FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-21-4-3-8-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-21-4-3-8-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12119
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATTGACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCT201916.659790411750144No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA180414.8857166432874No Hit
TGATTGACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAACTTCGC9257.632642957339715No Hit
TGATTGACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAACTT4463.6801716313227164No Hit
TGATTGACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCT3002.4754517699480156No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA2752.2691641224523473No Hit
TGATTGACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAACT2682.2114035811535606No Hit
TGATTGACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAACTT2572.1206370162554666No Hit
TGATTGACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCT2472.0381219572571996No Hit
TGATTGACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTC2411.9886129218582391No Hit
TGATTGACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAACTTCG2221.8318343097615315No Hit
TGATTGACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTC2211.8235828038617046No Hit
TGATTGACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTAA2141.7658222625629179No Hit
TGATTGACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAACTTC1961.6172951563660367No Hit
TGATTGACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAACT1451.1964683554748743No Hit
TGATTGACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCT1331.0974502846769536No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGA1321.089198778777127No Hit
TGATTGACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAACT1301.0726957669774735No Hit
TGATTGACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCA1281.05619275517782No Hit
TGATTGACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTC1200.9901807079792062No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAA940.7756415545837115No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGA940.7756415545837115No Hit
TGATTGACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAACT910.7508870368842313No Hit
TGATTGACACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTAACT850.7013780014852711No Hit
TGATTGACACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTA840.6931264955854444No Hit
TGATTGACACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCT750.6188629424870039No Hit
TGATTGACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAACTT740.6106114365871771No Hit
TGATTGACACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTAACT740.6106114365871771No Hit
TGATTGACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTC710.585856918887697No Hit
TGATTGACACTGGGATTAGATACCCCTGTAGCTAGAGGAGCCTGTTCTAA670.5528508952883902No Hit
TGATTGACACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAAC550.45383282449046947No Hit
TGATTGACACTGGGATTAGATACCCCACGGGACCTAGAGGAGCCTGTTCT510.4208268008911626No Hit
TGATTGACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTT510.4208268008911626No Hit
TGATTGACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTA500.4125752949913359No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAA490.4043237890915092No Hit
TGATTGACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAA480.39607228319168253No Hit
TGATTGACACTGGGATTAGATACCCCTGATCTGCTAGAGGAGCCTGTTCT470.38782077729185577No Hit
TGATTGACACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGAC470.38782077729185577No Hit
TGATTGACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTA400.3300602359930687No Hit
TGATTGACACTGGGATTAGATACCCCCACCCTAGAGGAGCCTGTTCTAAC390.321808730093242No Hit
TGATTGACACTGGGATTAGATACCCCCGTACGTGCAGGGAGGGTACCTTG380.3135572241934153No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATC370.30530571829358855No Hit
TGATTGACACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAAC370.30530571829358855No Hit
