250.1298251687727194No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACATTGGCA2230.12867116727251746No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAGCCCTAGACCTCAACA2210.1275171657723155No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGACGT2130.1229011597715077No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATAGAAT1960.11309214701979112No Hit
GCCACAACTGGGATTAGATACCCCGTCTCTCTAGAGGAGCCTGTTCTAGA1950.11251514626969014No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA1830.10559113726847846No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTCCTAAACCTCAACA1820.10501413651837749No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTCGATA1810.1044371357682765No Hit
GCCACAACTGGGATTAGATACCCCTCTTCGGCAGCGTCAGATGTGTATAA1790.10328313426807455No Hit
GCCACAACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGA1760.10155213201777163No Hit
GCCACAACTGGGATTAGATACCCCCTCGTCGGCAGCGTCAGATGTGTAGA1750.10097513126767065No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAATA1740.10039813051756967No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGATT206.17074E-8294.992229
ACTTGGA206.17074E-8294.992227
TGGGTTA255.293259E-10294.992229
CACTGGG600.0294.992225
CTTGGAT206.17074E-8294.992228
TGGGGAT108.459507E-4294.992229
CAACTTT157.230541E-6294.992225
CTGGGTT305.456968E-12294.992228
GACTGGG108.459507E-4294.992226
ACTGGGT305.456968E-12294.992227
CAACTGG170450.0294.73265
AACTGGG169500.0294.731146
ACTGGGA169750.0293.775767
CTGGGAT169850.0293.602788
GCCACAA173200.0293.37421
TGGGATT169850.0292.73449
CCACAAC176700.0286.227572
CACAACT177600.0284.361883
ACAACTG179750.0280.304144
CAATGGG650.0272.30055
Produced by FastQC (version 0.11.5)