TGATTGACACTGGGATTAGATACCCCTGCTGCAACTAGAGGAGCCTGTTC340.28055120059410843No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAA340.28055120059410843No Hit
TGATTGACACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTAACTT330.2722996946942817No Hit
TGATTGACACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGC320.264048188794455No Hit
TGATTGACACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTAAC310.25579668289462826No Hit
TGATTGACACTGGGATTAGATACCCCATGTGTATAGAGGAGCCTGTTCTA300.24754517699480155No Hit
TGATTGACACTGGGATTAGATACCCCAACGACCTAGAGGAGCCTGTTCTA300.24754517699480155No Hit
TGATTGACACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTAACTT280.2310421651951481No Hit
TGATTGACACTGGGATTAGATACCCCACGAGAGCTAGAGGAGCCTGTTCT280.2310421651951481No Hit
TGATTGACACTGGGATTAGATACCCCCCACCTAGAGGAGCCTGTTCTAAC270.22279065929532138No Hit
TGATTGACACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAAC260.21453915339549468No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAA260.21453915339549468No Hit
TGATTGACACTGGGATTAGATACCCCTAGTTGACTAGAGGAGCCTGTTCT250.20628764749566794No Hit
TGATTGACACTGGGATTAGATACCCCGAAACCTAGAGGAGCCTGTTCTAA240.19803614159584126No Hit
TGATTGACACTGGGATTAGATACCCCCGCCTAGAGGAGCCTGTTCTAACT230.1897846356960145No Hit
TGATTGACACTGGGATTAGATACCCCCAAACCTAGAGGAGCCTGTTCTAA230.1897846356960145No Hit
TGATTGACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAA230.1897846356960145No Hit
TGATTGACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAG230.1897846356960145No Hit
TGATTGACACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTAA230.1897846356960145No Hit
TGATTGACACTGGGATTAGATACCCCGATGTCCAGAGGAGCCTGTTCTAA210.1732816238963611No Hit
TGATTGACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTAACTT210.1732816238963611No Hit
TGATTGACACTGGGATTAGATACCCCCGACCAACCTAGAGGAGCCTGTTC200.16503011799653436No Hit
TGATTGACACTGGGATTAGATACCCCAAACCTAGAGGAGCCTGTTCTAAC190.15677861209670765No Hit
TGATTGACACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTAA190.15677861209670765No Hit
TGATTGACACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGAT180.14852710619688092No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAA180.14852710619688092No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGA180.14852710619688092No Hit
TGATTGACACTGGGATTAGATACCCCCGCCAAACCTAGAGGAGCCTGTTC180.14852710619688092No Hit
TGATTGACACTGGGATTAGATACCCCGAATAGTCCTAGAGGAGCCTGTTC170.14027560029705421No Hit
TGATTGACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAA170.14027560029705421No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCC160.1320240943972275No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAA150.12377258849740078No Hit
TGATTGACACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAACTT150.12377258849740078No Hit
TGATTGACACTGGGATTAGATACCCCCGAGAAGACCTAGAGGAGCCTGTT150.12377258849740078No Hit
TGATTGACACTGGGATTAGATACCCCGGTAGCTAGAGGAGCCTGTTCTAA150.12377258849740078No Hit
TGATTGACACTGGGATTAGATACCCCCCATCTAGAGGAGCCTGTTCTAAC140.11552108259757406No Hit
TGATTGACACTGGGATTAGATACCCCCGAGCTAGAGGAGCCTGTTCTAAC140.11552108259757406No Hit
TGATTGACACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTC140.11552108259757406No Hit
TGATTGACACTGGGATTAGATACCCCACCCTAGAGGAGCCTGTTCTAACT130.10726957669774734No Hit
TGATTGACACTGGGATTAGATACCCCCGGACCTAGAGGAGCCTGTTCTAA130.10726957669774734No Hit
TGATTGACACTGGGATTAGATACCCCCACACCTAGAGGAGCCTGTTCTAA130.10726957669774734No Hit
TGATTGACACTGGGATTAGATACCCCGACGAACCTAGAGGAGCCTGTTCT130.10726957669774734No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTGAC12000.0295.02
TTGACAC12000.0293.770844
TGATTGA12100.0292.561981
ACACTGG12000.0292.54177
CACTGGG11850.0292.510538
ACTGGGA11850.0292.510539
ATTGACA12150.0291.358033
GACACTG12150.0290.144046
TGACACT12300.0286.605685
CACTGGA202.2269402E-5221.249988
ACTGGAT202.2269402E-5221.249989
GCGGTGC2250.059.000004115-119
CTTAGCC1850.059.00000430-34
TGCTTCA1550.059.000004120-124
GCTCGCC1550.059.00000470-74
ACCCCAC6250.059.00000420-24
TACCCCA7100.059.00000420-24
CCCCACG3600.059.00000420-24
GCTTAGC1800.059.00000430-34
ACTGCTC1350.059.065-69
Produced by FastQC (version 0.11.5)